假孕

MPGES-1 mRNA and protein were not seen in the pseudopregnant uteri.

在假孕大鼠子宫中，没有检测到mPGES-1 mRNA和蛋白的表达。

But the transfer of 2—4 cell stage parthenogenetic embryos to the pseudopregnant recipients failed to induce preqnancy in Kunming mouse.

孤雌胚具有发育良好的内细胞团。但将2—4细胞期孤雌胚移植至假孕受体未获得妊娠。

The vector was linearized and injected into 817 fertilized eggs of mice in which coagulation factor IX gene has been knocked out. The manipulated embryos were transferred into the oviducts of 45 pseudopregnant females, from which 63 offsprings were obtained.

将其线性化后，用显微注射法注射入817只凝血因子Ⅸ基因剔除小鼠受精卵雄原核，再将它们分别回输45只假孕受体母鼠的输卵管中，共产仔69只，存活63只。

Methods Purified 5-LO fragment was injected into male pronucli and the firtilized eggs were transplanted into pseudopregnant mice. PCR and Southern blot were used to detect the genotype of DNA separated from the newborn mouse tail tissues. RT-PCR and Western blot analysis were used to detect the gene transcription and expression. Results PCR and Southern blot results showed that 7 of 25 mice were transgenic mice.

通过显微注射的方法，将5-脂氧化酶基因片段(6.8 kb)导入BDF1受精卵雄原核并移植到同期受孕的假孕母鼠输卵管中，对产出仔鼠的鼠尾组织DNA进行PCR、Southern blot检测，对9、20、24号转基因小鼠分别提取腹腔细胞、骨髓细胞及脾、肾组织总RNA和蛋白，并采用RT-PCR、Western blot方法进行转录水平检测和蛋白表达检测。

MATERIALS AND METHODS: Arg to Ser mutation was introduced into the 249 position of the p53 gene by knock-in method. These ES cells with this mutation were selected according to the homologues-recombination with PCR and Southern blot. The positive ES cells without a selection marker were injected into blastocysts recovered from Hprt(superscript -/-) mice, which were derived from Hprt-deficient ES cells. The injected blastocysts then were implanted into pseudopregnant females.

材料与方法：利用基因打靶技术在小鼠胚胎干细胞p53基因249编码子中引入点突变，使编码子249由精氨酸变成丝氨酸，然后将含突变的ES细胞显微注射到Hprt小鼠囊胚中，将注射过的囊胚植入假孕的雌性小鼠子宫，到第14d取小鼠胚胎纤维母细胞，用含HAT的培养液筛选出从ES细胞分化而成的鼠EF细胞，经测序证实细胞含有由249Arg到Ser的突变。

Six hundred ova were transfered into twenty-nine pseudopregnant recipients. Fifty-five offspring were born from the ova mircroinjected with the Bcl-2 fragment expression cassette.Twelve of them were proved to be gene integration positive by PCR .Four founder mice,two male and two female ,were further confirmed to be transgenic by Southern hybridization.

用BglII+SalI+PvuI三酶切pWBS和用BglII+BamHI双酶切pWCS，分别回收并纯化3.0kb的基因片段WAP-Bcl-2-SV40polyA和3.5kb的基因片段WAP-c-myc-SV40poly，作为目的基因，通过受精卵显微注射技术，分别导入C57BL／6J雌鼠与DBA／2J雄鼠交配的F1代小鼠受精卵的雄原核中，各注射约1000枚，将存活的受精卵各600枚分别移植至29只假孕母鼠输卵管壶腹部内。

T lymphocyte percentages of peripheral blood, lymph node, spleen, thymus and bone marrow of maters of interspecific, intraspecific embryo implantation and pseudopregnant mice at corresponding time were measured using fluorescence labeled monoclonal antibody and flow cytometry.

利用荧光标记的单克隆抗体染色结合流式细胞术，检测种间、同种胚胎移植以及同时期假孕母体外周血、淋巴结、脾脏、胸腺、骨髓中T淋巴细胞的百分率。

After being transplanted into uterus of synchronously pseudopregnant mouse on pregnant D4, the embryo at blastocyst stage successfully implanted and normally developed onto term in the recipient uterus.

这些胚胎被移植到同步假孕小鼠子宫内后可以着床并发育到分娩。

In situ hybridization using biotinylated POMC cRNA probe made it possible to localize POMC mRNA in the rat ovary. POMC mRNA predominantly existed in corpora lutea of diesturs, pregnant and pseudopregnant rat ovaries. POMC containing cells scattered in the ovarian intertitium were seen. The amount of POMC mRNA in granulosa cells was negligible.

采用生物素标记的POMCcRNA作为探针，进行原位杂文，研究POMCmRNA在大鼠卵巢中的分布，实验表明，POMCmRNA主要分布于间期，妊娠和假孕大鼠卵巢黄体中，间质细胞也可见少量POMCmRNA，而卵泡颗粒细胞中POMCmRNA极少。

False pregnancy, a condition in which the ***** shows symptoms of being pregnant although she has not conceived, is occasionally seen during diestrus.

假孕，即母犬没有怀孕，其表征却与怀孕非常类似的情况，经常会在发情间期中出现。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。