信号物

Methods Weak optical signals were acquired with hypothermal cooled CCD camera. The system captured the fluorescent images of different projections at rotating the tissue phantom.

系统采用低温制冷CCD相机作为高灵敏弱光信号探测器，通过旋转目标物，得到各个角度的激发荧光场。

Gondii, were cloned by PCR respectively. The PCR products were digested by the corresponding enzymes and ligatd into the intermedial vectors. Finally, the inducible RNAi vector, pBSK-HSP70/5UTR-IntronC-HSP70/3UTR, with the HSP70 gene promotor as a promotor, the intron C sequence ofβ-tubulin gene as intervening sequence, 3UTR sequences of HSP70 gene as transcription stop signals, was constructed successfully, and the results of sequencing were correct. 3The construction of the inherited and inducible RNAi vector system of T. gondii: The fragment of SAG1/5UTR-eGFP-SAG1/3UTR in pBSK-SAG1/GFP vector was cloned into the vector of pBSK-HSP70/5UTR-IntronC-HSP70/3UTR to construct pBSK-GFP-Hairpin vector, then the fragment of GFP-Hairpin in pBSK-GFP-Hairpin vector was cloned into pHANA-0.5 vector.

弓形虫可诱导的反向重复序列RNAi载体的构建：设计引物，通过PCR分别扩增弓形虫HSP70基因5&UTR启动子序列(HSP70／5UTR)、HSP70基因3&UTR序列(HSP70／3UTR)及β-微管蛋白基因内含子C序列，通过酶切连接，构建以弓形虫热休克蛋白HSP70基因启动子进行驱动的，以β-微管蛋白基因内含子C序列作为间隔序列，以HSP70基因3UTR序列作为转录终止信号的反向重复序列RNAi载体pBSK-HSP70／5UTR-IntronC-HSP70／3UTR，序列测定结果正确。

However, there is now increasing evidence that CD44 is assembled in a regulated manner into membrane-cytoskeletal junctional complexes and, through both direct and indirect interactions, serves to focus downstream signal transduction events.

然而，现在越来越多的证据表明CD44的装配以一种调控模式嵌入膜骨架复合物，并通过直接和间接的相互作用为下游信号转导重点服务。

Methods The α-factor prepro peptide from Saccharomyces cerevisiae was used as a signal peptide to secret HBsAg in Hansenula polymorpha. Both MFα and HBsAg genes were designed based on Hansenula polymorpha-preferred condons and synthesized by overlap PCR respectively, then fused together by PCR using a pair of primers.

在HBsAg基因前加酿酒酵母α前导肽基因序列作为信号肽，将整个序列优化为汉逊酵母优选密码子，采用搭桥PCR方法分别合成后，通过1对引物PCR法融合在一起。

The binary complex of Al-DNAs then interacts with tetraphenylporphyrin in tetrachloromethane, forming a ternary complex of TPP-Al-DNAs.

该复合物469nm产生强烈增强的BRLS信号，并且I_正比于ctDNA和fsDNA的浓度，可用于灵敏的测定核酸，方法的检出限在皮克级。

Then the undissolving deposition was formed on the electrode surface by using HRP catalyze hydrogen peroxide to oxidize 3, 3'-diaminobenxidine tetrahydrochloride dihydrate. So the electrochemical signal of the electrode is enlarged greatly.

通过HRP催化双氧水氧化3，3'-二氨基联苯胺在电极表面形成不溶性沉积物，从而放大电化学检测信号。

Chicken IL-15(ChIL-15), Which was discovered in 2001, plays a main role in activating NK cells and CD8+ memory T cells, and may therefore have potential as a vaccine adjuvant.According to published ChIL-15 gene sequence, a pair of primers were designed and synthesized to clone ChIL-15 cDNA from ConA-activated chicken splenocytes by RT-PCR. Recombinant plasmid pUC19ChIL-15 carrying the ChIL-15 gene was constructed. The gene encoding mature ChIL-15 (mChIL-15) was amplified from pUC19ChIL-15 by PCR and cloned into pMD 18-T vector. After digested with Kpn I /Pst I , the mChIL-15 gene was subcloned into prokaryotic expression vector pPRoEX橦Ta and transformed into E.coli DH5a .The transformant was induced by IPTG, and the recombinant ChIL-15(rChIL-15) was expressed as a fusion protein with a histidine hexamer tag at the N-terminal end of the protein. Following expression, the protein was purified by ProBond?Nickel-Chelating Resin. The uninduced and induced protein lysates and the purified protein were separated by SDS-PAGE and transferred onto nitrocellulose membrane to identify rChIL-15 by Western blot. The rChIL-15 was used to immune the guinea pig to obtain rChIL-15 polyclonal antibody.

参考已发表的ChIL-15基因序列，设计合成引物，应用RT-PCR技术从刀豆球蛋白A活化的白来航鸡脾淋巴细胞中克隆ChIL-15 cDNA，将其与SmalⅠ酶切处理的pUC19载体连接，构建重组质粒pUC19ChIL-15；用PCR技术从pUC19ChIL-15中扩增出去信号肽的成熟ChIL-15(mChIL-15)基因，与pMD 18-T载体相连构建重组质粒pMDChIL-15；然后用KpnⅠ／PstⅠ双酶切下mChIL-15基因片段，定向亚克隆到经同样酶切处理的带有6个组氨酸标签的表达载体pP_RoEX~HTa中，构建重组质粒pP_RoChIL-15，对其测序确认读框正确后，转入大肠杆菌DH5α中诱导表达并进行纯化，用重组ChIL-15(rChIL-15)免疫豚鼠，制备多克隆抗体；再用HindⅢ／PstⅠ从质粒pP_RoChIL-15上切下mChIL-15基因，定向亚克隆到经同样酶切处理的杆状病毒转移载体pMelBacB中，经酶切、PCR和序列测定鉴定后，与杆状病毒线形化DNABac-N-Blue~(TM DNA共转染Sf9昆虫细胞，经三轮蚀斑纯化，构建重组杆状病毒rBac-ChIL-15，用该重组病毒感染处于对数生长期的Sf9细胞，按不同的时间收取样品，离心后对其上清和沉淀进行SDS-PAGE和Western blot分析。

BaP can block signaltransductions、cell growth and differentiation, form adducts with DNA, induce DNAbreaks, as well as cause mutation in p53 gene.

BaP可阻断细胞生长分化调节的信号转导，与DNA形成加合物，导致DNA的断裂，诱导p53基因突变等等。

A strong and stable RTP signal can be observed due to the formation of β-CD/CBL/DBP inclosing complex and the influence of the heavy-atom perturbation.

CBL、β-CD和DBP形成三元包络物和DBP的微扰作用，CBL有强而稳定的RTP信号。

By instrumenting structures with a vibration sensor system, structural health monitoring aims to provide reliable and economical approaches to monitor the performance of structural systems in the early stage so as to facilitate the decisions on structure maintenance, repair and rehabilitation.

对结构损伤进行识别可以看作是一个模式识别课题，即抽取结构物的特征信号，然后对其进行识别分类。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。