保存

GV oocytes with normal shape were not obtained in the group which was stored at 20℃ for 24 h and at 25℃ for 14 h; A blastula rates (64%) of all groups in average was gotten in culturing 2-cell in vitro, and there is no significant difference in temperature and time of storage. After transplantating some of the 2-cell embryos which were obtained from GV oocytes stored at 15'C for 8 h, normal infant mice could be obtained.

在20℃下保存24h和25℃下保存14h时，难以获得形态正常的GV期卵；体外受精获得的2细胞期胚经体外培养，总体上有64%的胚胎发育至扩张囊胚，未见有保存温度和保存时间的显著影响，且利用在15℃保存8h后的GV卵获得2细胞期胚的移植获得正常新生小鼠。

The first part consists of three experiments:(1) The rings were incubated in KH, 20, 50 mmol/L 〓 for 1 house, relaxation in response to the EDHF stimuli A23187 in 30nmol/L U46619-induced preconrtaction in the presence of 7 μ mol/L indomethacin, a cyclooxygenase inhibitor, 300μmol/L LNNA, a nitric oxide biosyhnthesis inhibitor, and 1mmol/L tetraethylammonium , a 〓 blocker, or 3 μmol/L glibenclamide , a 〓 blocker, was compared with the control;(2) After the arteries were incubated in KH, UW solution or HTK solution at 4℃ for 4 hours, endothelium-derived relaxation (percentage of 30nmol/L U46619 precontraction) was induced by A23187 in the present of 7 μmol/L indomethacir and 300μmol/L LNNA;(3) After incubation with KH, UW solution and STH (either at 37℃ in oxygenated organ chamber or at 4℃ in a refrigerator for 4 hours), endothelium-derived relaxation (percentage of 30nmol/L U46619 precontraction) was induced by A23187 in the present of 7 μ mol/L indomethacin and 300 μmol/L LNNA.

第一部分研究结果：（1）单纯浸泡于KH的冠状动脉A23187能引发66.67％的血管舒张反应，经TEA及20mmol／L、50mmol／L钾离子作用后，血管舒张反应程度显著降低，但经GBM作用后改变不明显；（2）与保存于KH的冠状动脉相比，A23187引发的血管舒张反应程度，保存于UW液的明显下降，保存于HTK液的无明显变化；（3）在37℃条件下，血管环浸泡于STH出现缓慢轻微的舒张反应，浸泡于UW液初期出现短暂收缩反应，但此后主要以舒张为主：在37℃条件下，血管经UW液保存后，U46619引发的收缩反应程度降低；不论在37℃或4℃条件下，A23187引发的血管舒张反应，经UW液保存后明显下降，但用STH保存后变化不明显。

Monitor Memories are made of this 记忆是这样炼成的 Sep 3rd 2009 From The Economist print edition Computing: Memory chips based on nanotubes and iron particles might be capable of storing data for a billion years 计算机:毫微管和铁粒子造存储芯片,数据保存能达十亿年 FEW human records survive for long, the 16,000-year-old Paleolithic cave paintings at Lascaux, France, being one exception.

人工记录很少能长期保存。

Test teaching results of many years proved that comprehensive application,such as field collection,marcket purchase,natural cultivation,simple inoculation and other materials for preparing slides and materials collection methods as well as temporary preservation,dry preservation at room temperature,cryodrying preservation and cryopreservation,could meet the need of slide making of fungus,bacterium,virus,nemathelminth and other typical disease in a long term.

多年的实验教学证明，综合应用田间采集、市场购买、自然培养、简易接种等制片材料采集方法与临时保存、室温干燥保存、低温干燥保存、冷冻保存等保存技术，可以长期满足真菌性病害各亚门、细菌性、病毒性及线虫性等典型病害制片材料的需要。

This essay explores using micosatellite DNAs to study the genetic diversity, and its major contents are as follows:The contrast among different methods of feces preservation: The methods are that feces was respectively stored in the field during collection at a low temperature after drying at 60 ℃, stored in 75% ethanol, stored in 100% ethanol and directly stored at a low temperature(in the attemperator added the biological ice bag),The result showed that DNA could be extracted successfully from the feces in a short storing period with any of these methods.

本文应用微卫星标记来研究普氏原羚遗传多样性，主要研究内容如下：一、不同普氏原羚粪便保存方法比较：通过将普氏原羚的粪便在野外分别采取60℃干燥后低温保存，75％乙醇保存，100％乙醇保存，直接低温保存。

The study indicated that the effects of self-designed KYL solution on rat liver preservation are similar to that of UW solution.

