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The greatest efficiency in Agrobacterium-mediated transformation of lettuce cotyledons(2~3 days-old) was obtained by 10-time dilution of Agrobacterium cells, 15-min inoculation time,1-day pre-culture,4-day co-culture and without AS using the MS(Murashige and Skoog\'s,1962) medium supplemented with 0.2 mg/L 6-benzylaminopurine,0.1 mg/Lα-naphthaleneacetic acid,100 mg/L Kan and 500 mg/L Carb,.

在农杆菌介导的皱叶生菜子叶(2~3 day苗龄)转化试验中,采用含BA 0.2mg/L,NAA 0.1 mg/L,Kan 100 mg/L,Carb 500 mg/L的MS培养基,最优的转化条件为农杆菌稀释倍数为10倍,预培养时间为1 day,共培养时间为4 day,浸染时间为15 min,不添加乙酰丁香酮。

As regards the doctrine of the Fathers, it is not possible in the present article to multiply patristic texts, which are usually characterized by wonderful beauty and clearness.

至于学说的父亲,是不可能在目前的文章以倍数计教父的内容,它们通常是由奇妙的美丽和洁净度。

The acceleration profile of the crane motion is designed to induce minimum payload pendulation during transpo rt,while the deceleration stage is designed to ensure freed om of residual oscillation near the end of the transport,and to deliver the payload at the target point.The techniques induce payload pendulation at least one half ofan oscillation cycle or integer multiples during each of the acceleration and deceleration stages.Starr(1995)designed a symmetric two-step acceleration and deceleration profile to transfer a suspended object.The duration on the constant acceleration steps is assumed to be negligible relative to the period of the payload oscillation.A linear approximation method of the oscillation period is used to generate the two-step symmetric acceleration profile.

3.1投入成型技术,各种形式的投入shapers , convolved参考命令,产生形投入指挥,驾驶起重机提出一套距离沿一套path.the加速度概况起重机议案旨在诱使最低载重pendulation期间transpo rt ,虽然减速阶段,目的是确保freed om残余振荡接近尾声的运输,并交付载荷到达目标point.the技巧诱使载荷pendulation至少一半ofan振荡周期或整数倍数

The protein was detected as multiple small perinuclear foci and localized in the virus assembly sites.

该蛋白先散在于细胞核周围,然后向病毒养生装配部位集中(放大倍数100×)。

The allomatric scaling for the wet clonal plants including Phalaris arundinacea Scirpus planiculmis Phragmites australis and Phragmites Jeholensis did not show "1/4-power Rule". The variation of the allometric exponents between vegetative and reproductive ramets was mainly due to the different regulation mechanisms in different development stages. Moreover, the variation of the allometric exponents between plantsin wet or drought environments was the result of the long-time divergent response for ramets to moisture environments.

虉草、紧穗三棱草、芦苇和热河芦苇4种湿地根茎植物分株异速生长规律并不表现为&1/4或1/4整倍数&规律,营养株和生殖株异速生长幂值的差异是不同发育类型的分株生长调节机制不同的结果,水生生境和旱生生境分株异速生长幂值的差异则是分株对生境水因子差异长期趋异响应的结果。

The nickel, lead, cadmium and mercury ions were pre-column derived by tera-(2-aminophenyl)-porphine (T2APP). These chelates were enriched by solid phase extraction with C18 cartridge and the enrichment factor of 50 was achieved.

中草药样品用微波消化,样品消化液中的镍、铅、镉、汞用四--卟啉(T2APP)柱前衍生,然后用ZORBAX RP18固相萃取小柱萃取富集镍、铅、镉、汞的T2-APP络合物,富集倍数为50倍;经富集后的络合物用乙腈和0.05mol?

This paper investigated the physical properties of polyester filament yarn such as tenacity,elongation and boiled water shrinkage by means of analyzing the POY which had been treated by different draw ratio and heating temperature.

通过对不同拉伸倍数及不同温度处理的POY的分析,探讨长丝纱的强度、伸长度及沸水收缩率等物理机械性质。

A preconcentration factor of 250 was achieved in this method.

用这种方法达到了250的富集倍数

Methods By means of UPLC, apply factorial experiment design to investigate overall the changing rule of the contents of salvianolic acid B,tanshinol,protocatechualdehyde and etc.in salvia miltiorrhiza with different multiple of water、decocting duration,decocting times.

方法使用超高效液相色谱仪,采用析因试验设计,考察了丹参饮片在不同加水倍数、煎煮时间、煎煮次数下的煎出液中丹酚酸B、丹参素、原儿茶醛等多种成分的含量变化规律。

The 1/2 MS medium supplemented with 1.0 mg·L-1 BA and 0.1 mg·L-1 NAA was very beneficial to the protocorm differentiation and propagation of D.candidum .The highest protocorm propagation index was obtained from the medium containing the activated carbon.The highest root numbers and length were observed in plants growing in 1/2 MS medium containing 0.5 mg·L-1 NAA.

结果与结论:添加20%马铃薯液有利于种胚的萌发,种胚萌发率为79.37%;BA 1.0 mg.L-1和NAA 0.1 mg.L-1最有利于原球茎增殖,培养基中添加2%活性碳原球茎增殖倍数高;NAA有助于试管苗的生根,不同含量NAA以0.5 mg.L-1时试管苗平均根数最多和平均根长最长。

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It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。