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Using Pseudomonas syringae and Pantoea ananas , the dominant ice nucleation active bacteria strains from Yunnan province, as receptors of genetic engineering recombination, and Escherichia coli S17/pZJ25∶∶Tn5 as donator. By transposon Tn5 induced mutants method, the Tn5 was inserted and integrated into the genome of wild type INA + bacteria. INA + mutant as INA - when the linear continuous of INA + gene destroyed.

以云南省冰核细菌的优势种类丁香假单胞菌Pseudomonassyringae和菠萝泛菌Pantoeaananas为重组基因工程菌的受体菌,采用Tn5转座子诱变技术,以大肠杆菌EscherichiacoliS17/pZJ2 5∶∶Tn5作为转座子诱变的供体菌,使Tn5插入并整合到冰核细菌基因组中,使冰核基因的线性连续性被破坏,失去冰核活性。

Results The results showed that the average concentration of the bacteria, fungi and total microbes in the outdoor environment were 5.04×10^4, 3.75×10^3 and 5.41×10^4 cfu/m^3 respectively and the fungi accounted for 6.92% of the total. The preliminary identification showed that the dominant bacteria mainly were Micrococcus, Bacillus, Pseudomonas and Staphylococcus and the average percentage were 37.3%, 21.0%, 10.3% and 9.5% respectively. Of the five fungi the dominant ones were Aspergillum, Penicillium, Mucor and Rhizopu. The average percentage were 42.0%, 36.0%, 4.4% and 4.2% respectively. Conclusion The outdoor air of Hefei has been polluted by airborne microbes.

结果 合肥城区空气细菌总数均值为5.04×10^4cfu/立方公尺,真菌总数均值为3.75×10^3cfu/立方公尺,合计为5.41×10^4 cfu/立方公尺,其中真菌占6.92%;对不同采样点的空气细菌和真菌进行了初步鉴定,优势细菌属为微球菌属、芽孢杆菌属、假单胞菌属和葡萄球菌属,分别占总细菌的37.3%,21.0%,10.3%,9.5%;真菌共检出5属,其中优势菌属为曲霉属、青霉属、毛霉属、根霉属,分别占总真菌的42.0%,36.0 %,4.4%,4.2%。

Besivance is indicated for the treatment of bacterial conjunctivitis in patients aged ≥1 year whose infection is caused by susceptible isolates of the following bacteria: CDC coryneform group G, Corynebacterium pseudodiphtheriticum, Corynebacterium striatum, Haemophilus influenzae, Moraxella lacunata, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus hominis, Staphylococcus lugdunensis, Streptococcus mitis group, Streptococcus oralis, Streptococcus pneumoniae, and Streptococcus salivarius.

Besivance适用于治疗年龄≥1 岁、由下列细菌的致病分离物所致的细菌性结膜炎患者:CDC棒状杆菌G群,假白喉棒杆菌,纹带棒杆菌,流感嗜血杆菌,腔隙莫拉菌,金黄色葡萄球菌,表皮葡萄球菌,人葡萄球菌,路邓葡萄球菌,缓症链球菌群,口腔链球菌,肺炎链球菌以及唾液链球菌。

Results The testing results showed that this method with 100% specificity and sensitivity in 300 positive and 100 negative samples of mammalian sera respectively. However, the positive serum samples of Francisella tularensis, yersinia pseudotuberculosis and yersinia enterocolitis could not be deceted by dipstick of ICA.

结果 间接血凝法检测阳性300份血清标本,胶体金法检测全部为阳性,间接血凝法检测阴性100份血清标本,胶体金法检测全部为阴性,两种方法符合率100%;并且鼠疫胶体金法快速诊断盒不与假结核耶尔森氏菌等相关细菌的免疫血清发生交叉反应。

The effects of removal of organic nitrogen by different ammonibacterium were compared and the scale to the quantity of organic nitrogen which consumed by ammonibacterium is the quantity of ammoniacal nitrogen which is produced.

结果表明,芽孢杆菌属、假单胞菌属为人工湿地中氨化细菌的优势菌属;氨化细菌-1、氨化细菌-2及氨化细菌-5对有机氮的去除效果相对较好,分别达到46.2%、49.4%和52.6%。

The antimicrobial test and researched was done on wide distillation of different solvents on Terpenoid, polarity separation of ethanol distillable substance, thin layer chromatography of different polarities and distillation of amylase. The results showed that the active substance distilled by 95% ethanol and V methanol: toluene=3:1 had stronger antimicrobial activity on Agaricus blazei Murrill. The antimicrobial activity of ethyl acetate was stronger than wide distilling substance with ethanol, but the water part has not antimicrobial effect. Two straps of ethyl acetate phase(Rf0.57、Rf0.63)(0.1g/ml)had stronger antimicrobial activity on Agaricus blazei Murrill, the antimicrobial zone on Pseudomonas was 19.3mm. The bacteriostatic activity of chromatography strap of water phase was rather weaker, the water solution (0.5g/g) of amylase had better effect on pathogenic fungus of Agaricus blazei Murrill, its antimicrobial zone on Aspergillus niger, Citrinin, Trichoderma viride were 10.8 mm, 7.9 mm, 5.3 mm.

