偶氮四唑

A novel synthetic approach of 3,3'-azobis(6-amino-1,2,4,5-tetrazine) was firstly devised. Taking 3,6-bis(3,5-dimethylpyrazol-1-yl)-1,2,4,5-tetrazine as a primary material, DAAT was synthesized by high pressure ammonolysis and potassium permanganate oxidation. Compared with the four-step reaction in the literature, this new approach was reduced to two steps with a yield of 58.1%. The intermediate and DAAT were characterized by elemental analysis, IR and NMR spectra.

自行设计了3，3'-偶氮双(6-氨基-1，2，4，5-四嗪)新合成路线、采用3，5-双(3，5-二甲基吡唑-1-基)-1，2，4，5-四嗪为原料，由文献报道的4步反应缩减为2步，经高压氨解、高锰酸钾氧化合成了DAAT，总收率大幅提高，达到58.1%，并采用元素分析、红外光谱、核磁共振光谱等进行了结构表征。

Methods the rabbit arterial smooth muscle cells were cultured ex vivo, cells of 4-7 generation were divided into groups interfered with ang-（1-7）, positive control groupsinterfered with ang-ii(10-6mol/l) and blank control group .cell counting with trypanblau dying and mtt were used to evaluate the growth of cells in different groups and results were compared .

体外培养兔肺动脉平滑肌细胞，鉴定后传代培养，取4～7代细胞，应用不同浓度ang-（1-7）干预，并与血管紧张素-ii(ang-ii，浓度10-6mol/l)、空白组对照。通过台盼蓝染色法细胞计数、四甲基偶氮唑蓝比色法，确定肺动脉平滑肌细胞的生长趋势变化。

One hundred and eighty 14-days-old chickens were divided randomly into coccidia primary infection group (I1),coccidia secondary infection group (I2),and control group.Immune indexes were examined by using immune staphytococcal protein A rosette test,indirect enzyme-linked immunosorbent assay,cellular culture technique and MTT method.

将180只14日龄雏鸡随机分为毒害艾美球虫初次感染组、二次感染组和对照组，应用免疫SPA菌体花环、间接ELISA及细胞培养技术和四甲基偶氮唑盐测定法对相关免疫学指标进行了检测，以研究毒害艾美球虫二次感染对雏鸡外周血液免疫功能变化的影响。

objective to investigate the influence of dit on the proliferation of human thyrocytes and compare dit with ki.to study a good kind of supplement to usi that was safe and effective.methods the human thyroid epithelium cells from para-adenoma normal tissues from thyroid adenoma patients were cultured in the absence or presence of 10-8～10-3mol/l dit or ki.mtt method was employed to obtain the proliferation of cultured human thyrocytes.

取甲状腺腺瘤旁正常甲状腺组织，应用原代细胞培养技术进行细胞培养，以不同浓度(10-8～10-3mol/l)的dit和ki刺激单层培养的甲状腺细胞，采用四甲基偶氮噻唑蓝法测定各组甲状腺细胞的增殖情况。

The cell phenotype was observed at 6,12,24 and 48h and the cellular proliferation was examined by methyl thiazolyl tetrazolium method.

将幽门螺杆菌野生株与cagA基因缺失突变株与BGC823细胞共培养，分别在6，12，24和48h观察细胞形态学变化，采用四甲基偶氮噻唑蓝比色法检测细胞增殖活性，流式细胞仪检测细胞凋亡情况。

objective to isolate and purify the antimicrobial peptides with anti-toxoplasma gondii activity isolated from the haemlymph of musca domestica larvae.methods the antimicrobial peptides of musca domestica larvae were induced by infection and injury were isolated and purified by trituration,centrifugalization and column chromatography.then the antimicrobial peptides with anti-toxoplasma activity were sieved by mtt colorimetric method and haemacytometry.results it was found that two antimicrobial peptides had anti-toxoplasma activity through resource s cationic column chromatography and superdex g75 gel column chromatography.conclusion the two antimicrobial peptides with anti-toxoplasma activity existing in the haemlymph of musca domestica larvae had different anti-toxoplasma effect.

