先

B:Oocytes and early embryos were permeated in 0.5%Triton X-100 for 10 minutes,then fixed in 4% paraformaldehyde/PBS.Method C:Oocytes and early embryos were fixed in 1%PFA while permeated in 0.2%Triton X-100 at the same time for 1 hour.Method D: Oocytes and early embryos were fixed in precooled methanol(-20℃) for 15 min,then permeated in 0.5%Triton X-100 for 10 minutes.

方案A：先用4%多聚甲醛固定1h，再用0.5%Triton X-100穿透10min；方案B：先用0.5%Triton X-100穿透10min，再用4%PFA固定1h；方案C：用1%PFA+0.2%Triton X-100同时固定与穿透1h；方案D：用-20℃预冷甲醇固定15min，再用0.5%Triton X-100孵育10min。

Change when accumulator, must notice to be distinguished first losing terminal, tear open the jumper on negative pole column first next, tear open again

更换蓄电池时，一定要注重先分清正负极柱，然后先拆负极柱上的连接线，再拆

In the first simple and easy after the first host of the principles of the power jack plug for a power test, the null and void, it will display normal use to remove the power cord to connect the host, the host returned to normal.

本着先简单后容易的原则先将主机的电源插头换一个电源插孔试之，无效，便将正常使用的显示器电源线取下连接主机，主机恢复正常。

"Encumbrance" means any claim, charge, mortgage, lien, option, equity, power of sale, hypothecation, usufruct, retention of title, right of pre-emption, right of first refusal or other third party rights or security interest of any kind or an agreement, arrangement or obligation to create any of the foregoing and "Encumber" shall be construed accordingly

&阻碍&指任何索赔，收费，抵押，留置，选择，资产净值，销售权，担保契约，使用权，权利保持力，先占权，先拒权，或者其他第三方权利或者任何种类的担保物权，或者为了创造前述的任何方面的一个协议，安排或者义务，阻碍要针对性地解释。

In ancient Japan, when a new student seeks admission to study at a monastery, he is required to have an audience with the master, a sort of pre-entrance interview if you will.

在古代的日本，在古代的日本，新人要想进入寺院习修禅必须先与方丈大师举行会谈，法，必须先与方丈大师举行会谈，类似于入学前的面试。

Recently, a complex congenital heart disease in young infants in Beijing Children's Hospital to accept the "one and a half ventricular repair" operation (the child's right ventricular dysplasia, is not able to meet the functions of his heart, and he was part of the right ventricle to the blood to the road directly to the pulmonary artery, the other part to go through the right ventricle), spent a total of more than 60,000 yuan,"PapaS" fast food companies responsible for 4 million yuan before the children have recovered and been discharged.

最近一个复杂先心病小婴儿在北京儿童医院接受了&一个半心室修复&的手术（这个孩子的右心室发育不良，不能够满足他的心脏功能，把他本来到右心室的一部分血改路，直接到肺动脉，另外一部分还要经过右心室），共花费了6万多元，&PapaS&快餐公司担负了4万多元，先该患儿已康复出院。

Print ribbon is installed, we need to print frame first uninstall it from the printer, uninstalling the device must be near the print head from the location you pull up hard, in order to easily print aircraft had come from the printer ; the following, we also need to open the print frame, taking into account different models of printers that it installed on the structure of the printing frame is not exactly the same, so the installation of ribbon winding method is not exactly the same, we have to uninstall it before the original band, we a good look around the ribbon before the correct method, or else do not print the right way to install the ribbon will not be able to function properly, but also may lead to the printer not work correctly.

在安装打印色带之前，我们需要先将打印架从打印机中卸载下来，在卸载该设备时，必须先从靠近打印头的位置处用力向上拉一下，才能把打印架轻松地从打印机中拿下来；下面，我们还需要将打印架打开，考虑到型号不同的打印机它的打印架安装结构并不完全相同，因此色带的安装缠绕方法也不完全相同，我们在将原色带卸载下来之前，一定要好好地观察一下色带以前的正确绕法，否则不按正确方法安装的打印色带不但不能正常发挥作用，相反还有可能导致打印机无法正常工作。

Should be in power after the first pull circuit breakers pull isolation switch to send electricity to be first combined together after the circuit breaker switch.

在停电时应先拉断路器后拉隔离开关送电时应先合隔离开关后合断路器。

When they opened her up, they found two tiny wrinkle-faced boys bowing to the waist and saying to each other politely,"After you, brother, after you."

当他们把她剖开时，发现有两个小小的、脸皮皱皱的男孩，在哈腰鞠躬，还互相有礼貌地说：「请先，哥呀，请先。

Came to the lava cliffs fall zone, take the left side of the road directly in a group of birds where there are two columns below, the jump down (not dead, I am sleepy in this place as long as two hours), skip left after the first go, be reminded to observe, and now moving about in the magma, but can only be waist high magma, there is a need to see more of the terrain, do not waste HP, now jumped down, back to the left跳崖Department, went to is located in Magma, Fire Dragon Shou equipment can move around a half height of magma, first up, get a weapon, and then down, has been in the end, to continue filing filed walk inside the wind can be magic, the way to the red chest is a green precious stones, to increase the wind magic attack, and get back only to return to bandage things MM has emerged, but said a few left, and then returning to fight BOSS, the BOSS to attack his tail, the beginning of the head is of no use, finished BOSS, while red chest after using wind magic jump can be achieved

掉下悬崖来到了熔岩地带，直接走左边的路，在一群鸟的地方，下面有两根柱子处，往下跳(不会死的，我在这个地方困了2个小时之久)，跳下后先往左走，得到火龙守，现在可以在岩浆中走动了，但是只能是下半身高的岩浆，所以要多看地形，不要白白浪费HP，现在可以往下跳了，回到跳崖处往左，来到岩浆地处，装备火龙守，可在半身高岩浆里走动，先往上，拿到武器一把，再往下，一直到底，存档处存档继续往里走可得到风魔法，途中的红宝箱是绿宝石，增加风魔法攻击的，等回来时才能拿，返回事绷带MM又出现了，不过说了几句就走了，然后返回途中打BOSS，此BOSS要攻击他的尾巴，开始时打头是没有用的，打完BOSS，途中红宝箱利用跳后的风魔法可以取得

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。