保存的

Hx-Ⅰ solution was used in this study to determine its effects in long- term kidney preservation and to compare its performance with HC-WCU kidney preservation solution in autotransplant model.

我们在过去华西Ⅰ号液有效保存肝脏24小时，保存胰腺48小时的基础上，以高渗枸橼酸肾保存液为对照，采用狗单侧自体肾移植模型观察其对狗肾脏保存效果。

The six authors have displayed, in their own ways, a panorama of modem Chinese social life. Zhan Xin-xin expresses the anxiety and lost self of Chinese people at the beginning of main current political culture breakdown and materialistic flooding immediately after the Great Cultural Revolution. Liu Suo-la exposes the anti-classic and pro-individualistic radicalism of young college students facing an opening-up and multi-cultural age. Chen-Rong, in transcendence of historical rationalism, proposes human spiritual emancipation and subjective revelation in order to oppose traditional feudalistic stereotypes. Liu Zhen-yun in his typical cool-minded description, discloses the routine spiritual enslavement, oppression and murder, as well as men\'s helplessness, hopelessness and distortedness in familiar daily life affairs. Zhang Wei attaches importance to a heart-felt emotional and conscientious expression, thinking that the only things that may combine one\'s individual subjective options with his personal moral responsibility towards world and others are his inner authentic feelings and conscience.

这六位作家是张辛欣、刘索拉、谌容、刘震云、张炜和潘军，他们从不同的角度，以各自不同的方式，展示了中国现代社会中的一幅幅人生图景：张辛欣的作品呈现了"文化大革命"以后主流政治文化分化瓦解和物质主义泛滥初始人的自我迷失与彷徨焦虑的状态；刘索拉表达了改革开放和文化多元时代青年大学生反叛经典和张扬个性的激进姿态；谌容从超越历史理性的高度致力于反抗传统意识和封建世俗观念对人格个性的压抑，提倡人的精神解放和主体意志的彰显；刘震云以近乎残忍的描写，揭露了熟视无睹的日常生活对人的精神上的奴役、蹂躏和扼杀以及人在这一过程中的无奈、绝望和扭曲；张炜强调一种内在真情与良知的表达，认为唯一能够把个人的主观选择与对世界、对他人的道德责任联系起来的，就是人在心灵深处为自己和他人保存的那一份真情与良知；潘军从复归自我和维护个性尊严的角度，把人的爱欲和物欲升华为一种超越世俗伦理道德的纯个人化、精神化的心理体验和情感交流。

The six authors have displayed, in their own ways, a panorama of modem Chinese social life. Zhan Xin-xin expresses the anxiety and lost self of Chinese people at the beginning of main current political culture breakdown and materialistic flooding immediately after the Great Cultural Revolution. Liu Suo-la exposes the anti-classic and pro-individualistic radicalism of young college students facing an opening-up and multi-cultural age. Chen-Rong, in transcendence of historical rationalism, proposes human spiritual emancipation and subjective revelation in order to oppose traditional feudalistic stereotypes. Liu Zhen-yun in his typical cool-minded description, discloses the routine spiritual enslavement, oppression and murder, as well as men"s helplessness, hopelessness and distortedness "in familiar daily life affairs. Zhang Wei attaches importance to a heart-felt emotional and conscientious expression, thinking that the only things that may combine ones individual subjective options with his personal moral responsibility towards world and others are his inner authentic feelings and conscience.

这六位作家是张辛欣、刘索拉、谌容、刘震云、张炜和潘军，他们从不同的角度，以各自不同的方式，展示了中国现代社会中的一幅幅人生图景：张辛欣的作品呈现了"文化大革命"以后主流政治文化分化瓦解和物质主义泛滥初始人的自我迷失与彷徨焦虑的状态；刘索拉表达了改革开放和文化多元时代青年大学生反叛经典和张扬个性的激进姿态；谌容从超越历史理性的高度致力于反抗传统意识和封建世俗观念对人格个性的压抑，提倡人的精神解放和主体意志的彰显；刘震云以近乎残忍的描写，揭露了熟视无睹的日常生活对人的精神上的奴役、蹂躏和扼杀以及人在这一过程中的无奈、绝望和扭曲；张炜强调一种内在真情与良知的表达，认为唯一能够把个人的主观选择与对世界、对他人的道德责任联系起来的，就是人在心灵深处为自己和他人保存的那一份真情与良知；潘军从复归自我和维护个性尊严的角度，把人的爱欲和物欲升华为一种超越世俗伦理道德的纯个人化、精神化的心理体验和情感交流。

