保守地

This fortress was his post as king's attorney, all the advantages of which he exploited with marvellous skill, and which he would not have resigned but to be made deputy, and thus to replace neutrality by opposition.

他极其巧妙地运用了这个职位所带来的种种优势，不管在什么情况下，他都决不辞职，至多只请人暂时代理一下，以此避免反对的立刻而始终处于保守中立。

Confined with the right to sex trade and the right to sex work, the policy of the sex trade should be governed by light-handed regulation, regardless of the prostitutes, their clients or the pimps of prostitution.

因此在性交易自由与性工作权的制约之下，国家对於性交易之管制应采取极为谦抑的保守态度，从而无论针对娼妓行为、嫖客行为或助娼行为，均不应理所当然地以处罚为首选之手段。

These mutations greatly changed the level of protein synthesis, lipogenesis and JAK2 phosphorylation stimulated by GH, although they did not affect the expression of the GHR and ligand binding.

研究结果表明豚鼠GHR中的一些氨基酸残基的取代并不影响生长激素通过它而诱导的细胞事件，而另外一些氨基酸残基的取代，例如168位的酪氨酸和332位的丝氨酸分别用在其它种属GHR中高度保守的精氨酸和酪氨酸的取代后，则明显地影响着豚鼠GHR介导的GH诱导的生理效应。

Specifically, the estimate of Lipschitz constant is often conservative.

特别地，估算Lipschitz常数经常太过於保守。

In this paper, firstly, the new combined error model of cumulative geoid height influenced by three error sources including the inter-satellite range-rate of K-band ranging system, orbital position of GPS receiver and non-conservative force of accelerometer from GRACE satellites is established using the semi-analytical method. Secondly, the dependability of error models is demonstrated based on matching relationship among the accuracy indexes of key payloads. Finally, the accuracy of global gravitational field up to degree and order 120 is effectively and rapidly estimated from GRACE Level 1B measured observation errors of the year 2006 publicized by the Jet Propulsion Laboratory in the USA, and the cumulative geoid height error is 18.368 cm at degree 120, which preferably accords with the Earth's gravitational field model EIGEN-GRACE02S provided by the GeoForschungsZentrum Potsdam in Germany.

首先基于半解析法建立了新的GRACE卫星K波段测量系统星间测速、GPS接收机轨道位置和加速度计非保守力误差联合影响累计大地水准面的误差模型；其次，基于各关键载荷精度指标的匹配关系，论证了误差模型的可靠性；最后，基于美国喷气动力实验室公布的2006年的GRACE Level 1B实测误差数据，有效和快速地估计了120阶全球重力场的精度，在120阶处累计大地水准面的精度为18.368 cm，其结果和德国地学研究中心公布的EIGEN-GRACE02S全球重力场模型符合较好。

A strict, conservative teacher is lured by three of his beautiful, nubile young female students into a compromising situation.

一个严厉保守的老师被三个年轻貌美的女学生所勾引，从而开始一步步地妥协。

My view is too conservative, they completely subvert my point of view, in the past I have no obscurely contempt for the famous chef Gusteau "Everyone can be cooking," but now I really understand his meaning.

我的看法太保守了，他们彻底颠覆了我的观点，过去我毫不隐晦地蔑视Gusteau大厨的名言"人人皆可烹饪"，但直到现在我才真正理解他的意思。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

The university said it had decided Rosemary Radford Ruether was the wrong person for the prestigious post because of her service on the Board of Directors of the abortion-rights organization Catholics for Choice.

这是个两面不讨好的决定，当初自从通过聘任萝斯玛利路得为该校资深荣誉教席之后，圣地牙哥大学便不断地受到来自保守天主教集团的压力。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失