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Several inducers cicada exuviae, colloidal chitin, horsefly cuticle, shrimp, maggot and silkworm chrysalis were compared with their effects on production of cuticle-degrading protease of entomopathogenic fungus Metarhizium anisopliae.

研究了不同碳氮源对金龟子绿僵菌产生与分解昆虫外壳相关的蛋白酶活性和同工酶谱带的影响。结果表明:蝉蜕、虻虫壳、蝇蛆壳、蚕蛹壳、虾壳、胶体几丁质均可使金龟子绿僵菌产生蛋白酶,虾壳和胶体几丁质所产生的蛋白酶比活性最高,蝉蜕次之。

Different biological and ecological characteristics, virulence, sporulation, conidia bourgeon, Protea-Bse activity leve and colony growth of 6 strains of Beauveria bassiana were measured respectively. The results were calculated by grey relational analysis and the optimum sequence was built from the professional comparison of each strain's characteristics. Two strain's have a potential of appling in mass production, BD-B015 and BD-B038 were abtained. The revisory mortality to Laphygma exigua Hubner were 88.33% and 86.70%.The infected rate were 86.67% and 85.27%.

测定初筛获得的6个球孢白僵菌菌株的毒力、产孢量、萌发率、胞外蛋白酶活性及菌株生长速度等多个生物、生态学指标,从专业角度构建最优序列,经灰色分析和不同菌株毒力效价显著性比较,筛选出了两株具有较大生产潜力的优良菌株BD-B015和BD-B038,对甜菜夜蛾的校正死亡率分别为88.33%和86.70%,僵虫率分别为86.67%和85.27%。

The effect of 6 fungicides on germination of conidia of the strain Ma83, Metarhizium anisopliae, in different time was studied.

以孢子的萌发率为指标,在涂有一层杀菌剂药膜的琼脂表面接种金龟子绿僵菌Metarkizium anisopliae的Ma83菌株孢子悬浮液,研究6种常用化学杀菌剂使用后不同时间对绿僵菌Ma83萌发的影响。

The results showed that the mortality of the larvae caused by infection of Metarhizium anisopliae increased with the concentration of conidial suspension and the time after treatment.

结果表明,分离出的虫生真菌为金龟子绿僵菌菌株;随金龟子绿僵菌孢子浓度的升高,华北大黑鳃金龟幼虫的感病死亡率增加,在浓度为2.1×104、1.9×105、2.2×106、2.0×107、1.8×108个孢子/ml时,幼虫的累计死亡率分别为20 %、65 %、90 %、100 %和100 %。

Content of (NH_4)_2C_2O_4 in the white muscardine silkworm is mensurated primely by oscilloscopic potentiometric titration, being independent of colour and turbidity.

白僵蛹与白僵蚕草酸铵含量的相似决定了它们疗效的相似,因此自僵蛹可以代替白僵蚕使用。

We use 0.5% CMC to preserve the activity of Metarhizium anisopliae at room temperature. Then we carry on the RT-PCR semi-quantitative examination of the trehalase. The fresh Metarhizium anisopliae not preserved do control.

对用0.5%的羧甲基纤维素钠室温保存五个周的绿僵菌,进行海藻糖的半定量RT-PCR检测,用没有经过处理的固体培养基上培养14天的绿僵菌做对照。

We use 0.5% CMC to preserve the activity of Metarhizium anisopliae at room temperature. Then we carry on the RT-PCR semi-quantitative examination of the trehalase. The fresh Metarhizium anisopliae not preserved do control.

对用0.5%的羧甲基纤维素钠室温保存五个周的绿僵菌,进行海藻糖酶的半定量RT-PCR检测,用没有经过处理的固体培养基上培养14天的绿僵菌做对照。

The results showed that the conidia of the RNAi transformant accumulated more trehalose, lost viability more slowly during prolonged storage and showed reduced heat shock sensitivity than the wild type, and the overexpression transformant has reverse results. These results indicate that neutral trehalase in M.anisopliae control trehalose concentration via degradation of trehalose, and indirectly influnce the viability of conidia during prolonged storage and the resistance to heat stress.

以上研究结果表明,金龟子绿僵菌CQMa102孢子体内海藻糖是由中性海藻糖酶降解的,海藻糖含量与耐储性和耐热性具有正相关性,中性海藻糖酶在虫生真菌金龟子绿僵菌孢子中的生理功能是通过降解分子内海藻糖调控海藻糖含量,从而影响其对不良环境的抗性,如储藏性、耐热性。

In an effort to immobilize the world, it immobilizes itself, undermining its own ability to cope with anything new or to resist the natural currents of life.

在使世界僵止不动的努力中,它也令自己僵止不动,暗中瓦解了自己对任何新生事物妥善处理或阻挡自然的生活之流的能力。

Data mining with decision tree analysis revealed that different combinations of morning stiffness, slight red tongue, joint tenderness and nocturnal polyuria in the CM group, and those of white furin tongue, CRP level, leucocyte count and morning stiffness in the WM group showed different efficacy, whichwere also verified in the randomly chosen verifying set.

决策树分析结果显示:中药治疗组中晨僵、舌淡红、关节压痛程度、夜尿多4项观测指标不同组合患者的中药治疗疗效有差异;西药组中舌苔白、C反应蛋白、白细胞数量和晨僵4项观测指标不同组合患者的中药治疗疗效有差异;同时,决策树分类的结果在随机选取的验证集中也得到了验证。

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