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The molecular phylogenetic trees were constructed by the NJ method using software MEGA4.0, and Portunus was clustered with Callinectes first, then they were clustered with Charybdis, and finally with Eriochier sinensis. The results are consistent with classical taxonomy.

用MEGA4.0软件中的NJ法构建了系统进化树，基于16S rRNA和COⅠ2种片段的聚类结果均显示梭子蟹属与美青蟹属亲缘关系最近，先聚在一起，然后再与蟳属聚在一起，最后才与外群中华绒螯蟹聚在一起，这一结果与传统分类基本一致。

When an enterprise wants to establish a representative office in Shanghai, it should apply to Shanghai Administration of Industry and Commerce on its website for prejudication first. It will approve or overrule the application in 5 working days.

一、外国企业申请在中华人民共和国境内设立常驻代表机构，须先向上海市工商局提出网上申请，工商局对其申请进行审查，在5个工作日内做出批准或不予批准的决定，并在网上公布结果。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02鞹A 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

Primary culture of goat mammary gland cells could be derived by outgrowth of migrating cells from fragment of tissue. Fibroblast and epithelial cells could be pured according to their different sensibility to trypsin. Complex cultures treated by 0.25% trypsin in Hanks' for 5~7min at 37℃, the dispersed cells were mainly fibroblasts,then the rest treated by 0.15% trypsin-0.02% EDTA in Hanks'for 5~8min at 37℃, the majority cells harvested were mammary epithelial cells.

用组织块培养法可获得良好的山羊乳腺细胞原代培养物；培养的山羊乳腺成纤维细胞比上皮细胞对胰蛋白酶更敏感，据此可在传代过程中将二者分离纯化，获得纯细胞系；混合培养物先用0.25%胰蛋白酶在37℃消化5~7min 所收集的细胞主要为成纤维细胞；再加0.15%胰蛋白酶-0.02%EDTA 消化液在37℃继续消化5~6min 所回收的细胞绝大多数为上皮细胞，经过2~3 代，即可得到纯化的乳腺上皮细胞系。

It's the first time that I saw Youyou sat in sofa and smiled to me when I opened the door, and she was the most talkative one than others at that night, sounds Shasha was more indrawn than her… Manything changed include Yixia's class rank from around 400th to around 20th, Shasha's unfluent reading English to fluent, and Youyou from indrawn to cheerful… I found that you should not trust what you saw or what you heard on the treatment of the children, the only thing you can do is just to trust future and change , and remember that nothing is impossible for children since they full of energetic.

第一次在进家门的时候看到优优坐在客厅的沙发上面带笑容地和我打招呼，然后一晚上她的话比谁都多，反而显得莎莎比较内向了似的…从以先由400多名上升到20多名，莎莎由英语阅读吃力到阅读流利，以及优优的内向到开朗，我总结出对孩子永远不要相信你看到的和听到的，要相信未来，相信改变，孩子是最能发展的生命力，在孩子身上没有什么不可能发生，如果你不是对孩子的缺点特别喜欢的话，那就尤其要学会忘记或者无视，因为那些真的很快就会过去

The agitation power consumptions in boiling systems decrease uniformly with the increase of agitation speed, whereas the relative power demand (RPD-the ratio of agitation power input of HEDT for gassed system to that for ungassed system and under the same agitation speed) can maintain at a high level for high gas loadings even when the impeller is operating with a high impeller tip speed. The gas hold-up in the boiling system is much lower than that in the cold gassed system, and the gas hold-up in boiling system increases at first and then decreases with the increase of the agitation power consumption per liquid mass P(subscript m. It implies that the boiling three-phase system has a maximum gas hold-up point. The changes of nucleation site of vapour bubble and the character of suspension of the solid particles with the rotational speed of the impeller are responsible for those behaviors mentioned.

在沸腾条件下，搅拌功率消耗随着搅拌转速的增加有所下降，但HEDT桨具有较高的载气能力，即使在高叶端线速度下，相对功率消耗仍能保持在较高的水平；沸腾体系的气含率随单位质量流体输入功率的增加表现出先增后减的规律，即存在气含率的极大值，蒸汽成核位置和固体颗粒悬浮特性随搅拌功率增加而改变是造成上述行为的主要原因；沸腾体系中的气含率远远低于常温通空气体系。

The carbons in explosives are decomposed into gas-like carbon atoms in the detonation reaction zone, then unoxidized carbon atoms coagulate into carbon liquid droplets and finally consolidate into solid diamonds The new mechanism differs from one conclusion that carbons of explosives are at first transformed into solid graphites, then into diamonds, and also differs from the conclusion that gaseous carbons coagulate into liquid droplets in the reaction zone, then crystalize into diamonds in Taylor rarefaction wave zone.

修正了有关炸药中的碳在反应区内先转变为石墨，然后石墨再发生相变生成金刚石的论点以及认为在反应区内气态碳凝聚为碳液滴，液滴在Taylor稀疏波区凝固成金刚石的论点。

First change in 840 (large Wayao Village - Tai Wayao Village) in the benefits the new East Street Station, in the east bridge stop; final transfer 976 (North Lake Drainage - earth station) in Shiliquan Home Station, in the pear orchard stop.

先换乘 840内在惠新东街站上车，在东风桥站下车；最后换乘 976支在十里居站上车，在梨园站下车。

After nursing for less than two weeks, and gaining up to a pound every five hours on its mother's fat-rich milk, a chubby seal pup called a whitecoat is ready to take his first swim in the North Atlantic.

Icy Stare 为生存而胖在加拿大的圣罗伦斯湾，一只小海豹在积冰的缝隙间试探海水。菱纹海豹几乎一出生就会游泳，但是却必须先花个两周时间储存脂肪，才可以在冰冷的海水中存活。

As to the defect divide,the thesis discussedfirst the problem of the most complex---design defect,and the focal point isdiscussing the new development about design defect in the Restatementof Torts,which means that in the standard of judgment we should adoptnegligence instead of strict liability,and that in adducing evidence TheReasonable Alternative Design should be put forward.

在缺陷的划分上，论文先论述了最为复杂的设计缺陷的问题，重点论述了美国《第三次侵权法重述》关于产品设计缺陷的新发展，即在判断标准上采疏忽责任而非严格责任，在举证上要求提出合理的可替代的设计问题。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新