假整合的

Using Pseudomonas syringae and Pantoea ananas , the dominant ice nucleation active bacteria strains from Yunnan province, as receptors of genetic engineering recombination, and Escherichia coli S17/pZJ25∶∶Tn5 as donator. By transposon Tn5 induced mutants method, the Tn5 was inserted and integrated into the genome of wild type INA + bacteria. INA + mutant as INA - when the linear continuous of INA + gene destroyed.

以云南省冰核细菌的优势种类丁香假单胞菌Pseudomonassyringae和菠萝泛菌Pantoeaananas为重组基因工程菌的受体菌，采用Tn5转座子诱变技术，以大肠杆菌EscherichiacoliS17/pZJ2 5∶∶Tn5作为转座子诱变的供体菌，使Tn5插入并整合到冰核细菌基因组中，使冰核基因的线性连续性被破坏，失去冰核活性。

The second half inspects SciTech from the four angles of the wholeness of the system of human nature: from the angle of self, as the production of making use of objects by self, SciTech can be in favor of self or dissever self, which, the author thinks, is little helpful to the consolidation and transcendence of self; from the angle of individuality, for its aim is to eliminate individuality and to pursue commonness, there is distinct conflict between SciTech and individuality; from the angle of valueness, only have been the means of affirming and realizing the value of human nature and the purpose of personality, can SciTech have internal legitimacy; from the angle of sublimatity, human's right and rational needs make SciTech impel development of human nature healthfully, lust which is the distortion of needs make the trap, and technologizing the human nature will never be the way and aim of sublimating human nature.

下部分从人性系统整体性的四个角度考察；从自我看，科技作为自我"假于外物"的产物而推动或肢解自我，对自我的整合和提升少有实质性作用；从个性看，它是销匿个性而寻求共性的结果，与个性之间矛盾突出；从价值性看，它只有成为确证和实现人性价值和人格目标的手段，才具有内在合法性；从提升性看，人的正当合理的需要使科技推动人性健康发展，欲望对需要的扭曲造成陷阱，使人性技术化不是人性提升的途径和目标。

We hypothesize that adequate nurturing and the absence of intense early stress permits our brains to develop in a manner that is less aggressive and more emotionally stable, social, empathic and hemispherically integrated.

我们的假说是，充分的照顾以及免于早期强烈的压力，可让我们的大脑发育成不那麽具有攻击性、绪较稳定、易相处、同理心，以及整合良好的左右半球。

Many ultrabasic bodies in the orogenic association occur along strike faults, which gives a simulated concordant relationship

与造山运动共生的许多超基性岩体沿着走向断层出露，表现出假的整合关系。

One was the pqsA' positive expression plasmid constructed by cloning the pqsA'-lacZ fusion digested from pYHP441 into miniCTX-1, whose sequence was then integrated into wild type PAO-1 chromosome by biparental mating procedure. The other was pqsA-E operon knock-out plasmid whose sequence between pqsD and pqsE operon was inserted by tetracycline cassette by the site specific insertion mutagenesis strategy and the mutant constructed by biparental mating of S17-1 that harbored the plasmid and pqsA' positive expression mutant.

一种是酶切质粒pYHP441获得pqsA'-lacZ片段后，亚克隆入质粒miniCTX-1中，构建成PqsA'的阳性表达质粒，随后将构建的质粒，通过双亲交配过程整合入野生型铜绿假单胞菌株PAO-1染色体组中；另一种是通过点特异插入诱变策略，将四环素基因盒插入启动子pqsD和pqsE之间，构建的阴性质粒转化入大肠杆菌S17-1株后，和上述pqsA'阳性表达突变株进行双亲交配过程。

Here is the summary of currently research of metallo-β-lactamase genes,carried by integron in Pseudomonas aeruginosaincluding IMP,VIM,SPM,and GIM.

本文就铜绿假单胞菌中整合子携带的金属β-内酰胺酶基因IMP、VIM、SPM和GIM的研究进展作一综述。

By microinjection techniques on germ nucleus of zygotes hMR-1 gene were injected into zygotes of CD-1?∕BR mice and transplanted into the oviducts of pseudopregnancy female mice.

ICRBR小鼠受精卵中，并移植到假孕受体鼠的输卵管内，然后应用PCR、Western Blot杂交技术分析子代鼠中基因的整合及表达情况。

Lines transformed by particle bombardment have been discussed. The selectable marker bar gene cassette that integrated with the rice genome had multiple copies and showed complex segregation behavsensitive plants in their progenies. In five out of ten original transgenic lines, bar gene can be stably transmitted as a dominant gene to self-pollinated T2 progeny.

结果发现：作为筛选标记的bar基因表达盒在水稻基因组中多拷贝整合，遗传分离行为复杂，还出现了Basta抗感分离比在35∶1～144∶1之间的&假纯合体&，但50﹪转基因株系中(5/10)bar基因可作为一个显性基因按孟德尔方式稳定遗传至自交T2代。

Lines transformed by particle bombardment have been discussed. The selectable marker bar gene cassette that integrated with the rice genome had multiple copies and showed complex segregation behaviors including the presence of 'false homozygotes', with abnormal segregation ratios ranging from 35:1 to 144:1 (Basta-resistant: sensitive plants) in their progenies. In five out of ten original transgenic lines, bar gene can be stably transmitted as a dominant gene to self-pollinated T2 progeny.

结果发现：作为筛选标记的bar基因表达盒在水稻基因组中多拷贝整合，遗传分离行为复杂，还出现了Basta抗感分离比在35：1～144：1之间的&假纯合体&，但50%转基因株系中（5/10）bar 基因可作为一个显性基因按孟德尔方式稳定遗传至自交T2代。

The sensitivity rate of Polymyxin B、aminoglycoside antibiotics and ciprofloxacin are higher than other antibiotics.

探讨整合子携带的耐药基因盒是否介导铜绿假单胞菌对IPM的耐药。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。