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Ulex Europeus was used as a probe to study the expression of α L Fucose in mouse uterus during early pregnancy,pseudopregnancy and estrous cycle.

应用免疫荧光技术以生物素化的荆豆素-为探针,检测其结合位点α-L-岩藻糖在小鼠妊娠 1~ 8d、假孕 1~ 6 d及发情周期子宫中的表达情况,并以摘除卵巢的小鼠为模型观察雌激素、孕酮对小鼠子宫α-L-岩藻糖表达的影响。

In the uterus on day 5 of pseudopregnancy or under delayed implantation,the expression signal of CD63 was hardly to be detected,but there was a specific expression after the activation of delayed implantation.

在假孕和延迟着床的子宫中,检测不到CD63基因的表达,而在延迟激活子宫的着床位点,CD63基因的表达特异性增强,其表达模式与正常妊娠第5天着床位点处类似。

The aim of this study was to examine cPLA2α gene expression and regulation in mouse uterus during early pregnancy, pseudopregnancy, delayed implantation, artificial decidualization and hormonal treatment by in situ hybridization and immunohistochemistry.cPLA2α immunostaining was detected in the luminal epithelium and glandular epithelium of mouse uterus from days 1 to 5 of pregnancy.

本实验以小鼠为材料,利用原位杂交和免疫组化方法,检测cPLA2α基因在小鼠早期妊娠子宫中的表达,并利用假孕、延迟着床、人工蜕膜化及激素处理等模型研究cPLA2α基因在子宫中的表达与调节。

The aim of this study was to investigate PGT, 15-PGDH and CBR1 expression in themouse uterus during early pregnancy and their regulation in related models of pseudopregnancy,delayed implantation, artificial decidualization, steroid hormone treatment by in situ hybridization,RT-PCR and immunohistochemistry.

本研究利用原位杂交、免疫组织化学和RT-PCR等方法,检测了PG转运载体PGT、代谢酶15-PGDH和CBR1在小鼠早期妊娠子宫中的表达,并利用假孕、延迟着床及激活、人工蜕膜化、性腺类固醇激素处理等模型,研究了它们在小鼠子宫中的表达与调节。

The aim of this study was to investigate the expression of mPGES-2 protein in mouse oviduct during the early pregnancy, pseudopregnancy, and estrous cycle by immunohistochemistry and Western blot. The regulation of mPGES-2 protein in mouse oviducts was also studied under delayed implantation, steroid hormonal treatments and oviductal ligation.

本实验主要利用早期妊娠、假孕、输卵管结扎、发情周期、激素调节以及延迟着床等实验模型,结合免疫组织化学和Western blot 的实验方法,详细研究了mPGES-2 蛋白在小鼠输卵管中的表达与调节。mPGES-2 蛋白主要定位于不同时期的伞部和壶腹部的上皮纤毛细胞中,在伞部和壶腹部肌层以及峡部和宫管连接处的上皮和肌层中未检测到mPGES-2 蛋白的表达。

On days 1, 2, 7 and 8 of pregnancy,no signals were detected. In the pseudopregnant mice, the expression pattern of claduin-3 was alikenormal pregnancy.

在假孕模型中,封闭蛋白-3的表达情况与正常妊娠第1-5天的表达情况基本相同,在第1和2天没有表达信号,第3和4天表达量达到最高,但在第5天的子宫内膜腔上皮中有较弱表达,腺上皮中没有信号。

In situ hybridization using biotinylated POMC cRNA probe made it possible to localize POMC mRNA in the rat ovary. POMC mRNA predominantly existed in corpora lutea of diesturs, pregnant and pseudopregnant rat ovaries. POMC containing cells scattered in the ovarian intertitium were seen. The amount of POMC mRNA in granulosa cells was negligible.

采用生物素标记的POMCcRNA作为探针,进行原位杂文,研究POMCmRNA在大鼠卵巢中的分布,实验表明,POMCmRNA主要分布于间期,妊娠和假孕大鼠卵巢黄体中,间质细胞也可见少量POMCmRNA,而卵泡颗粒细胞中POMCmRNA极少。

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