偏爱

The coden selection of the designed Cecropin AD gene was carried out according to the coden usage of yeast. The designed gene is 140 bp in length.

为适应酵母表达需要，基因中编码氨基酸残基的密码子以酵母偏爱密码为基础，借助DNASIS软件进行适当调整以消除不合适的密码子串联组织、潜在的二级结构和不适当的限制性内切酶位点。

According to the amino acid sequence of monellin and the bias in coden choice in Escheria coli,a single chain monellin gene was synthesized and subcloned into a Escheria coli expression vector pET-28a.

根据已报道的单链Monellin甜蛋白的氨基酸序列，按大肠杆菌基因偏爱密码子，设计和人工合成了单链monellin基因。

In this study, the DNA fragments coding for amino acids 133～158 of VP1and 20～34 of VP4protein of type Asial FMDV were chemically synthesized and ligated into a tandem repeat 133～158-20～34-133～158. The sequences of signal peptide of Igκ chain and Kozak were fused to the 5'end of this tandem sequence and synthetic oligodeoxynucleotide containing CpG-ISS was fused to downstream of terminal coden of this tandem sequence. And then this long fragment was cloned into the eukaryotic expression plasmid pHook-2, forming a new secreted expression plasmid, named pAS1-E.

在第二章证明了亚洲Ⅰ型口蹄疫病毒VP1蛋白中133～158位氨基酸残基确是一重要B细胞中和抗原表位的基础上，依据第一章获得的口蹄疫病毒亚洲Ⅰ型VP1 cDNA序列及已报道Asial VP4序列，采用真核偏爱密码子化学合成了VP1中编码133～158位氨基酸及VP4中编码20～34位氨基酸这两个抗原表位基因，将其组成133～158-20～34-133～158串联结构，在其5′端加上鼠Igκ链信号肽序列，在翻译调节区加上增强表达的Kozak序列，同时在133～158-20～34-133～158串联结构3'端终止密码子下游加上CpG免疫刺激序列，将这些片段连接后，克隆到真核表达载体pHook-2上，构建成功了DNA疫苗重组表达载体pAS1-E。

It is only man that has arrogated to himself that privilege.

上天从来没有刻意去挑选过某个家庭或某个个人，或者偏爱某人、伤害某人，总是有些&首脑家庭&试图使人们相信上天有所偏私，总是有些人自称为是得到了上天所赋予的特权。

Zhu Wei makes no bones about his love of gongbi (fine-lined traditional style) painting. In his work, the mode of gongbi as a form of expression serves to bring out the idea and atmosphere of his imagery. His use of line, colour and texture shows continuity of the traditional practice, but because his conceptualisation originates from reality, a flavour of contemporary time is palpable in his works.

朱伟毫不隐晦自己对传统工笔表现方式的偏爱，不过，在他的作品里，方式完全从属于画面所体现的观念及气氛所需，例如他在技法上——诸如线条、色彩、机理等等，皆与传统方式的延续有关，但由于他的观念是从现实中生长，因此他作品中强烈的当代气息扑面而来。

That is the excuse for her to be partial?

那是她偏爱的理由吗？

The difference between him and the others forced me to be less partial.

也这是因为这种区别，让我在他和其他孩子之间没有偏爱任何人。

Waits for your dependence to be partial to you .

等迩的依赖，对你偏爱。

There is no sure defence against bad ideas, but one place to start is with a well-educated and sceptical citizenry that is free to listen to the notions of the intellectuals but is not in thrall to them—and, yes, may prefer the sports channel instead.

有害思想防不胜防，重要的是要拥有一群接受良好教育且坚持怀疑精神的民众，他们可以自由聆听不同的声音而又不被它们束缚，并且也许能对体育频道有所偏爱。

He use of music box sounds and slightly airier synths in "Tears -bygone days-" gave it a different vibe from a track that I thought was one of the better pieces in Memories Off.

ears -bygone days-中使用了八音盒和轻快的电子乐，让我对这首前作中就比较偏爱的曲目又有了耳目一新的感觉。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。