假孕的

MPGES-2 immunostaining in ampulla was slightly weaker than that in infundibulum. During pseudopregnancy, the level of mPGES-2 protein in infundibulum was a little weaker than that during early pregnancy, and remained a strong level at all stages.

假孕过程中，mPGES-2 蛋白在输卵管伞部的免疫染色强度稍弱于早期妊娠的输卵管伞部，在各时期的免疫染色均较强，没有明显差异。

Nuclear translocation of tyrosine-phosphorylated STAT3 was also not detected in the uteri on day 4 of pseudopregnancy and under delayed implantation.

在假孕第4天的子宫和延迟着床的子宫中未发现酪氨酸磷酸化STAT3的核转位现象。

In the uterus on day 5 of pseudopregnancy or under delayed implantation,the expression signal of CD63 was hardly to be detected,but there was a specific expression after the activation of delayed implantation.

在假孕和延迟着床的子宫中，检测不到CD63基因的表达，而在延迟激活子宫的着床位点，CD63基因的表达特异性增强，其表达模式与正常妊娠第5天着床位点处类似。

The aim of this study was to examine caveolin-1 expression and regulation in mouse uterus during early pregnancy, pseudopregnancy, delayed implantation, artificial decidualizarjon and hormonal treatment by in situ hybridization and immunohistochemistry.

本实验以小鼠为材料，利用原位杂交和免疫组化方法，首次检测了小窝蛋白-1基因在小鼠早期妊娠子宫中的表达，并利用假孕、延迟着床、人工蜕膜化及激素处理等模型研究小窝蛋白-1基因在子宫中的表达与调节。

The aim of this study was to examine cPLA2α gene expression and regulation in mouse uterus during early pregnancy, pseudopregnancy, delayed implantation, artificial decidualization and hormonal treatment by in situ hybridization and immunohistochemistry.cPLA2α immunostaining was detected in the luminal epithelium and glandular epithelium of mouse uterus from days 1 to 5 of pregnancy.

本实验以小鼠为材料，利用原位杂交和免疫组化方法，检测cPLA2α基因在小鼠早期妊娠子宫中的表达，并利用假孕、延迟着床、人工蜕膜化及激素处理等模型研究cPLA2α基因在子宫中的表达与调节。

The aim of this study was to investigate PGT, 15-PGDH and CBR1 expression in themouse uterus during early pregnancy and their regulation in related models of pseudopregnancy,delayed implantation, artificial decidualization, steroid hormone treatment by in situ hybridization,RT-PCR and immunohistochemistry.

本研究利用原位杂交、免疫组织化学和RT-PCR等方法，检测了PG转运载体PGT、代谢酶15-PGDH和CBR1在小鼠早期妊娠子宫中的表达，并利用假孕、延迟着床及激活、人工蜕膜化、性腺类固醇激素处理等模型，研究了它们在小鼠子宫中的表达与调节。

Result: In total, 3264 fertilizated eggs were injected,1779 of which developedinto 2-cell embryos after in vitro cultured.The survival rate was 54.50%.1397survived embryos were reimplanted into the oviducts of psudopregnant kunbaifemale mice.After reimplantation,16 female mice were pregnant and 85 pups wereborn. PCR analysis indicated 11 out of 85 pups weretransgenic.SouthernBlotting detection showed 1 out of 11 was transgenic.

结果：共注射了3264个受精卵，继续培养，有1779个胚胎发育至2-细胞，存活率为54.50%，将1397个存活的胚胎移植到71只假孕昆白母鼠的输卵管内，共有16只移植母鼠怀孕，得到85只仔鼠，PCR检测表明11只为转基因阳性小鼠，对其中的部分小鼠进一步进行Southern Blotting检测显示，1只为转基因阳性鼠。

T lymphocyte percentages of peripheral blood, lymph node, spleen, thymus and bone marrow of maters of interspecific, intraspecific embryo implantation and pseudopregnant mice at corresponding time were measured using fluorescence labeled monoclonal antibody and flow cytometry.

利用荧光标记的单克隆抗体染色结合流式细胞术，检测种间、同种胚胎移植以及同时期假孕母体外周血、淋巴结、脾脏、胸腺、骨髓中T淋巴细胞的百分率。

In situ hybridization using biotinylated POMC cRNA probe made it possible to localize POMC mRNA in the rat ovary. POMC mRNA predominantly existed in corpora lutea of diesturs, pregnant and pseudopregnant rat ovaries. POMC containing cells scattered in the ovarian intertitium were seen. The amount of POMC mRNA in granulosa cells was negligible.

采用生物素标记的POMCcRNA作为探针，进行原位杂文，研究POMCmRNA在大鼠卵巢中的分布，实验表明，POMCmRNA主要分布于间期，妊娠和假孕大鼠卵巢黄体中，间质细胞也可见少量POMCmRNA，而卵泡颗粒细胞中POMCmRNA极少。

By microinjection techniques on germ nucleus of zygotes hMR-1 gene were injected into zygotes of CD-1?∕BR mice and transplanted into the oviducts of pseudopregnancy female mice.

ICRBR小鼠受精卵中，并移植到假孕受体鼠的输卵管内，然后应用PCR、Western Blot杂交技术分析子代鼠中基因的整合及表达情况。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。