信息学

Bioinformatics studies of the functional impact of alternative splicing have revealed a wide range of regulatory mechanisms, from NAGNAG sites that add a single amino acid; to short peptide segments that can play surprisingly complex roles in switching protein conformation and function (as in the Piccolo C2A domain); to events that entirely remove a specific protein interaction domain or membrane anchoring domain.

选择性剪切的功能性影响的生物信息学研究揭示了各式各样的调控机制，从添加一个单氨基酸的NAGNAG位点；到可以在开关蛋白质构象和功能(如在Piccolo C2A区域)中起到惊人地复杂作用的短肽片段；到完全移除一个特定蛋白质相互作用区域或膜锚定区域的事件。

Bioinformatics analysis showed that the gene sequence was highly consistent with previously reported F3,5H genes, such as GenBank Accession D14588, Z22545, and DQ352142. The encoded amino acid sequence was highly homology with that of GenBank Accession BAA03438.1, BAC10997.1 (Nierembergia sp. NB17) and AV85470.1 at 99%, 88% and 87%, respectively.

生物信息学分析表明克隆的F3，5H基因与GenBank登记号D14588、Z22545和DQ352142等的F3，5H基因序列高度一致；其编码的氨基酸序列与GenBank登记号BAA03438.1、BAC10997.1和AAV85470.1的高度同源，同源率分别为99%、88%和87%。

The bioinformatics analysis of the deduced amino acid sequences of four genes indicated they are classical AGPs. They contain an N-terminal signal sequence, a central domain rich in Ala, Ser, Thr, Pro/Hyp, and C-terminal hydrophobic transmembrane domain. All of them include two AGP-like domains(rich in noncontinuous Pro residues) and a putative C-terminal GPI anchor signal sequence.

对四个基因编码的蛋白序列进行生物信息学分析，发现它们都包含N端信号肽序列，具有富含Ala、Ser、Thr、Pro／Hyp的长度可变区以及C端疏水跨膜区，还具有两个富含非连续Pro的AGP-like domain，C端存在可能的GPI锚定区。

This ORF included 1101bp, encoding 367 amino acids, 92% similar to human and mouse.

结果分离的ORF全长1101bp，编码367个氨基酸，与人鼠氨基酸序列92%同源；利用生物信息学软件分析出此蛋白的理化特性并预测了其一级、二级和高级结构。

We report here the cloning and characterization of a novel human PanK gene.

从我们的大规模cDNA克隆和测序计划中，利用表达谱基因芯片筛选的结果并通过生物信息学分析，我们选取了4条基因进行进一步的研究，以探讨这些基因是否与疾病相关。

Optimization and parallelization of others classical bioinformatics problems.

其他有代表性的生物信息学算法进行了优化和并行化。

Bioinformatic analysis of this gene revealed that this protein belonged to the member of family 35 of glycoside hydrolyase superfamily 42, and had a closer genetic relationship with Arabidopsis thaliana, but farther with Persea americana and Picea sitchensis.

生物信息学分析结果表明，该番木瓜β-GAL属于糖苷水解酶超级家族42中家族35的成员，在进化过程中与拟南芥的亲缘关系较近，与鳄梨和北美云杉则较远。

A large prolyl peptide bond dataset was constituted from the list of the structures which had resolution better than 0.25nm, sequence identity between each pair of the sequences less than 30%.

因此，本论文从生物信息学的角度，对蛋白质中的脯氨酸进行了较为系统的研究。

In this study, the genomic structure and regulatory elements of ApoD from 10 representive species including protozoan, invertebrate, protochordate and vertebrate were analyzed and compared. The genomic structure of ApoD is less conserved in organisms from protostome to deuterostome invertebrates, while it is highly conserved among chordates including amphioxus and verebrates. All four conserved cysteine residues are present in amino acid sequence of deuterostome ApoDs, while there are only two cysteine residues in amino acid sequence of protostomes ApoD. Structure divergence between protostome and deuterostome ApoD proteins suggests their function difference. The majority of regulatory elements are present in nearly all organism ApoD genes ranging from unicellular protozoan to mammals, suggesting that ApoD plays a very fundamental role, and possesses a conserved regulatory mechanism. However, there also exist some specific regulatory elements, which are present only in certain species and may perform some special roles.ApoD mRNA expression in murine NIH/3T3 fibroblasts exposed to various stresses such as as hydrogen peroxide and UV light shows dose-dependent increase. And a fly homolog of ApoD, Glial Lazarillo, whose overexpression results in increased resistance to hyperoxia as well as a extension of lifespan under normoxia and resistance to starvation without altering lipid or protein content.

本文首先从生物信息学角度对分属于原生动物、无脊椎动物、头索动物和脊椎动物类群的10种动物ApoD的基因结构及调控区的调控元件进行分析及比较，发现：(1)ApoD基因外显子-内含子结构从原生动物草履虫到原口动物再到后口动物海胆的进化过程中不保守，但在分析的几种脊椎动物中相当保守；(2)文昌鱼ApoD基因扮演从无脊椎动物到脊椎动物承上启下的角色，可能代表了脊椎动物ApoD基因原型；(3)四个半胱氨酸保守位点在后口动物中都存在，而在原口动物中只存在两个，原口、后口动物ApoD蛋白一级结构上的差异反映蛋白功能上可能也存在一定差别；(4)调控区大多数主要调控元件为不同动物共有，说明ApoD主要功能及其表达调控在进化中相当保守；(5)ApoD基因个别调控元件是随着物种进化而出现并开始发挥相关作用，如SF-1；还有一些调控元件在进化过程中还没有发现其规律，这说明ApoD某些功能和基因表达调控模式可能因物种不同而存在一定的差异。

Firefly luciferase activities were measured by double luciferase assays System,and Renilla luciferase was used as internal control.

生物学信息学预测VEGF可能是miR-126的靶基因，miR-126通过作用于VEGF的3\'UTR端而起到抑制基因表达的作用。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。