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The 16S rDNA sequence of the 4 strains were aligned with the 16S rDNA sequences of other species in genus Burkholderia,and the polygenetic tree was produced by using a Clustal V and treeview software.The results showed that P.cocovenenans subsp.farinofermentans was in a high homology with Burkholderia gladioli and Burkholderia cocovenenans, and they formed an independent phylogenetic clustal of genus Burkholderia.Key words: P.cocovenenans subsp.farinofermentans,16S rDNA sequcence,phylogeny

树状图及不同菌种之间的同源性比值:椰酵假单胞菌(P.cocovenenans subsp.farinofermentans)与唐菖蒲伯克霍尔德氏菌、椰毒伯克霍尔德氏菌的亲缘关系非常近,与其它菌种的亲缘关系相对较远,椰酵假单胞菌、唐菖蒲伯克霍尔德氏菌和椰毒伯克霍尔德氏菌可归属于一个独立的系统发育系。

False positive were lowered to an acceptable rate by this protocol, therefore, a big amount of work on fingerprint to get rid of false positive clones can be avoided. When whole BAC clone containing one locus of a multi-loci probe were applied for library screening, no matter homogenic BAC or heterogenic BAC, site-specificity is higher than short DNA fragment probes.

对于无法通过小基因组填补间隙的区段,可以本物种BAC克隆直接进行染色体步移,采用抑制重复顺序杂交法能够有效抑制大量存在于植物基因组中的重复顺序的干扰,把假阳性率降低到可以接受的范围,从而避免了用大量指纹分析剔除假阳性克隆。

For example, we expect test methods to identify the presence of organisms such as Pseudomonas cepacia or other Pseudomonas species that may be objectional or present a hazard to the user.

例如,我们期望用的方法能够识别洋葱假单胞菌或者其他假单胞菌属的微生物这样的控制菌或者给使用者带来危害的微生物。

The results indicate that the presence of GSH increased the expression of exoY、exoS、phzA1 and gacA,while no difference was observed with others virulence factor genes tested.PAO1 produced markedly less pyocyanin than the wild type strain PAOIP<0.01Treating with GSH, the PCN production of PAOI(△gshB was increased from 0.47μg/mL to 1.73μg/mL (P<0.01),the PCN production of PAO1 increased from 1.3μg/mL to 1.9μg/mL(P<0.05),this result indicates that GSH upregulates PCN production.

结果发现,GSH对启动子库中exoS、exoY、phzA1和gacA等致病性因子的表达有促进作用,对其余测试过的致病性因子则没有明显影响;铜绿假单胞菌分泌的重要致病因子绿脓菌素(pyocyanin,PCN)的含量,在PAO1中远低于PAO1P<0.01加入GSH后,PCN平均含量在PAO1(△gshB中从0.47μg/mL提高到1.73μg/mL,差异十分显著(P<0.01),在PAO1中从1.3μg/mL提高到1.9μg/mL,差异显著(P<0.05),表明GSH对铜绿假单胞菌生产PCN有促进作用。

A important causation of morality anomie of society is that people rifely lack emotion of disgrace and this has inevitable relation with that moral education of high and elementary school chronically neglect to foster the students' emotion of disgrace.

产业界造假;商业界售假;卫生界收红包、开假处方;学界乱收费、发假文凭;司法界收礼受贿、贪赃枉法,如此等等,可谓应有尽有。社会道德失范、人们的行为失德,原因是多方面的。从失德的行为主体方面考察,耻感的缺失是其中的一个重要原因。

Results In static phantom studies, the percent error of mean activity obtained by Carson's method was reduced to about 9 %, and it is less than that of image-derived method using maximum likelihood expectation maximization (about 14 %). In simulated dynamic phantom studies, the vessel component could be extracted using ICA in image and sinogram domain. In sinogram domain, the six components extracted by ICA could be obtained quickly.

在静态物理假体实验结果来看,使用Carson's method计算出感兴趣区域活性的百分比误差( 9%)比直接从影像取得活性的百分比误差( 14%)来得小;从数位模拟动态假体实验结果来看,独立成份分析法运用在影像空间或是弦波图空间上,都可以将血管分离出来,但从弦波图空间上得到各个独立成份相对於影像空间就快得多;imageICA和sinogramICA计算出的输入函数的标准化均方根误差分别是0.57和0.28。

OBJECTIVE, To study the direction, route and num- ber of SLCs and their relationship with SLN after subareolar injection of methylene blue and technetium 99m sulfur colloid (^99m Tc-SC), and to explore the mechanism of sentinel lymph node biopsies produce false negative results in breast cancer patients and the method to improve the detectable rate, METHODS: ^99m Tc-SC was injected preoperatively, and methylene blue was injected by the same subareo-lar route just after anesthetic induction, and then SLNB was carried out according to SLCs in 93 breast cancer patients.

目的:通过乳晕下联合注射^99m Tc-SC和亚甲蓝,研究前哨淋巴通道的行走方向、途径、数量及其与前哨淋巴结之间的关系,以探讨乳腺癌SLNB出现假阴性的机制,并提出提高其检出率、减少假阴性的方法。

At an average of 30 months follow- up for cemented hip joint replacement and uncemented HJR, a retrospective analysis was conducted on those patients with or without osteoporosis in femur.

方法对99例(99髋)术前有股骨干骺段骨密度测定值的人工髋关节置换患者进行了平均30个月的随访调查,回顾性分析骨水泥假体和非骨水泥假体在治疗正常骨密度患者和骨质疏松患者上的近期疗效的差别,采用Harris评分、大腿处疼痛、患者满意度三个方面进行比较。

San Baw (29 June 1922 - 7 December 1984), pioneered the use of ivory hip prostheses to replace ununited fractures of the neck of femur ('hip bones'), when he first used an ivory prosthesis to replace the fractured hip bone of an 83 year old Burmese Buddhist nun, Daw Punya.

缅甸于1960年整形外科医生,博士旧金山的BAW ( 1922年6月29日- 1984年12月7日),开创了利用象牙髋关节假体,以取代不愈合骨折的股骨颈,当他第一次使用了象牙假体,以取代髋骨骨折的83岁的缅甸尼,昂

The results also reveal that there are no subsequent memory effects in pseudo-character. It seems that processing of pseudo-character did not activate preexisted mental representation in memory.

实验结果还显示,在假字中没有发现显著的相继记忆效应,可能在假字加工过程中没有明确通达记忆中预存的心理表征,而这种心理表征的通达是相继记忆效应产生的前提。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。