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Calvaria was obtained and scissored into pieces on the super clean blench through sterile method after fetal Kunming mouse within 24 hours were killed by cervical dislocation in the experiment.

试验采用24h内昆明乳鼠,拉颈处死,无菌取出头盖骨,在超净工作台内将其剪碎,用0.25%胰蛋白酶37℃消化30min,然后用0.2%胶原酶37℃振荡消化60min,反复吹打骨片分离更多细胞。

Calvaria was obtained and scissored into pieces on the super clean blench through sterile method after fetal Kunming mouse within 24 hours were killed by cervical dislocation in the experiment. The bone pieces were digested by 0.25% trypsin for 30 min at 37℃ at first, then digested by 0.2% collagenase at 37℃ in constant temperature oscillator for 60 min, and rinsed repeatedly to disaggregate more single-cells.

试验采用24h内昆明乳鼠,拉颈处死,无菌取出头盖骨,在超净工作台内将其剪碎,用0.25%胰蛋白酶37℃消化30min,然后用0.2%胶原酶37℃振荡消化60min,反复吹打骨片分离更多细胞。

Methods Randomized block design was used in this study. A total of 74 subjects were enrolled.

共入选74例患者,采用随机分组的方法,分层,然后随机分为两组。

They began by building blocks of flats for workers.

他们给工人盖一片片的住宅。

November 1120 along with his wife, Mathilde of Blois, by whom he had no

在订阅设置页上更新您的昵称,然后再进行发帖。

If so, can I bank my blood before surgery?

如果需要,我可否在手术前存留自己的血液?

Take your blood count first, please.

做一次球计数。

I think if you've got a T-shirt with a bloodstain all over it, maybe laundry isn't your biggest problem.Maybe you should get rid of the body before you do the wash.

我在想,如果你的衬衫上面染了血迹,这时候也许洗衣服已经不是你迫在眉睫的问题了,也许在去洗衣服前你应该考虑如何摆脱那具尸体。

After no-hitting the Blue Jays through three innings, David Eckstein touched him for a bloop double to lead off the fourth.

在蓝鸟三局无安打之后,David Eckstein在四上击出一支二垒安打。

M. ' | The Yankees won a game last week playing National League-style small ball, bunting a runner to third base and setting the table for a bloop hit that pushed the go-ahead run across.

S3 ' V5 Y; w 洋基队上星期靠著国联式的小球打法赢了一场球,以短打送跑者上三垒形成得分契机,再打出一球落在内外野之间,强攻下超前分。

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Do you know, i need you to come back

你知道吗,我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。