Test teaching results of many years proved that comprehensive application,such as field collection,marcket purchase,natural cultivation,simple inoculation and other materials for preparing slides and materials collection methods as well as temporary preservation,dry preservation at room temperature,cryodrying preservation and cryopreservation,could meet the need of slide making of fungus,bacterium,virus,nemathelminth and other typical disease in a long term.

多年的实验教学证明，综合应用田间采集、市场购买、自然培养、简易接种等制片材料采集方法与临时保存、室温干燥保存、低温干燥保存、冷冻保存等保存技术，可以长期满足真菌性病害各亚门、细菌性、病毒性及线虫性等典型病害制片材料的需要。

This essay explores using micosatellite DNAs to study the genetic diversity, and its major contents are as follows:The contrast among different methods of feces preservation: The methods are that feces was respectively stored in the field during collection at a low temperature after drying at 60 ℃, stored in 75% ethanol, stored in 100% ethanol and directly stored at a low temperature(in the attemperator added the biological ice bag),The result showed that DNA could be extracted successfully from the feces in a short storing period with any of these methods.

本文应用微卫星标记来研究普氏原羚遗传多样性，主要研究内容如下：一、不同普氏原羚粪便保存方法比较：通过将普氏原羚的粪便在野外分别采取60℃干燥后低温保存，75％乙醇保存，100％乙醇保存，直接低温保存。

Three weeks afer the allografting,there was no significant difference between the two al lografting groups in maximum load(P＞0.05),Eight weeks later,the maximum load of the group that was preserved by liquid nitrogen after program freezing(76.60±4.67)N was better than the group that was preserved by －80℃ deepfrozen(75.99±4.20) and similar with the autografting group(76.72±2.55).4.The healing process and histological behavior of the two deepfrozen groups are similar with the autografting group,and the program freezing group is better.

结果 a经程序冷冻液氮保存方法处理后，髌腱的最大载荷无明显下降，细胞活性得到了较好的保存，组织学观察冷冻损伤较－80℃深低温保存方法轻微；b程序冷冻液氮保存处理的移植物在术后未表现明显的排斥反应，且免疫反应随时间的推移而下降；c移植后3周，各组移植物的最大载荷无显著差异(P＞0.05)，移植后8周，程序冷冻液氮保存组移植物的最大载荷(55.87±1.86)N优于－80℃深低温保存组(52.14±2.79)，而和自体移植组相近(57.70±2.76)N；d从组织学观察看，－80℃深低温保存组和程序冷冻液氮保存组移植后的愈合过程均和自体移植组相似，而程序冷冻液氮保存组的愈合过程和组织学行为更接近于自体移植组。

Results 1.After treated by:program freezing,the maximum load of the allografts has no evident decrease and the cell activity of allografts was preserved well,the frozen hurt after program freezing was more slight than －80℃ deepfrozen,2.No evident reject reaction can be seen during the healing process after deep frozen and the reject reaction declined as the time pass by.3.Three weeks afer the allografting,there was no significant difference between the two al lografting groups in maximum load(P＞0.05),Eight weeks later,the maximum load of the group that was preserved by liquid nitrogen after program freezing(76.60±4.67)N was better than the group that was preserved by －80℃ deepfrozen(75.99±4.20) and similar with the autografting group(76.72±2.55).4.The healing process and histological behavior of the two deepfrozen groups are similar with the autografting group,and the program freezing group is better.

