假细菌的

Objective:To investigate the destructive effect of erythromycin on biofilm of pseudomonas aeruginosa in vitro.

目的：建立铜绿假单胞菌生物被膜的体外模型，研究红霉素对细菌BF的影响及对左氧氟沙星的增效作用。

Though CopRS/CopABCD homologues are found to exist in Pseudomonas fluorescens, the potential role of this system in P. fluorescens has not been investigated. In this study a genetic cluster, consisting of copR, S, C, and D but lacking copAB, was identified in a pathogenic P.

CopRS/CopABCD是细菌用以维持铜内环境稳定的一个系统，虽然已在荧光假单胞菌中发现了CopRS/CopABCD系统的同源物，但其潜在的功能还未知。

Results showed that strain SY21 was Acinetobacter sp., strain SY22 Neisseria sp., strain SY23 Plesiomonas sp., strain SY24 Xanthomonas sp., strain SY42 Azotobacter sp., strain SY43 Flavobacterium sp., and strain SY44 Pseudomonas.sp.. Removal efficiency of the bacteria to TPH reached about 80% after 8 days' inoculation, which is higher than that of bacteria reported.

菌株鉴定结果表明，SY21为不动细菌属，SY22为奈瑟氏球菌属，SY23为邻单胞菌属、SY24为黄单胞菌属、SY42为动胶菌属、SY43为黄杆菌属、SY44为假单胞菌属。7株菌的降油试验结果表明，降解8d后，加菌试样的石油烃降解率均达到80%左右，7株菌的石油烃降解速率高于目前已有的报道。

TBA analysis showed 0.01% of TP had evident antioxidation on lipids from fish meat, and TBA increased with the TP concentration accretion presenting an unlinear relation. The effects of antioxidation of 0.03% and 0.07% of TP were close, thus, the best choice is 0.03% in practical application.5. 93 bacteria strains were isolated and categorized into several spoilage bacteria classes: Aeromonas, Vibrio, Pseudomonas, Enterobacteriaceae, Flavobacterium, Acinetobacter, Photobacteriaceae, Brevibacterium, and Micrococcus. In the early stage of storage (1～3 days), Gram-positive bacteria were the major strains and accounted for 70%, most of them belonged to Brevibacterium and Micrococcus.

TBA试验结果显示，添加00.1%以上茶多酚对草鱼脂肪都有明显的抗氧化作用，随浓度增加TBA值增加，但不呈线性关系，0.03%～0.07%抗氧化效果比较接近，从经济、有效的角度考虑在实际应用中可采用0.03%的茶多酚。5、从草鱼中分离出93株菌株，鉴定出以下几类主要腐败细菌：气单胞菌属、弧菌属、假单胞菌属、肠道杆菌科、黄杆菌属、邻单胞菌属、发光杆菌属、产碱杆菌属、短杆菌属、微球菌属、不动杆菌属、无色菌属。

93 Target gene were detected by Tem-PCR from 75 specimen, they were: Hemophilus influenzae 40, Streptococcus penumoniae 36, Acinetobacter baumannii 10, Pseudomonas aeruginosa 4, Staphylococcus aureus 3, the other 9 kinds of bacterium including Escherichia coli、Klebsiella pneumoniae and Enterobactor cloacae were not detected by Tem-PCR, the positive rate of Tem-PCR was 39.9%(75/188).(3) For the 14 kinds of bacterium designed by Tem-PCR, compared with the culture, the sensitivity、specificity and coincidence of Tem-PCR is 51.0%, 68.0%, 58.3% respectively.

2经Tem-PCR技术扩增后，188例标本在Luminex100多功能悬浮点阵仪中有75例呈阳性，共检测出93株病原菌的靶基因，分别是流感嗜血杆菌40株，肺炎链球菌36株，鲍曼氏不动杆菌10株，铜绿假单胞菌4株，金黄色葡萄球菌3株，另外9种Tem-PCR已设计的细菌包括肺炎克雷伯菌、大肠杆菌、阴沟肠杆菌等均未检出，Tem-PCR的阳性率是39.9%(75/188)。

Methods From Feb 2006 to Jun 2006,188 hospitalized children in Shenzhen children s hospital, were collected deep tracheal aspirate at the time of hospitalization. The respiratory tract secretions were immediately sent for bacterial culture with 3 kinds of medium:ordinary medium, Hemophilus influenzae selective medium, Streptococcus penumoniae selective medium. Then we extracted the total nucleic acids from secretions, and detected Mycoplasma pneumoniae by single fluorescent quantitation PCR. Simultaneously, 14 respiratory tract pathogenic bacterium and Mycoplasma pneumoniae were detected by Target Enriched multiplex PCR. Amplification products were identified by the Luminex100 suspension array.

确诊为社区获得性肺炎的患儿188例，在入院当天采集深部呼吸道吸引物，用普通培养基和肺炎链球菌、流感嗜血杆菌选择性培养基进行细菌培养，然后提取深部呼吸道吸引物中病原体的DNA，采用荧光定量单PCR的方法检测肺炎支原体，并对同一标本采用靶序列富集多重PCR技术同时扩增肺炎链球菌、流感嗜血杆菌、金黄色葡萄球菌、肺炎克雷伯菌、大肠杆菌、嗜肺军团菌、铜绿假单胞菌、鲍曼氏不动杆菌、脑膜炎奈瑟氏菌、阴沟肠杆菌、奇异变形杆菌、化脓链球菌、粪肠球菌及屎肠球菌14种呼吸道病原菌和肺炎支原体的靶基因，扩增产物用Luminex100多功能悬浮点阵仪检测。

20 Centuries 60 time, american Gristina put forward, all biology material are embedded inside body hind, its surface all can form a kind of biomembrane, bacterial criterion is many in this kind of biomembrane progenitive, the many mucilage outside producing a cell is qualitative (Extracellular Slime Substance, ESS), it is the easy hair sex that causes false body infection and the main factor that study a gender hard.

概要： 20世纪60年代，美国Gristina等提出，所有的生物材料植入体内后，其表面均能形成一种生物膜，细菌则在这种生物膜中大量繁殖，并产生细胞外多粘质物质(Extracellular Slime Substance，ESS)，是导致假体感染的易发性和难治性的重要因素。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新