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Abstract] Objective To study a RP-HPLC methods that determined the protocatechuic acid content in Fuxuekang Jiaonang.

摘要] 目的建立反相高效液相色谱法测定妇血康胶囊中原儿茶酸含量。

Methodsa gradient hplc system was used in the quantitative analysis of the danshensu ,protocatechuic aldehyde and salvianolic acid b in 6 hours,and its ph was determined at 25℃for 24 hours.

方法采用高效液相色谱法梯度洗脱测定配伍液中6 h内丹参素、原儿茶醛、丹酚酸b的含量。并在25℃时24 h测定配伍液的ph值。

Holly leaf containing protocatechuic acid, there is Yang bacteria.

冬青叶含有原儿茶酸,有仰菌作用。

Objective To study the effects of carbon, nitrogen, and phosphate sources on the growth of Salvia miltiorrhiza adventitious roots and the contents of tanshinone Ⅱ and protocatechuic aldehyde.

目的 研究碳源、氮源和磷源对丹参Salvia miltiorrhiza不定根生长及其有效成分丹参酮Ⅱ和原儿茶醛量的影响。

Results Protocatechuic acid has linear correlation between 0.103 μg and 0.7725 μg,the results also have shown the recovery rate was 97.76%,RSD 1.01%.

结果 原儿茶酸在0.103~0.7725 μg范围内呈良好的线性关系;平均回收率为97.76%,RSD=1.01%。

Methods:The RP-HPLC method was applied and protocatechuic aldehyde was used as the internal standard substance.

采用RP-HPLC法,以原儿茶醛为内参照物,色谱柱:Luna C_(18)柱(250mm×4.6mm,5μm),流动相:0.05%磷酸水溶液-乙腈,梯度洗脱程序为:0min时A-B(100∶0),80min时A-B(67∶33),85min时A-B(60∶40),100min时A-B(60∶40),流速:1.0mL·min~(-1),检测波长:280nm。

Results Protocatechuic acid has better linear correlation between 0.103 μg and 0.7725 μg,the results also have shown the recovery rate was 97.76%,RSD 1.01%.

结果 原儿茶酸在0.103~0.7725 μg范围内呈良好的线性关系;平均回收率为97.76%,RSD=1.01%。

A new method was reported for the determination of tanshinol and protocatechuic aldehyde in dripping solution by microfluidic chip with contactless conductivity detection.

建立了微流控芯片非接触电导检测法测定丹参滴注液中丹参素与原儿茶醛含量的分析方法。

Results Protocatechuic acid has better linear correlation between 0.103 μg and 0.7725 μg,the results also have shown the recovery rate was 97.76%,RSD 1.01%.

效果 原儿茶酸在0.103~0.7725 μg领域内呈良好的线关连;均匀接纳率为97.76%,RSD=1.01%。

Results There were almost no changes in pH and the contents of danshensu、protocatechuic aldehyde in compatible solution within 6 hours.

结果注射用丹参与0.9%氯化钠注射液、5%葡萄糖注射液配伍后溶液6h内pH值变化较小;配伍液不溶性微粒数远远高于原注射液的不溶性微粒数(P.01);主要成分丹参素钠和原儿茶醛的含量基本无变化。

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