保守分子的

Moreover, CoI gene was more conservative and a lower evolutionary rate than Cyt b gene. CoI gene was an effective marker in analysing the phylogenesis of families in Passeriformes. It can be used in identifying the species of Passeriformes, but it was revealed that CoI gene was more suitable to identify the phylogenetic relationship of avian family unit than Cyt b gene, and CoI gene can be a molecular marker to identify avian species. But in species identification, CoI gene was less stable and accurate than Cyt b gene. We suggested youd better employ the other marker if you have the CoI gene only.(2) In the phylogenetic trees of birds from Lanius, L.

比较分析了雀形目6科15种鸟类的细胞色素b全序列和CoI基因部分序列，结果显示细胞色素b和CoI基因序列的变异位点分别为454个和366个、简约信息位点为337个和303个，而且线粒体CoI基因比细胞色素b基因略微保守、进化速率也较低；CoI基因在确定雀形目科级阶元之间的系统发生关系方面是一种有效的分子标记，同时它也能够用于雀形目鸟类的物种鉴定，但在物种鉴别方面不如细胞色素b基因稳定、准确。

Therefore, the LsHNXs genes were cloned in this study, and their function was studied by RNAi. The main results were shown as follows:1 The conservative sequences of three LsHNXs genes (LsHNX1, LsHNX2, and LsHNX3), and the 3'-terminal specific sequences of LsHNX2 and LsHNX3, were cloned by RT-PCR and 3'-RACE.2 The plant RNAi vectors were constructed and the transformation of Limonium sinense was done. The RNAi lines of three single-genes and one double-gene were obtained and were propogated rapidly for the molecular and the stress tolerance analysis.3 To be compared with wild type, the Na+ and K+ contents secreted by the leaves of LsNHX1 transgenic plants were remarkably higher.

主要实验结果如下：1采用RT-PCR和3'-RACE方法从补血草中克隆获得LsNHXs基因家族三个成员LsNHX1、LsNHX2和LsNHX3的保守区序列以及LsNHX2和LsNHX3的3'端特异序列。2构建植物基因RNAi沉默表达载体并转化补血草，获得三个单基因和一个双基因的RNAi沉默表达植株，快繁转基因植株供分子鉴定和耐逆分析。3LsNHX1转基因植株叶片表面分泌物中的Na+、K+离子含量比野生型对照显著增加；叶组织内Na+离子含量和K+离子含量均略高，但差异不显著；K+/Na+比没有显著差异。4LsNHX2转基因植株叶片表面分泌物中的Na+离子含量比野生型对照有所下降，但差异不显著；K+离子含量比对照显著降低。

From the phylogenetic tree constructed for IGF-I, the homology was as high as 95%, which revealed the relative relation between them was more closely than with others and give molecular biology support for the resolution of position and classification of lesser panda.

利用IGF-Ⅰ基因构建的进化树上，大熊猫和小熊猫此基因的同源性达到95%，并且显示小熊猫与大熊猫的亲缘关系与其它哺乳动物相比要近的多，为大熊猫及小熊猫的分类学提供了分子生物学的依据，但这样的结果也许与IGF-Ⅰ在物种间的高度保守性有关，我们认为，对于大小熊猫是否同属一科还需要进一步的研究。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。