偶氮四唑

After cultured with tumor cytolysis antigen for 2 days, the cells were incubated with auto T cell separated by E-rosette formation. Methyl thiazolyl tetrazolium assay was employed to test inhibition rate of tumor specific cytotoxic T lymphocyte on HO9810 tumor cell line. Results Our results indicated that DC precursors in cord blood can be induced to differentiate and mature in the medium containing IL-4, GM-CSF, and TNF-α.

将获得的DC与用 E-花环分离脐血单个核细胞得到的T淋巴细胞共同培养3 d，然后分为两组，实验组2继续与不同比例的HO9810细胞共同培养3 d后获得细胞毒T淋巴细胞，对照组2除加入HO9810外，还加入了无关细胞，用四甲基偶氮唑蓝法检测其对HO9810细胞的抑制作用。

After 4 passages in culture, the MSCs were implanted into 96 foveola with cell density of 0.08,0.1,0.2,0.4,0.6,0.8,1,2,4,6,8,10(x104/ml) respectively. Meanwhile, the MSCs were cultured in Dulbecco's modified Eagle's medium culture medium with different concentration of fetal bovine serum, the 3-4,5dimethylthiazol-2-yl-2,5-diphenyl tetrazelium bromide assay were used to determine the proliferation index of MSCs 72 hours later, the cell cycle were determine by cytometer also.

贴壁筛选法分离、纯化MSCs，取4代以后细胞，以不同密度接种于96孔培养板内，分别以不同浓度血清进行培养，72h后，采用四甲基偶氮唑盐比色法测定MSCs增殖能力并用流式细胞仪测定细胞周期。

Methods:Phenobarbital and Valproic acid were added into astrocytes and cultured permanently for 30 days. P-glycoprotein, a product of MDR1, was detected with immunocyto chemistry. Then, 5μg/ml Sibelium was added to resistance cells and nonresistance cells, MTT assay was used to measure reverse multiple and relative reverse efficiency rate.

将苯巴比妥钠和丙戊酸钠分别加入培养的星形胶质细胞内，30天后用免疫细胞化学法检测MDR1的表达产物P-糖蛋白，继之将终浓度为5μg/ml的西比灵分别加入培养的正常细胞及MDR1表达增强的细胞内，用四甲基偶氮唑盐法计算逆转倍数和相对逆转效率，了解西比灵逆转MDR1表达的作用。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

The antitumor effects of sesquiterpene lactones scabertopin and isodexyelephantopin from the whole plant of Elephantopus scaber were studied in vitro on SMMC-7721,Hela and Caco-2 cancer cell lines.

采用四甲基偶氮唑盐比色法和琼脂糖凝胶电泳法等研究地胆草倍半萜内酯化合物scabertopin(1)和isodeoxyelephantopin(2)在体外对SMMC-7721、Hela和Caco-2三种肿瘤细胞增殖的影响。

The inhibition on the growth of CAOV3 was tested by flow cytometry and methyl thiazolyl tetrazolium assay, etc.

通过四甲基偶氮唑蓝比色法及流式细胞术等，观察转染 Rb基因的CAOV3细胞的生长情况。

Using methyl thiazolyl tetrazolium to assay the cell viability and metabolism state.

用四甲基偶氮唑盐法测定细胞活力和代谢状态。

Proliferation of cells was analyzed by mitochondrial reduction of tetrazolium salt MTT to formazan and actual cell count.

用四甲基偶氮唑盐法和细胞计数法分析细胞增殖情况。

Methods hela cells were cultured in vitro and effects of sbti on cell proliferation were measured by using methyl thiazolyl tetrazolium assay.

选用hela细胞进行体外培养，采用四甲基偶氮唑蓝比色法检测sbti对细胞增殖的影响。

Methods: ECV-304 cells were injured by hydrogen peroxide and assessed by methyl thiazolyl tetrazolium assay.

用过氧化氢致ECV-304细胞损伤，以四甲基偶氮唑蓝比色法检测细胞存活数量。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。