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METHODS摘要: Molecular cloning techniques were used to construct the recombinant plasmid pLMP1p53mt containing mutant p53 gene and EB virus LMP1 gene. Linear recombinant plasmid pLMP1p53mt was transfected into mouse androgenesis of germ cells by microinjection and then survival germ cells treated were planted into fallopian tubes of artificial pregnant female mice.

方法摘要:通过分子克隆技术构建含突变型p53基因和EB病毒LMP1基因的真核表达载体pLMP1p53mt;采用显微注射法将线性化的表达载体注射至小鼠受精卵的雄性原核中,然后将注射存活的受精卵植入假孕母鼠的输卵管;取其产3 wk龄子代鼠进行PCR初选,再通过Southern杂交进一步确证;利用HE染色法观察4 mo龄转基因小鼠不同组织病理变化。

Intrauterine phantom pregnancy bursa is one sign of ultrasonography in diagnosis of ectopic pregnancy.

宫内无孕囊是超声诊断异位妊娠的征象之一,超声鉴别宫内假孕囊是避免早期异位妊娠误诊的关键。

Ulex Europeus was used as a probe to study the expression of α L Fucose in mouse uterus during early pregnancy,pseudopregnancy and estrous cycle.

应用免疫荧光技术以生物素化的荆豆素-为探针,检测其结合位点α-L-岩藻糖在小鼠妊娠 1~ 8d、假孕 1~ 6 d及发情周期子宫中的表达情况,并以摘除卵巢的小鼠为模型观察雌激素、孕酮对小鼠子宫α-L-岩藻糖表达的影响。

MPGES-2 immunostaining in ampulla was slightly weaker than that in infundibulum. During pseudopregnancy, the level of mPGES-2 protein in infundibulum was a little weaker than that during early pregnancy, and remained a strong level at all stages.

假孕过程中,mPGES-2 蛋白在输卵管伞部的免疫染色强度稍弱于早期妊娠的输卵管伞部,在各时期的免疫染色均较强,没有明显差异。

Lamin A gene cDNA lacking of 150 basyls at the end of the eleventh extron was cloned into pcDNA3.1 carrier, and the recombinant plasmid pcDNA3.1-mutant LMNA cDNA was injected into the male pronucleus of mouse zygotes, then the zytotes was replanted into the oviducts of pseudopregnant mice.

实验方法:将缺失了第11外显子末端150个碱基的LMNA基因cDNA克隆到pcDNA3.1载体,应用原核显微注射方法将线性化的pcDNA3.1-mutant LMNA注射到小鼠受精卵的雄原核,再将注射后的受精卵移植至假孕鼠的输卵管。

False pregnancy, or pseudocyesis, is a common condition in female intact dogs.

假孕,或称为假妊娠,是一种常见于未绝育母犬的状况。

Phenomenon is sometimes pseudopregnancy, or to the hospital to be a more accurate inspection

有时是假孕现象,还是到医院做个检查比较准确

CPLA2α mRNA signal could only be detected on 4, 5 and 8 of pseudopregnancy.

假孕小鼠的子宫中,在第1、4、5、8天的子宫腔上皮和腺上皮中可以检测到cPLA2α蛋白的表达,但只在第4、5、8天的子宫腔上皮和腺上皮中可以检测到cPLA2α mRNA的表达。

Early pregnancy diagnosis in cows can prevent pseudopregnancy and false heat, while early sex determination can increase proportion of cows.

奶牛早期妊娠诊断可及时消除配种后假孕、妊娠后假发情等不利因素,胎儿性别早期鉴定可提高母牛犊的出生比例。

We microinjected one of the double positive ES cell clone into the mouse blastocysts (3.5days), cultured for 4-5 hours, then implanted the blastocysts in the uteri of the pseudopregnant mice.

选择其中的一个多能胚胎干细胞显微注射到孕3.5天的小鼠囊胚内,培养4-5小时后,将囊胚移植入假孕小鼠的子宫中。

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