结果 KYL液保存的大鼠肝脏在保存16 h以内各时相胆汁流出量均较UW液保存者高（P＜0.01，灌注流出液AST、ALT和LDH含量与UW液保存者相近，肝细胞内钙离子浓度较UW液保存者低（P＜0.01； KYL组保存24及48 h时，MDA含量低于UW组，SOD含量高于UW组（P＜0.01）；光、电镜观察两者形态学变化基本一致。

Three weeks afer the allografting,there was no significant difference between the two al lografting groups in maximum load(P＞0.05),Eight weeks later,the maximum load of the group that was preserved by liquid nitrogen after program freezing(76.60±4.67)N was better than the group that was preserved by －80℃ deepfrozen(75.99±4.20) and similar with the autografting group(76.72±2.55).4.The healing process and histological behavior of the two deepfrozen groups are similar with the autografting group,and the program freezing group is better.

结果 a经程序冷冻液氮保存方法处理后，髌腱的最大载荷无明显下降，细胞活性得到了较好的保存，组织学观察冷冻损伤较－80℃深低温保存方法轻微；b程序冷冻液氮保存处理的移植物在术后未表现明显的排斥反应，且免疫反应随时间的推移而下降；c移植后3周，各组移植物的最大载荷无显著差异(P＞0.05)，移植后8周，程序冷冻液氮保存组移植物的最大载荷(55.87±1.86)N优于－80℃深低温保存组(52.14±2.79)，而和自体移植组相近(57.70±2.76)N；d从组织学观察看，－80℃深低温保存组和程序冷冻液氮保存组移植后的愈合过程均和自体移植组相似，而程序冷冻液氮保存组的愈合过程和组织学行为更接近于自体移植组。

Results 1.After treated by:program freezing,the maximum load of the allografts has no evident decrease and the cell activity of allografts was preserved well,the frozen hurt after program freezing was more slight than －80℃ deepfrozen,2.No evident reject reaction can be seen during the healing process after deep frozen and the reject reaction declined as the time pass by.3.Three weeks afer the allografting,there was no significant difference between the two al lografting groups in maximum load(P＞0.05),Eight weeks later,the maximum load of the group that was preserved by liquid nitrogen after program freezing(76.60±4.67)N was better than the group that was preserved by －80℃ deepfrozen(75.99±4.20) and similar with the autografting group(76.72±2.55).4.The healing process and histological behavior of the two deepfrozen groups are similar with the autografting group,and the program freezing group is better.

结果 a经程序冷冻液氮保存方法处理后，髌腱的最大载荷无明显下降，细胞活性得到了较好的保存，组织学观察冷冻损伤较－80℃深低温保存方法轻微；b程序冷冻液氮保存处理的移植物在术后未表现明显的排斥反应，且免疫反应随时间的推移而下降；c移植后3周，各组移植物的最大载荷无显著差异(P＞0.05)，移植后8周，程序冷冻液氮保存组移植物的最大载荷(55.87±1.86)N优于－80℃深低温保存组(52.14±2.79)，而和自体移植组相近(57.70±2.76)N；d从组织学观察看，－80℃深低温保存组和程序冷冻液氮保存组移植后的愈合过程均和自体移植组相似，而程序冷冻液氮保存组的愈合过程和组织学行为更接近于自体移植组。

Cell embryos were cultured in vitro, then viability was observed. The results showed the fertilizing rate in vitro of the group for GV oocytes with cumulus cells after maturating which were conserved at 10℃ for 24 h, 15℃ for 14 h, 20℃ for 8 h and 25℃ for 4 h was 14%, 9%, 10% and 10% respectively, and with the advance of storage temperature and extension of storage time, the rate of 2-cell after maturating-fertilizating of the GV oocytes decreased significantly.

其结果，在10℃下保存24h、15℃下保存14h、20℃下保存8h和25℃下保存4h后，其体内附有卵丘细胞的GV卵的体外成熟－体外受精后的2细胞率分别为14%、9%、10%和10%，随着保存温度的提高和保存时间的延长，带有颗粒细胞GV期卵的比率明显降低，同时其GV期卵经体外成熟及体外受精后的2细胞率明显降低。

This article introduced based on the Labview8.0hypothesized sine, the cosine, the square-wave, the saw-tooth wave,the triangle wave signal generating device realization May accordingto the need, the change profile frequency and the peak-to-peak value,preserves the profile the analysis parameter to assign the document,and introduced based on the USB data acquisition card dummy signaloutput LabVIEW,虚拟仪器,保存数据,信号发生器 LabVIEW, the hypothesized instrument, preserves the data, the signalgenerating device

本文介绍了基于Labview8.0的虚拟正弦，余弦，方波，锯齿波，三角波信号发生器的实现。可以根据需要，改变波形的频率和幅值，保存波形的分析参数到指定文件，并介绍了基于USB数据采集卡的虚拟信号输出。关键字：LabVIEW，虚拟仪器，保存数据，信号发生器问题补充：请不要直翻，中国式英语我不要。逐词翻译组成的句子我也会。我只要正确的翻译。不是正确回答不要打扰

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。