作者单位：山西医科大学寄生虫教研室，太原 030001；化学生物学与分子工程教育部重点实验室山西大学生物技术研究所目的从家蝇幼虫血淋巴中分离纯化具有抗弓形虫作用的抗菌肽。方法通过损伤加感染的方法诱导家蝇幼虫大量表达抗菌肽，然后经过研磨、离心和层析等过程，将家蝇幼虫抗菌肽进行分离纯化，采用四甲基偶氮噻唑蓝比色法和血细胞计数法筛选对弓形虫速殖子有抑制作用的抗菌肽。结果经resource s阳离子柱和superdex g75凝胶柱层析后，筛选出2种对弓形虫速殖子有杀伤作用的抗菌肽。结论家蝇幼虫血淋巴中存在抗弓形虫作用的抗菌肽，而且不止一种，但其效果有所不同。

objective effects of plant tannins,chebulinic acid and tellimagrandin i on chemically induced hemoglobin synthesis in k562 cells were investigated.methods the hemoglobin synthesis situation was assayed with benzidine staining.erythroid antigens glycophorin a expression on the surface of k562 cells was labeled by direct immunofluorescence using fluorescein isothiocyanate-conjugated anti-gpa antibodies.then flow cytometric analysis was performed to detect gpa expression levels on surface of the cells.results both chebulinic acid and tellimagrandin i could inhibit the hemoglobin synthesis of butyric acid and hemin-treated k562 cells in a concentration-dependant manner.however,the ba-induced k562 cells were more sensitive to two tannins than heroin-induced cells.conclusion chebulinic acid and tellimagrandin i have inhibitory effect on the erythroid differentiation.

目的 研究可水解单宁诃子酸和特里马素i对氯化高铁血红素和丁酸钠诱导k562细胞红系分化的影响。方法四甲基偶氮噻唑蓝法分析诃子酸和特里马素i对k562细胞生长的影响，联苯胺染色法检测血红蛋白合成情况，应用免疫荧光和流式细胞术检测血型糖蛋白a在细胞表面的表达。结果诃子酸和特里马素i在0.04～0.09？mmol／l浓度下均可显著抑制k562细胞的生长增殖。2种单宁化合物(0.002～0.01？mmol／l)可显著抑制丁酸钠诱导的k562细胞血红蛋白合成，2种单宁化合物(0.01～0.05？mmol／l)还可显著抑制氯化高铁血红素诱导的血红蛋白合成，特里马素i(0.01？mmol／l)还抑制丁酸钠诱导的gpa在细胞表面表达。结论诃子酸和特里马素i对红系分化有抑制作用。

Methods BGC823 cells were co-cultured with the wild type H.pylori and the isogenous mutant, which were called the wild tue strain group and the mutant stain group,respectively. The cell phenotype was observed at 6,12,24 and 48h and the cellular proliferation was examined by methyl thiazolyl tetrazolium method.The induction of apoptosis was determined by flow cytometry.

将幽门螺杆菌野生株与cagA基因缺失突变株与BGC823细胞共培养，分别在6，12，24和48h观察细胞形态学变化，采用四甲基偶氮噻唑蓝比色法检测细胞增殖活性，流式细胞仪检测细胞凋亡情况。

Methods BGC823 cells were co-cultured with the wild type H.pylori and the isogenous mutant,which were called the wild tue strain group and the mutant stain group,respectively.The cell phenotype was observed at 6,12,24 and 48h and the cellular proliferation was examined by methyl thiazolyl tetrazolium method.The induction of apoptosis was determined by flow cytometry.

将幽门螺杆菌野生株和cagA基因缺失突变株和BGC823细胞共培养，分别在6，12，24和48h观察细胞形态学变化，采用四甲基偶氮噻唑蓝比色法检测细胞增殖活性，流式细胞仪检测细胞凋亡情况。

Results At different time and concentration,genistein had different effects on osteoblastic cell proliferation.

用四甲基偶氮噻唑蓝比色法检测细胞增殖，碱性磷酸酶活性测定检测细胞的分化。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。