结果 a经程序冷冻液氮保存方法处理后，髌腱的最大载荷无明显下降，细胞活性得到了较好的保存，组织学观察冷冻损伤较－80℃深低温保存方法轻微；b程序冷冻液氮保存处理的移植物在术后未表现明显的排斥反应，且免疫反应随时间的推移而下降；c移植后3周，各组移植物的最大载荷无显著差异(P＞0.05)，移植后8周，程序冷冻液氮保存组移植物的最大载荷(55.87±1.86)N优于－80℃深低温保存组(52.14±2.79)，而和自体移植组相近(57.70±2.76)N；d从组织学观察看，－80℃深低温保存组和程序冷冻液氮保存组移植后的愈合过程均和自体移植组相似，而程序冷冻液氮保存组的愈合过程和组织学行为更接近于自体移植组。

On a consultation held in our printing-office among his friends, what he should do in this conjuncture, it was proposed to elude the order by changing the name of the paper; but my brother, seeing inconveniences in this, came to a conclusion, as a better way, to let the paper in future be printed in the name of BENJAMIN FRANKLIN; and in order to avoid the censure of the Assembly, that might fall on him as still printing it by his apprentice, he contrived and consented that my old indenture should be returned to me, with a discharge on the back of it, to show in case of necessity; On a consultation held in our printing-office among his friends, what he should do in this conjuncture, it was proposed to elude the order by changing the name of the paper; but my brother, seeing inconveniences in this, came to a conclusion, as a better way, to let the paper in future be printed in the name of BENJAMIN FRANKLIN; and in order to avoid the censure of the Assembly, that might fall on him as still printing it by his apprentice, he contrived and consented that my old indenture should be returned to me, with a discharge on the back of it, to show in case of necessity; and, in order to secure to him the benefit of my service, I should sign new indentures for the remainder of my time, which was to be kept private.

他的许多朋友，在印刷所中开会讨论，这件案子该怎样办。有人建议改变名称来规避这个命令；但是我的哥哥认为这个方法不大合适，最后决定一种较好的办法，让未来的这张报刊发行时用拜雅明富兰克林的名字；但是为避免议会的责罚，如果仍旧以他的学徒刊行还是不行，最好办法是暗地里把旧合约给我，合约的后面注明作废，这样一旦有事就可以给人家看了。他的许多朋友，在印刷所中开会讨论，这件案子该怎样办。有人建议改变名称来规避这个命令；但是我的哥哥认为这个方法不大合适，最后决定一种较好的办法，让未来的这张报刊发行时用拜雅明富兰克林的名字；但是为避免议会的责罚，如果仍旧以他的学徒刊行还是不行，最好办法是暗地里把旧合约给我，合约的后面注明作废，这样一旦有事就可以给人家看了。但是为保护我为他工作的利益，剩下的年限我再签一张新合约，这是私自保存的。这是一个下等的计策；但不论怎样，这个计策立即执行了，而这份报依此刊行，用我的名字已有几个月了。

Cell embryos were cultured in vitro, then viability was observed. The results showed the fertilizing rate in vitro of the group for GV oocytes with cumulus cells after maturating which were conserved at 10℃ for 24 h, 15℃ for 14 h, 20℃ for 8 h and 25℃ for 4 h was 14%, 9%, 10% and 10% respectively, and with the advance of storage temperature and extension of storage time, the rate of 2-cell after maturating-fertilizating of the GV oocytes decreased significantly.

其结果，在10℃下保存24h、15℃下保存14h、20℃下保存8h和25℃下保存4h后，其体内附有卵丘细胞的GV卵的体外成熟－体外受精后的2细胞率分别为14%、9%、10%和10%，随着保存温度的提高和保存时间的延长，带有颗粒细胞GV期卵的比率明显降低，同时其GV期卵经体外成熟及体外受精后的2细胞率明显降低。

Methods The purified general bacteria in broth culture for46hourswerepreserved in glycerol-physiological saline,the purified streptococcus and Neisseria scraped from the agar culture were preserved in fresh sheep-blood which was added suitable glycerol-physiological saline,the purified fungi were cultivated in half-solid culture;-20℃general bacteria and fungi,-80℃streptococcus and Neisseria.

将普通细菌的菌种纯化后接种于普通肉汤管，4～6h增菌后，加入适量甘油-生理盐水保存液，-20℃冰箱保存；链球菌属和奈瑟氏菌属的菌株纯化后，用接种环取菌置于新鲜脱纤维绵羊血中，加入适量保存液，-80℃低温冰箱保存；真菌菌种纯化后接种于带硅胶塞的半固体培养基，25℃培养24h后，-20℃冰箱保存。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。