保护剂

After confluence, there is no clear margin between cells and only nuclear apophysis could be observed. Cell monolayer was smooth like quiet lake surface.

自制的胚胎冷冻液含有三种不同的冷冻保护剂(包括渗透性和非渗透性保护剂)，冷冻胚胎解冻后可用率在95％左右，比常用的DMSO冷冻液更适宜于这三种细胞的冷冻保存。

Copper and copper prevent discoloration protective agent, copper prevent oxidant, galvanization passivation blocker, silver protectant, LED silver protectant, prevent discoloration, gold, silver, and gold, protective agent, we hole of tin additive is not completely, and formaldehyde environmental protection coating resistant color effect very good, long time in the air will become yellow, oxidation.

铜保护剂、铜防变色剂、铜防氧化剂、镀锌钝化封闭剂、银保护剂、LED银保护剂、银防变色剂、金保护剂、金封孔剂，我们的各种锡添加剂完全是不含甲醛环保型，并且镀层抗变色效果很好，长时间在空气中不会变黄、氧化。

PartⅡFeasibility of vitrifying normospermic samples using droplets without cryoprotectans Objective Discuss the feasibility of droplets to vitrify normospermic samples without cryoprotectants. Methods Normal prepared sperm were frozen by conventional freezing protocol and non-cryoprotectant freezing in droplets respectively. Freezing in droplets means freezing sperm in every 10ul drop. After thawing, compared the two groups'sperm mobilities.

第二部分微滴法无保护剂玻璃化冷冻正常精子的可行性探讨目的探讨在不添加冷冻保护剂的情况下微滴法玻璃化冷冻正常精子的可行性方法收集16份正常精液标本，上游处理后分别进行慢速冷冻和微滴法冷冻，微滴法即用拉细巴氏管将标本形成10ul的液滴，直接投入液氮内冻存，复苏后镜检其活动率。

Trehalose、adonitol、sucrose、lactose、maltodextrin、Na glutamate、glycerol、sorbitol were good cryoprotectants. The efects of cryoprotectants in different suspension medium was obvious various skim milk was a excellent cryoprotectants.

海藻糖、脱脂乳、侧金盏花醇、蔗糖、乳糖、麦芽糊精、谷氨酸钠、山梨醇等具有较好的冷冻保护效果，不同的悬浮介质内各冷冻保护剂的作用效果有很大差异，在水或培养液中保护作用表现明显，脱脂乳仍是一种良好的冷冻保护剂。

The diluent contain magnesium chloride, potassium citrate, sodium glutamate, dipotassium hydrogen phosphate, monopotassium phosphate and fructose, semen vitality is hightest after frozen.The average value is 0.52,highter than other 5 diluent significantly (P﹤0.01).So chose this diluent as semen cryopreservation diluent on chicken. Definite cryoprotectant, equilibrium time and thaw temperature:On the base of test one,research freeze sheltering effect about glycerin, DMSO ,DMA and DMF, each with 4 consistency.The result showed that 10%DMSO sheltering effect is the best, the next is 4%DMA,semen vitality are 0.58 and 0.55 after frozen.

冷冻保护剂、平衡时间和解冻温度的确定在试验一的基础上，研究甘油、二甲基亚砜、二甲基乙酰胺、二甲基甲酰胺4种冷冻保护剂在4种浓度下对鸡精子的冷冻保护作用，结果表明，10%DMSO保护效果最好，其次为4%浓度的DMA，精液冻后活力分别为0.58和0.55，两者之间差异不显著(P﹥0.05)。

The mixture of 50% non-fat milk power and 50% starch is the more effective and cheaper carrier. The concentration of Lactobacillus fermentum and Lactobacillus plantrum is 1. 21 X 1010 cfu/g , 9. 84 X 109 cfu/g in powder starter with glycerol added and the cell survival is still 65.2% and 69. 1%.respectively, after 90 days storage.

对用于制备干粉发酵剂的载体和保护剂进行了筛选，结果显示用含脱脂奶粉和淀粉各半的混合物作载体、用甘油作保护剂制备乳酸菌干粉发酵剂效果较好，发酵乳杆菌和植物乳杆菌的活细胞数分别可以达到1.21×10~(10)cfu·g~(-1)、9.84×10~9cfu·g~(-1)，保藏90天后，细胞存活率为65.2％、69.1％。

MethodsOur study was to treat gastric ulcer by invigorating spleen and benefiting vital energy Chinese drugs through clinical observation and experimental research, and compared with mucosa protectant -gastropine and H2 receptor antagonist -famotidine about the mucosa mitochondrion 8-oxygen guanine DNA glycosylase, thymine glycol DNA glycosylase,3-methyl adenine DNA glycosylase, the effect of gastralgia, splenic asthenia and quality of ulcer recovery.

比较健中愈疡片、黏膜保护剂胃舒平以及H2受体拮抗剂法莫替丁对胃黏膜线粒体 8－氧鸟嘌呤DNA糖基化酶、胸腺嘧啶乙二醇DNA糖基化酶、3－甲基腺嘌呤DNA糖基化酶的影响，并进行健脾益气方药与黏膜保护剂胃舒平以及H2受体拮抗剂法莫替丁对胃溃疡愈合质量的比较和评价。

C, 200rpm for 5d then placed statically for more than 7d, and addition more than 0.01% Chitosan to Bacillus subtilis suspension provided significant UV protection. The survival spore rate is 90% about 2-2.5 fold better than control for spore exposed to UV radiation for 30 min. Bioassay in against Glomerella cingulata, and the inhibition activity is still 60%.

枯草杆菌於MIY液态培养基中，30°C，200rpm震荡培养五天后静置培养七天以上，以甲壳素添加浓度大於0.01％作为最适宜的紫外保护剂之作用最好，於UVB灯下暴晒30 min，对枯草杆菌孢子的保护效果最好，存活率达90％以上，抗紫外线活性是不添加紫外线保护剂的CK组的2.5-3倍，对檬果炭疽真菌仍有60%的抑菌活性。

Results At -196℃,while cryoprotectors was 15% dimethyl sulphoxide and 6% propanediol,the viability of adipose tissue was the best,which reached to 76%.

低温保护剂能明显保持冷冻脂肪组织活力，不同冻存条件下其最适宜的低温保护剂各异。

All kinds of protectants were studied under the freeze-dry condition and four types of protectants, which have good effect, were screened out.

真空冷冻干燥过程中使用的各种保护剂作了详细的研究，在各种保护剂中筛选出4种效果比较好的类型，配合成复合保护剂，在很大程度上提高了双岐杆菌在冻干过程中的活菌存活率。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。