偶氮四唑

Myocardialinfarct size and the area at risk for myocardial infarctionwere measured by Evans Blue and triphenyl tetrazolium chloridestaining, respectively.

心肌梗死面积和可能发生心肌梗死的危险区分别可以用偶氮蓝与氯化三苯四唑染色测量。

Annonaceous acetogenins ; human liver cancer cell line HepG2; MTT

番荔枝内酯； HepG2肝癌细胞株；四甲基偶氮唑蓝

Methods:The proliferation inhibition of cantharides injections on LoVo cells were detected by tetrazolium.Annexin V-FITC were applied to study cell apoptosis of LoVo cells.FCM were applied to detect the expressions of the Fas and Bcl-2 protein.Results:Cantharides iniections could inhibit the proliferation of LoVo cells and could couse cell apoptosis.

四甲基偶氮唑盐测定药物对LoVo细胞抑制作用与时间和剂量的关系，Annexin V-FITC方法对细胞凋亡进行检测，流式细胞术检测细胞Fas、Bcl-2蛋白表达水平结果：MTT实验表明复方斑蝥注射液可抑制LoVo细胞的生长并诱导发生凋亡。

Methods The article applied the orthogonal design to find out the optimum proportion of the rutin, soybean isoflavone and curcumin. The free radical DPPH scaving ratio was used as evaluating standard of orthogonol design and the hominal liver cancer cell SMMU-7721 was used in MTT trial to evaluate the inhibitory ability of the compound. Results The optimum proportion of rutin-soybean isoflavone-curcumin 2:3:7 was achieved.

选取3种天然植物抗氧化物质芦丁、大豆异黄酮、姜黄素，利用正交试验设计选择合适配方，以自由基DPPH的清除率作为正交试验评价标准，并利用人肝肿瘤细胞SMMU-7721进行四甲基偶氮唑盐试验来考察配方体外试验对肿瘤细胞的抑制作用。

Methods The cytotoxic effects of the four kind extracts of Livistona chinesis on two tumor cell lines cultured in vitro were tested by MTT assay.

采用四甲基偶氮唑盐比色法，观察蒲葵子提取物的各萃取部位对肝癌细胞HepG2和白血病细胞HL60增殖的抑制作用。

Methods MTT Chromatography is adopted to test effects of 5-FU of different concentration and at different time of reaction over growth of Lovo cells; photomechanical method used to observe configuration and characteristics of apoptosis cells; FCM employed to test the percentage of apoptosis cells; electrophoresis and immunized grouping SP applied to test antigen Ki-67 of Lovo cells proliferation and expression of P53 and Fas genetic proteins in relation to apoptosis.

采用四甲基偶氮唑盐显色法，检测不同浓度5－FU作用不同时间对Lovo细胞生长所产生的不同影响；光镜观察凋亡细胞的形态特点；流式细胞术检测凋亡细胞百分率；琼脂凝胶电泳、免疫组化SP法检测 Lovo细胞增殖核抗原Ki－67及凋亡相关基因蛋白p53、Fas表达水平。

Methods The cultured 3T3 -L1 cells were pretreated with naringin, and then MTT method was used to detect the proliferation of the cells, oil red O staining method and spectrophotography were applied to analyze the degree of differentiation.

培养3T3-L1细胞，并用不同浓度的柚皮苷进行干预，以四甲基偶氮唑盐法检测细胞增殖，用油红O染色和染色比色法分析脂肪细胞的分化程度。

The effects of FLLPS on the immunoactivity of mouse speen lymphocyte were studied in vitro or vivo by means of MTT.

采用四甲基偶氮唑盐比色分析法，对其多糖成分进行了小鼠体内外免疫活性观察。

Trypan blue dye exclusion method and MTT assay were used to determine the uncytoxic dose ranges of PEA.

采用台盼蓝染色排除法及四甲基偶氮唑蓝法确定PFA对淋巴细胞的非毒性剂量范围。

Methods MTT was used to study the proliferation of A375 human melanoma cell. Tyrosinase activity was measured by zymologic method. The melanin was detected by its absorption at 475nm.

方法四甲基偶氮唑蓝法测定验方对细胞增殖的影响，用酶学方法测定对酪氨酸酶活性的影响，475nm比色法测定验方对黑素含量的影响。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。