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In the self-bred progenies of autopolyploid,we found that a very few chimaeraexist in the cells of root tips,but most of them had no change in ploidy. We also found13 diploid plants in the experimental plot (ratio is 0.46%) and can be divided into 4categories.

在部分同源多倍体株系的自交结实后代中,随机取样进行根尖染色体检查,结果表明多倍株系体的自交结实后代倍性没有变化,但发现有个别细胞为不同倍性的嵌合体;田间观察到分离出来的极少数正常二倍体,占0.46%,按照形态特征可以分成四个不同的类型。

The results indicated that chromosome counting was the most reliable and precise method, however, it was cumbersome and required skilled cytological techniques; The chromocenter size, heterochromatin number, chroloplast number of guard cell and certain plant morphological characteristics are simple and practical methods for ploidydetermination but with some shortages.

结果表明:对体细胞染色体的直接计数法是最可靠的方法,但费时、难度大;叶片气孔大小与倍性无显著相关性;保卫细胞叶绿体数目、体细胞染色中心大小以及异染色质个数与黄瓜倍性密切相关,是简单、快速而有效的倍性鉴定方法;植株的形态学观察也能间接确定植株倍性,但鉴定时期偏晚,精确性较差。

F〓 of transgenic allotetraploid fish containing"all-fish"transgene——pCAgcGHc was obtained respectively by inbreeding and gynogenesis. Positive rate of pCAgcGHc detected by PCR was 90% in F〓 of transgenic allotetraploid fish produced by inbreeding, while most F〓 of transgenic allotetraploid fish produced by gynogenesis showed diploidy measured by flow cytometer. The results above imply that developing the pure line of transgenic allotetraploid fish through inbreeding is feasible, but the operation should be modified if gynogenesis is introduced.

采用近交和雌核发育方法培育出转pCAgcGHc基因异源四倍体鲫鲤F〓代,通过PCR检测,外源基因pCAgcGHc在近交转pCAgcGHc基因异源四倍体鲫鲤F〓中的整合率为90%,而流式细胞技术检测雌核发育转pCAgcGHc基因异源四倍体鲫鲤F〓的倍性多为二倍体,说明通过近交筛选转基因异源四倍体鲫鲤纯系的方法是可行的,但是通过雌核发育来筛选转基因异源四倍体鲫鲤纯系还要就方法上进一步的改进。

Since curly leaf hybrid could be recognized at early stage,and displayedobvious dwarfness,choosing"Zha′ai 76"as paternal parent not only raised thehybrid-selecting efficiency from the pollinated progenies,but also raised thebreeding efficiency of apomctc dwarf roostock of apple.

鉴别宽叶型杂种较难、需时较长(2-3年生时)并需与染色体倍性鉴定法相结合,因其形态变化较小而生长势又与平邑甜茶无融合生殖苗无明显差异;鉴别光叶型杂种因父本染色体组的影响加大而从形态上容易些,但也需过氧化物同工酶法或染色体倍性鉴定法相配合;皱叶型杂种因在苗期刚出1-2片真叶时就表现明显的标志性状、能从叶片形态上准确鉴别出来,毋须染色体倍性鉴定等复杂方法;因皱叶型杂种能早期鉴别,又表现明显的矮生性,因而选用"扎矮76"做父本不仅大大提高了平邑甜茶授粉后代中选择杂种的效率,而且提高了苹果无融合生殖型实生矮砧育种的效率。

Euploidy The normal state in which an organism ' s chromosome number is an exact multiple of the haploid number characteristic of the species.

倍性:正常状态下,生物体染色体数是此种生物单倍体数目整数倍的现象被称为整倍性

The number of chloroplast in stomata guard cell, density, length and width of stomata were observed.

以苹果和梨的二倍体和多倍体为试材,比较了气孔保卫细胞叶绿体数目、气孔密度、气孔长和气孔宽四个性状与倍性的关系,分析各个性状用于倍性鉴定的可靠性,并采用两类性状同时进行判别分析,建立判别分析方程。

The results showed that the number of chromosome, which was from the cultured seedlings of meristem tip and the rank seedlings of meristem tip, was not altered, both the number were 2n=16,the hereditary stability of callus and regeneration plant was poor; and the variation percentage of chromosome in callus was 43.4%, among which haploid accounted for 6.7%, triploid accounted for 2.5%,tetraploid accounted for 10%,pentaploid accounted for 4.2%,hexaploid accounted for 3.3%,septuploid accounted for 4.2%,octoploid accounted for 3.3%,dysploid accounted for 9.2%;the chromosome variation percentage of differentiation seedlings derived from callus was11.7%,among which haploid was 6.7%,triploid accounted for 1.7%,tetraploid was 3.3%.

