假孕

The aim of this study was to examine caveolin-1 expression and regulation in mouse uterus during early pregnancy, pseudopregnancy, delayed implantation, artificial decidualizarjon and hormonal treatment by in situ hybridization and immunohistochemistry.

本实验以小鼠为材料，利用原位杂交和免疫组化方法，首次检测了小窝蛋白-1基因在小鼠早期妊娠子宫中的表达，并利用假孕、延迟着床、人工蜕膜化及激素处理等模型研究小窝蛋白-1基因在子宫中的表达与调节。

The aim of this study is to examine STAT3 expression and activation in mouse uterus during early pregnancy, pseudopregnancy, delayed implantation, artificial decidualization and steroid hormonal treatments by in situ hybridization and immunohistochemistry.

本实验以小鼠为材料，利用原位杂交和免疫组化等方法，并采用假孕、延迟着床、人工蜕膜化及激素处理等模型，研究了STAT3基因在早期妊娠子宫中的表达与调节。

The aim of this study is to examine STAT3 expression and activation in mouse uterus during early pregnancy, pseudopregnancy, delayed implantation, artificial decidualization and steroid hormonal treatments by situ hybridization and immunohistochemistry.

本实验以小鼠为材料，利用原位杂交和免疫组化等方法，并采用假孕、延迟着床、人工蜕膜化及激素处理等模型，研究了STAT3基因在早期妊娠子宫中的表达与调节。

The aim of this study was to examine cPLA2α gene expression and regulation in mouse uterus during early pregnancy, pseudopregnancy, delayed implantation, artificial decidualization and hormonal treatment by in situ hybridization and immunohistochemistry.cPLA2α immunostaining was detected in the luminal epithelium and glandular epithelium of mouse uterus from days 1 to 5 of pregnancy.

本实验以小鼠为材料，利用原位杂交和免疫组化方法，检测cPLA2α基因在小鼠早期妊娠子宫中的表达，并利用假孕、延迟着床、人工蜕膜化及激素处理等模型研究cPLA2α基因在子宫中的表达与调节。

The aim of this study was to investigate PGT, 15-PGDH and CBR1 expression in themouse uterus during early pregnancy and their regulation in related models of pseudopregnancy,delayed implantation, artificial decidualization, steroid hormone treatment by in situ hybridization,RT-PCR and immunohistochemistry.

本研究利用原位杂交、免疫组织化学和RT-PCR等方法，检测了PG转运载体PGT、代谢酶15-PGDH和CBR1在小鼠早期妊娠子宫中的表达，并利用假孕、延迟着床及激活、人工蜕膜化、性腺类固醇激素处理等模型，研究了它们在小鼠子宫中的表达与调节。

Result: In total, 3264 fertilizated eggs were injected,1779 of which developedinto 2-cell embryos after in vitro cultured.The survival rate was 54.50%.1397survived embryos were reimplanted into the oviducts of psudopregnant kunbaifemale mice.After reimplantation,16 female mice were pregnant and 85 pups wereborn. PCR analysis indicated 11 out of 85 pups weretransgenic.SouthernBlotting detection showed 1 out of 11 was transgenic.

结果：共注射了3264个受精卵，继续培养，有1779个胚胎发育至2-细胞，存活率为54.50%，将1397个存活的胚胎移植到71只假孕昆白母鼠的输卵管内，共有16只移植母鼠怀孕，得到85只仔鼠，PCR检测表明11只为转基因阳性小鼠，对其中的部分小鼠进一步进行Southern Blotting检测显示，1只为转基因阳性鼠。

Methods Male mice were killed by cervical vertebrae dislocation, the caudae epididymides were removed and squeezed to HTF medium, then add oocyte-cumulus complexes, after IVF 22~24 h, wash the embryos and selected the two-cell embryos to 0.5 d pseudopregnant ICR mice.

对于需要净化的小鼠的雄鼠，采集附睾的精子，放入HTF溶液中获能，然后加入经过超排的卵团，体外受精。20~22 h后，挑选形态正常的二细胞胚胎，在净化实验室，移植给假孕的SPF级ICR母鼠，待产仔。

The aim of this study was to investigate the expression of mPGES-2 protein in mouse oviduct during the early pregnancy, pseudopregnancy, and estrous cycle by immunohistochemistry and Western blot. The regulation of mPGES-2 protein in mouse oviducts was also studied under delayed implantation, steroid hormonal treatments and oviductal ligation.

本实验主要利用早期妊娠、假孕、输卵管结扎、发情周期、激素调节以及延迟着床等实验模型，结合免疫组织化学和Western blot 的实验方法，详细研究了mPGES-2 蛋白在小鼠输卵管中的表达与调节。mPGES-2 蛋白主要定位于不同时期的伞部和壶腹部的上皮纤毛细胞中，在伞部和壶腹部肌层以及峡部和宫管连接处的上皮和肌层中未检测到mPGES-2 蛋白的表达。

Progestational agents.

假孕疗法在抑制FSH和LH和子宫内膜组织上，黄体酮和口服避孕药的作用相似。

On days 1, 2, 7 and 8 of pregnancy,no signals were detected. In the pseudopregnant mice, the expression pattern of claduin-3 was alikenormal pregnancy.

在假孕模型中，封闭蛋白-3的表达情况与正常妊娠第1-5天的表达情况基本相同，在第1和2天没有表达信号，第3和4天表达量达到最高，但在第5天的子宫内膜腔上皮中有较弱表达，腺上皮中没有信号。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。