假孕的

Intrauterine phantom pregnancy bursa is one sign of ultrasonography in diagnosis of ectopic pregnancy.

宫内无孕囊是超声诊断异位妊娠的征象之一，超声鉴别宫内假孕囊是避免早期异位妊娠误诊的关键。

Methods: The fetuses of interfamily pregnancy day 4, day 8 and day 12 were acted as experimental group, while the ones of intraspecific pregnancy day 4, day 8 and day 12 of embryo transfer and the uteri of pseudopregnant mice of corresponding time were employed as positive and negative groups respectively.

以科间妊娠第4天、第8天、第12天的孕体为实验组，小鼠同种移植妊娠第4天、第8天、第12天的子宫孕体和相应时间假孕小鼠的子宫为阳性和阴性对照。

MethodsEarly embryos at different stages and uterus of the non-pregnant, and pregnant uterus of 1 to 8 days pseudopregnancy in mice were obtained.

方法取小鼠早期胚胎、动情期未孕、真孕及假孕1～8d小鼠子宫，利用免疫组织化学染色及图像分析法，检测p38MAPK在不同发育阶段早胚及围植入期子宫内膜中的表达和变化规律。

There was a low level of basigin mRNA expression in the luminal epithelium on days 1 to 8 of pseudopregnancy and glandular epithelium on days 2 to 6 of pseudopregnancy.

Basigin mRNA在假孕大鼠子宫第1-8天腔上皮及第2-6天腺上皮持续表达，而basigin蛋白质则在假孕第1-2天的腔上皮及第2-6天腺上皮表达。

Methods Male mice were killed by cervical vertebrae dislocation, the caudae epididymides were removed and squeezed to HTF medium, then add oocyte-cumulus complexes, after IVF 22~24 h, wash the embryos and selected the two-cell embryos to 0.5 d pseudopregnant ICR mice.

对于需要净化的小鼠的雄鼠，采集附睾的精子，放入HTF溶液中获能，然后加入经过超排的卵团，体外受精。20~22 h后，挑选形态正常的二细胞胚胎，在净化实验室，移植给假孕的SPF级ICR母鼠，待产仔。

Methods: The fetuses of interfamily pregnancy day 4, day 8 and day 12 were acted as experimental group, while the ones of intraspcific pregnancy day 4, day 8 and day 12 of embryo transfer and the uteri of pseudopregnant mice of corresponding time were employed as positive and negative groups respectively.

以科间妊娠D4、D8、D12的孕体为实验组，小鼠同种移植妊娠D4、D8、D12的子宫孕体和相应时间假孕小鼠的子宫为阳性和阴性对照。

The healthy survivals were also transferred into the oviducts of pseudopregnant female mice. PCR was used to analyze the integration of the transgene in the genomes of mice.

选用内交系C57BL／6J种小鼠作为受精卵的供体鼠，昆明种小鼠作为假孕母鼠，将长2583bp、携带有CMV启动子和人CKLF-1基因的片段显微注射到受精卵的原核内，然后将注射后成活且健康的受精卵移植到假孕母鼠的输卵管内使其发育。

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法：利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变，使编码子249由精氨酸变成丝氨酸，然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中，将注射过的囊胚植入假孕的雌性小鼠子宫，到第14d取小鼠胚胎纤维母细胞，用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞，经测序证实细胞含有由249Arg到Ser的突变。

It was shown that: 1 MMP2 activity reached the highest level during proestrus, and was lowest during diestrus; 2 MM2 acti vity was increased from the first day of pregnancy, peaked on day 4 of pregnancy; but decreased significantly at day 7 of the pregnancy. 3 MMP2 activity was obviously higher on day 1 of pseudopregnancy than on the other days, and its activity decreased gradually, reached the very low level at day 4 of pseudopregnancy and maintained the low level until day 7; 4 MMP2 activity was decreased significantly 24 hours after injection of 17βestradiol ( 01 mg) at metaestrus, while MMP2 activity had no change after progesterone (04 mg) injection.

明胶酶A活性变化如下：1在大鼠的动情前期明胶酶A的活性最高，间情期最低；2在妊娠早期(1～7天)的卵巢中，明胶酶A的活性在妊娠第4天达到最高，第1～3天及第5、6天其活性也较高，但第7天则明显下降；3在假孕(1～7天)大鼠的卵巢中，明胶酶A的活性在假孕第1天最高，第2、3天略下降，从第4天到第7天，其活性都处于较低水平；4动情后期大鼠皮下注射雌二醇(0.1mg/只)24小时后，明胶酶A活性明显上升。

RESULTS:①A total of 178 injected and survived zygotes were replanted. Thirteen mice were born with the birth rate of 7%.

结果：①178只注射pcDNA3.1-mutant LMNA后的存活受精卵移植至假孕鼠输卵管后，出生13只子代鼠，出生率只有7%；58只注射pcDNA31 -mutant LMNA后的存活受精卵移植到假孕鼠输卵管后，在移植后12~15 d的受体母鼠子宫内收集到1只活胚和5只死胚。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。