通过对日本凹顶藻以不同溶剂的粗提、乙醇提取物的极性分离、不同极性的薄层层析和多糖的提取,进行抗菌试验的研究,发现95%乙醇和V甲醇:甲苯为3∶1的提取的活性物质具有较强的抗姬松茸的病原细菌活性,乙酸乙酯相对病原细菌的抑菌强于乙醇的粗提物,而水相基本无抑菌效果,乙酸乙酯相的两个带(Rf0.57、Rf0.63)浓度为0.1g/ml具有较强抗姬松茸病原细菌的活性,对假单孢杆菌抑菌圈达到19.3mm,水相层析带的抑菌活性较弱,多糖的粗提物配成0.25g/g的水溶液对姬松茸病原真菌有较好的抑菌效果对黑曲霉、桔青霉和绿色木霉的抑菌圈分别达到10.8 mm、7.9 mm、5.3 mm。

Many pathogens of animal and plant, such as Erwinia cawtovora pv.carotovora and Pseudomonas aeruginosa, producing some kinds of virulence factors before attacking the host, and most of those virulence factors was modulated by the quorum-sensing system which relys on the secret of some small quorum sensing signals.

很多动植物的病原细菌,包括胡萝卜软腐欧文氏菌和铜绿假单胞菌等在侵染动植物有机体的过程中,首先利用细菌的群体感应机制诱导致病因子的表达,在致病因子的作用下侵染宿主。

Bacillus subtilis str. B5423, Pseudomonas fluorescens str. Pf7-14, nonfluorescent pseudomonads str. P13 are antagonistic to rice sheath blight. Resistance to antibiotic is the character that was chosen to use to mark the strains in order to monitor the population dynamics of the strain on the surface of rice plant and to elucidate the mechanism of suppressing the diseases.

中文题名拮抗细菌的生态学及其对水稻纹枯病的抑制作用副题名外文题名 Ecology of biological control agents and their suppressive effect on rice sheath blight 论文作者李湘民导师许志刚教授苗东华博士学科专业植物病理学研究领域\研究方向植物病害生物防治学位级别博士学位授予单位南京农业大学学位授予日期2002 论文页码总数116页关键词纹枯病拮抗细菌水稻馆藏号BSLW /2003 /S435 /19 Bacillus subtilis B5423,Pseudomonas fluorescens Pf7-14,和非荧光假单胞肝菌Pseudomonas sp.P13是对水稻纹枯病菌有拮抗作用的三支细菌菌株。

The pathologic changes of fungal diseases in alimentary canal were tissue necrosis, ulcer formation and pseudomembranous inflammation.

其病变为消化道组织坏死、溃疡形成及假膜性炎症;炎细胞浸润不明显,且以单核细胞浸润为主;假膜性炎中有较多的中性粒细胞渗出,可能与某些细菌的混合感染有关系。

By suspending cell culture from the callus of P. thugbergii and by fluorescent observation, a new method, which is easy and sensitive to observe cell activity and to test the phytotoxicity of various bacteria, was used. This method can be utilized to study the bacterial toxins. Cultured filtrates of some strains of the nematode-carrying bacteria were bioassayed for their toxicity to suspending cultured cells from P. thugbergii callus. Strong toxic effects were observed by the filtrates of Pseudomonas spp., P. fluorescence, and Sphingomonas spp., these bacteria species are coincide with those in the early stage of pine wilt disease.

本试验使用了黑松愈伤组织的细胞悬浮培养方法,用荧光方法观察各种细菌对植物细胞的毒性测定,这种方法具对细胞的活性观察容易、灵敏度高等特点,证明可用于细菌的毒素研究;对松材线虫携带的细菌的部分菌株,用其培养滤液对黑松愈伤组织的悬浮培养细胞的生物测定初步证明,松材线虫携带的细菌中以假单胞菌、荧光假单胞菌和少动鞘氨醇单胞菌的滤液对黑松细胞具有较强的毒性,这与松树发病早期出现的细菌种类相一致。

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But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊!不用提了。提到肉,真是糟透了。

Tristan, I have nowhere to send this letter and no reason to believe you wish to receive it.

Tristan ,我不知道把这信寄到哪里,也不知道你是否想收到它。