修饰

This study aims at the application of ESI-MS and MALDI-MS in the identification of acetylation of mouse liver.

本论文的研究工作旨在考察两类生物质谱技术ESI-MS和MALDI-MS在乙酰化蛋白质翻译后修饰鉴定中的应用及其应用于小鼠肝乙酰化修饰谱的鉴定研究。

The methylated amidogen still held protonation at low pH. Therefore, the adsorption ability of biomass was almost unchangeable through methylation of amidogen in biomass. The acetylated amidogen lost protonation at low pH, and the capacity of dye adsorption of biomass decreased by 51.6% after acetylation of amidogen in biomass.

甲基化氨基在酸性条件下仍然可以质子化，故氨基甲基化修饰后染料吸附性能不变；乙酰化氨基在酸性体系中失去质子化能力，乙酰化修饰菌体染料吸附性能下降51.6%。

In this research, B2 gene of HCV was highly expressed as a fusion protein (Trx-B2) by cloning into pThioHisA, and induced specific anti-HCV antibody in higher titer than unmodified B2 protein after immunize mice or rabbit.The results showed antigen Trx-B2 has obvious immunogenicity and induced specific antibody,which might be able to serve as diagnosis HCV. Consequently, this PcAb of rabbit was used to coated Immulon plate, and a method of both antibodys sandwiching antigens was developed to detect 32 HCV positive serum and 32 nomal serum.Results show that ratio of positive are 87.5%and 43.75% respectively.

但缺点是该抗原所诱导的抗体滴度较低，可能是由于其分子量较小，所以本课题将人工合成的HCV复合多表位抗原基因B_2克隆到表达载体pThioHisA中，利用硫氧还蛋白融合方式对该抗原进行修饰，并在大肠杆菌中表达融合蛋白(Trx-B_2)，纯化该蛋白后免疫小鼠及家兔，结果表明用硫氧还蛋白修饰后的融合蛋白免疫小鼠和家兔后，得到的抗体滴度均高于未经修饰过的复合多表位抗原合成肽所诱导的抗体滴度，并从免疫后的兔血清中提取IgG包被酶联板，利用双抗夹心法分别检测了32份HCV阳性血清和32份正常人的血清，结果阳性率分别为87.5%和43.75%，符合率为71.88%。

In contrast, mass spectrometric measurement containing ESI-MS and MALDI-MS not only allows posttranslational modification characterization but also reveals the number of acetyl groups attached to the protein.

软电离生物质谱技术ESI-MS和MALDI-MS能很好地应用于翻译后修饰的鉴定研究，不仅能检测到翻译后修饰蛋白，且能得到具体的修饰位点信息。

First of all, a systematic study on adsorption properties of the four phenolic compounds (phenol, p-chlorophenol, p-cresol and p-nitrophenol) on MX-4, AM-1 and NJ-8 was carried out, and a comparison of the adsorption behavior between the new resins and Amberlite XAD-4 was also made. In addition, the impact of the resin structure and the property of a specific compound on adsorption behavior was discussed herein.

用静态吸附法系统地研究了四种酚（苯酚、对氯苯酚、对甲苯酚、对硝基苯酚）在乙酰基修饰交联聚苯乙烯树脂(MX-4)、酚羟基修饰超高交联聚苯乙烯树脂(AM-1)以及氧修饰超高交联聚苯乙烯树脂(NJ-8)上的吸附行为并与AmberliteXAD-4树脂的吸附行为进行比较，探讨了树脂结构和化合物性质对吸附行为的影响。

Analysis of the relation between serotonin and its receptor in OFC and stress depression(n=8):(1) Microinjection of 5-HT into OFC significantly shortened immobility time, dejections have no great changes; though locomotor、 grooming activitives have little increased, they have no significant difference; rearing scores greatly reduced;(2)microinjection of pCPA into OFC significantly prolong immobility time, dejections have no great changes; locomotor、rearing activities are greatly reduced, but have no significant effect on grooming activity receding;(3)compared with the effect of 5-HT alone, microinjection of spiperone combined with 5-HT into OFC, significantly prolong immobility time and greatly reduced dejections; locomotor、 rearing、 grooming activities are all reduced, but have no significant differences.

眶额叶区5-羟色胺(5-hydroxytryptamine，5-HT)及其受体与应激性抑郁症的关系(n=8)：(1)注射5-HT后，大鼠FST不动时间明显降低(P＜0.05)，粪便数量变化不显著(p＞0.05)，OFT水平运动、修饰得分虽均有升高，但无显著性差异(P＞0.05)，垂直活动显著降低(P＜0.05)；(2)注射色氨酸羟化酶抑制剂-对氯苯丙氨酸后，FST不动时间明显增加(P＜0.01)，粪便数量无显著性变化(p＞0.05)，OFT水平、垂直运动显著减少(P＜0.05)，修饰降低但无显著差异(P＞0.05)；(3)与5-HT组比较，先注射5-HT_(1A)受体拮抗剂后再注射5-HT，FST中的不动时间明显增加(P＜0.01)，粪便数量显著减少(p＜0.01)，但OFT水平、垂直、修饰活动均有所减少，却无显著性差异(P＞0.05)。

Modification right of biogenetic resource should be practiced according to the principles of "preservation","good-will" and "preventing ill-doing".

生物遗传资源的修饰权由人类本着&保存&、&善意修饰&、&预防恶意修饰&的原则掌握。

It has been proved that surface confined quinones can greatly impove the electrocatalytic activity for ORR.

本论文中研究的氨基蒽醌聚合物修饰电极，与其它方法得到的醌类修饰电极比较，具有性质稳定、制备过程简单、活性点位浓度高等优点，而且同时具有类似聚苯胺的长链结构和蒽醌官能团的特性，为制备具有高氧还原催化活性的蒽醌修饰电极的研究提供了新的思路。

Poly-aminoanthraquinone modified electrode is more stable, more easily prepared, and can provide higher surface concentration of reactive sites compared with other quinones modified electrode by adsorpsion or doping.

本论文中研究的氨基蒽醌聚合物修饰电极，与其它方法得到的醌类修饰电极比较，具有性质稳定、制备过程简单、活性点位浓度高等优点，而且同时具有类似聚苯胺的长链结构和蒽醌官能团的特性，为制备具有高氧还原催化活性的蒽醌修饰电极的研究提供了新的思路。

A word or word group, such as an adjective, that is placed adjacent to the noun it modifies without a linking verb; for example, pale in the pale girl.

修饰语，修饰性形容词一个词或词组，例如一个形容词，不用连接动词，紧接于它所修饰的名词之后；如在脸色苍白的小女孩里，苍白的

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。