结果表明,茎尖分生组织培养的幼苗及丛生苗遗传稳定,其染色体未发生倍性变异,均为2n=16;愈伤组织及其再生苗遗传稳定性较差,愈伤组织染色体数变异率为43.4%,其中单倍体占6.7%、三倍体占2.5%、四倍体占10%、五倍体占4.2%、六倍体占3.3%、七倍体占4.2%、八倍体占3.3%、非整倍体占9.2%;愈伤组织分化苗染色体变异率为11.7%,其中单倍体占6.7%,三倍体占1.7%,四倍体占3.3%。

The phenomenon of polyploidy and aneuploidy exists in the Genus, in which there are polyploid complex.

对19个分类群作了核型观察,其中16个分类群作了组型分析,结果表明各分类群间核型差异显著,该属普遍存在着多倍性和非整倍性现象,并有多倍体复合体类群存在。

Using nuclear DNA C-values for 539 angiosperms in China, we examined the variation of these values among growth forms and taxonomic groups and the relationship of these values with invasiveness. Mean DNA C-value of the 539 angiosperm species was 4.06 pg. Mean DNA C-value was(1) significantly lower for woody species (1.84 pg) than for herbaceous species(5.02 pg);(2) significantly lower for 360 dicots (2.20 pg) than for 179 monocots (7.80 pg);(3) significantly lower for annuals (2.78 pg) than for perennials(6.65 pg);(4) significantly lower for 134 weed species (1.93 pg) than for herbaceous non-weeds (6.75 pg) and for several families that have an unusually high proportion of weed species;(5) significantly lower for 47 exotic weed species (1.76 pg) than for 134 native weeds (1.93 pg), but significantly lower than that of "non-weedy" herbaceous species (6.75 pg);(6) lower for weeds than for "non-weedy" species in same genus or family; and (7) in herbaceous species, generally lower for weedy compared to "non-weedy" species, with some exceptions such as Avena fatua, whose DNA C-value is as high as 14.15 pg, contrarily, and some "non-weedy" herbaceous species in Cruciferae and Cucurbitaceae with very low values.

统计了中国境内有分布的539种被子植物的DNA C-值,分析了它们在不同分类群、生活型、倍性、生活史类型以及在杂草和非杂草类群中的分布情况,主要结果如下:(1)539种被子植物DNA C-值平均为4.06 pg,其中木本植物的DNA C-值平均为1.84 pg,低于草本植物的平均值(5.02 pg);(2)双子叶植物(360种)的DNA C-值平均为2.20 pg,极明显地小于单子叶植物(179种)的平均值(7.80 pg);(3)1年生植物的DNA C-值平均为2.78 pg,明显小于多年植物的平均DNA C-值(6.65 pg);(4)134种杂草的DNA C-值平均为1.93 pg,明显小于非杂草草本植物的平均值(6.75 pg),含杂草较多的科,平均DNA C-值相对较小;(5)统计的47种入侵杂草的DNA C-值平均为1.76 pg,略小于134种杂草的平均DNA C-值(1.93 pg),极显著地小于非杂草性草本植物(6.75 pg);(6)以科为单位,不同科的DNA C-值存在着极大的差异;(7)DNA C-值与染色体倍性的关系并不明显,但是,随着倍性的增加,基因组变小;(8)在同一科、属中,与非杂草相比,典型杂草的DNA C-值往往偏小;(9)总体上杂草或杂草性强的植物,它们的DNA C-值比非杂草性植物的要小。

Most primers amplified same bands in diploids, tetraploids and hexaploids. It suggest that D and AB genomes have high homology and D genomes be successfully transferred from diploid to hexaploid.

从供试的绝大多数引物均能在不同倍性小麦中扩增出相同的条带证明了D染色体组和AB染色体组间存在着很高的同源性以及利用二倍体和四倍体小麦杂交人工合成六倍体小麦的真实性。

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