假孕

Methods: The fetuses of interfamily pregnancy day 4, day 8 and day 12 were acted as experimental group, while the ones of intraspecific pregnancy day 4, day 8 and day 12 of embryo transfer and the uteri of pseudopregnant mice of corresponding time were employed as positive and negative groups respectively.

以科间妊娠第4天、第8天、第12天的孕体为实验组，小鼠同种移植妊娠第4天、第8天、第12天的子宫孕体和相应时间假孕小鼠的子宫为阳性和阴性对照。

PPAR5 protein was not detected in the luminal epithelium and the glandulaxepithelium from day l to 8 of pseudopregnancy.

在假孕大鼠子宫中，PPARδmRNA第1-8天腔上皮及腺上皮上持续弱表达，而PPARδ蛋白质在假孕各期子宫中均没有检测到。

There was no detectable signal of PPARδ mRNA and protein expression in the luminal and glandular epithelium from day 1 to 8 of pseudopregnancy.

在假孕大鼠子宫中，PPARδmRNA第1-8天腔上皮及腺上皮上持续弱表达，而PPARδ蛋白质在假孕各期子宫中均没有检测到。

MethodsEarly embryos at different stages and uterus of the non-pregnant, and pregnant uterus of 1 to 8 days pseudopregnancy in mice were obtained.

方法取小鼠早期胚胎、动情期未孕、真孕及假孕1～8d小鼠子宫，利用免疫组织化学染色及图像分析法，检测p38MAPK在不同发育阶段早胚及围植入期子宫内膜中的表达和变化规律。

There was a low level of basigin mRNA expression in the luminal epithelium on days 1 to 8 of pseudopregnancy and glandular epithelium on days 2 to 6 of pseudopregnancy.

Basigin mRNA在假孕大鼠子宫第1-8天腔上皮及第2-6天腺上皮持续表达，而basigin蛋白质则在假孕第1-2天的腔上皮及第2-6天腺上皮表达。

Methods: The fetuses of interfamily pregnancy day 4, day 8 and day 12 were acted as experimental group, while the ones of intraspcific pregnancy day 4, day 8 and day 12 of embryo transfer and the uteri of pseudopregnant mice of corresponding time were employed as positive and negative groups respectively.

以科间妊娠D4、D8、D12的孕体为实验组，小鼠同种移植妊娠D4、D8、D12的子宫孕体和相应时间假孕小鼠的子宫为阳性和阴性对照。

The healthy survivals were also transferred into the oviducts of pseudopregnant female mice. PCR was used to analyze the integration of the transgene in the genomes of mice.

选用内交系C57BL／6J种小鼠作为受精卵的供体鼠，昆明种小鼠作为假孕母鼠，将长2583bp、携带有CMV启动子和人CKLF-1基因的片段显微注射到受精卵的原核内，然后将注射后成活且健康的受精卵移植到假孕母鼠的输卵管内使其发育。

It was found that during all three stages, the expression levels of Dnmt1 mRNA in the epithelial cells of the pregnant mice were significantly lower than those in the pseudopregnant mice (P.05), and the level of Dnmt1 protein expression in the pregnant mice was significantly decreased as compared with that in pseudopregnant mice at the 4-cell stage.

结果 Dnmt1蛋白主要表达于上皮层；妊娠组小鼠各期Dnmt1 mRNA表达水平均显著性低于同期假孕组(P.05)，妊娠组4细胞期Dnmt1蛋白表达水平显著性低于同期假孕组(P.05)。

It was shown that: 1 MMP2 activity reached the highest level during proestrus, and was lowest during diestrus; 2 MM2 acti vity was increased from the first day of pregnancy, peaked on day 4 of pregnancy; but decreased significantly at day 7 of the pregnancy. 3 MMP2 activity was obviously higher on day 1 of pseudopregnancy than on the other days, and its activity decreased gradually, reached the very low level at day 4 of pseudopregnancy and maintained the low level until day 7; 4 MMP2 activity was decreased significantly 24 hours after injection of 17βestradiol ( 01 mg) at metaestrus, while MMP2 activity had no change after progesterone (04 mg) injection.

明胶酶A活性变化如下：1在大鼠的动情前期明胶酶A的活性最高，间情期最低；2在妊娠早期(1～7天)的卵巢中，明胶酶A的活性在妊娠第4天达到最高，第1～3天及第5、6天其活性也较高，但第7天则明显下降；3在假孕(1～7天)大鼠的卵巢中，明胶酶A的活性在假孕第1天最高，第2、3天略下降，从第4天到第7天，其活性都处于较低水平；4动情后期大鼠皮下注射雌二醇(0.1mg/只)24小时后，明胶酶A活性明显上升。

RESULTS:①A total of 178 injected and survived zygotes were replanted. Thirteen mice were born with the birth rate of 7%.

结果：①178只注射pcDNA3.1-mutant LMNA后的存活受精卵移植至假孕鼠输卵管后，出生13只子代鼠，出生率只有7%；58只注射pcDNA31 -mutant LMNA后的存活受精卵移植到假孕鼠输卵管后，在移植后12~15 d的受体母鼠子宫内收集到1只活胚和5只死胚。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。