促进作用

He type of ossein in group B was mainly type Ⅰ collagen while that in group A type Ⅱ and it exhibited as a process of fiber formation in group C. The reason for why TGF-β〓 can promote ossification induced by BMP is that can promote the differentiation and proliferation of mesenchymal cells to supply more target cells for BMP, promote the differentiation and proliferation of mature osteocytes and chondrocytes, inhibit metalloproteinase to decrease the destruction of bone tissue and promote calcification of bone matrix.

GF-〓能促进BMP骨诱导作用主要因其能促进间充质细胞的分裂增殖为BMP提供丰富的靶细胞、促进成熟骨及软骨细胞的分裂增殖、促进骨细胞产生Ⅰ型胶原而抑制软骨细胞产生Ⅱ型胶原，抑制金属蛋白酶的活性以减少组织破坏、促进基质钙化等作用而使骨生成能力增加，提示它和BMP具有协同作用。

We can mak use of the combination of two policy. Suggestions: In order to improve RUD in rural areas of China and RUD researches, the researcher suggested: 1.Pay more attention to the manager's impact on improving RUD and make use of its paradigm and leading impact. 2.Apply ten core interventions including STG and EDL. They are applying STG, applying EDL, developing a drug management department and perfecting its function, educating health workers, supervising and checking up the use of drugs, developing independent drug information system, carrying out public education, economic interventions, improving the work rate of drug use and health workers by ensuring sufficient budget. 3. Make use the pressure of applying the new-type CMS and apply many types of interventions to improve RUD in rural areas.

建议：为进一步促进农村地区合理用药，完善农村地区合理用药研究，建议： 1、重视乡镇卫生院院长在促进合理用药中的作用，充分发挥其示范、带动作用； 2、实施以临床诊疗规范、基本药物目录为主的十条核心干预措施，具体包括：实施临床诊疗规范、实施基本药物手册、建立医院药事管理机构，完善相关职能、开展医务人员培训、开展用药督导和审核、建立独立的药物信息提供体系、开展药物的公共教育、经济措施、保证政府投入以提高用药和医务人员的工作效率； 3、利用实施新型农村合作医疗制度的压力机制，综合使用多种措施协同促进合理用药； 4、多部门协作共同促进农村地区合理用药，由合作医疗和医疗救助等保障制度管理部门、财政部门、卫生行政部门、药品监管部门、工商行政部门、物价部门、媒体等共同参与，各司其责，共同促进农村地区合理用药； 5、在合理用药评价研究中应密切注意农村地区药品利用不足问题，建立正确的评价假设； 6、构建适合我国国情的完善的合理用药干预措施效果评价指标体系，加强标准值研究。

The mechanisms of these actions may be involved in anti-exitotoxicity of aminoglutaric acid, regulating the synthesis of nitric oxide, antioxidation, antiinflammatory, inhibiting neuron apoptosis, accelerating angiogenesis, neurogenesis, neurotrophy and others.

其作用机制可能包括抗谷氨酸兴奋毒性、调节NO的合成、抗氧化作用、抗炎作用、抗神经元凋亡、促进血管生成、促进神经元再生和神经营养作用等。

DCs acquired by our reformed methods express both CD83 and CD 14 molecules highly, and have a higher density than other domestic reports. The higher TNF in DCs culture medium of HC patient suggests DCs in patient still have antigen presenting ability and by optimization the culture medium would improve its presenting ability and have a potential value in design and application individual vaccine. Although antigens pulsed DCs have a decrescent antigen presenting ability but BCG HSP70 could induce its mature and improve its presenting ability. Suggests BCG HSP70 would be a useful mature inducer. Lymphocytes primed by DCs based HC vaccine have the specific cytotoxicity against HCC lines. The CTL after freezing and anabiotic could prophylaxis and therapy HC xenograft on nude mouse. The results also suggests that CD4〓 lymphocytes play a important role in HC with a good differentiation and would be useful in treatment this kind of HC. After being activated by Peptide LLNQHACAV of hAFP and apoptotic HCCs pulsed DCs respectively, the culture medium of activated lymphocytes both contains a high level Th1 cytokines IL-12 and TNF. Primed lymphocytes appeared a characteristic of NK cells. DCs not only inhibited the growth of human HCC and other cancer cells in vitro but also prevented the growth of HC xenograft on nude mouse in vivo. There are at least three kinds of mechanism playing important role in DC based vaccine ,there are inhibition of DCs, HC specific CTL and cytokines pathway.

诱导出的DC共同表达CD83和CD14分子，CD83分子表达明显高于国内报道；肝癌患者DC培养上清中TNF水平高于健康人，提示肝癌患者DC仍具一定的抗原呈递能力，适当调控可使其行使APC功能，以期在肝癌个体化疫苗中发挥作用；DC负载肝癌可溶性抗原后，抗原呈递能力降低，BCG HSP70可促进DC成熟，增加其抗原呈递能力，预示BCG HSP70有可能成为促进DC活化和成熟的另一重要分子；肝癌DC疫苗在体外诱导肝癌特异性淋巴细胞，活化的淋巴细胞在体外对肝癌细胞的杀伤以特异性CTL为主，同时分泌较高水平Th1型细胞因子IL-12和TNF，并抑制4种肝癌细胞生长；冷冻复苏后的肝癌特异性淋巴细胞可以预防和抑制人肝癌裸鼠皮下移植瘤，提示DC负载肝癌可溶性抗原后诱发的MHC-Ⅱ类限制性CD4〓T细胞有可能在分化程度高的肝癌治疗中发挥作用；用DC和HLA-A2〓DC分别负载凋亡肝癌细胞和hAFP218-226位LLNQHACAV HLA-A2限制性九肽，在体外诱导肝癌特异性淋巴细胞，活化后的CTL细胞分泌较高水平的Th1型细胞因子IL-12和TNF，并具较强杀伤活性，此CTL同时具备NK细胞特征；DC对肿瘤细胞的抑制作用可能是通过吞噬实现的，Fas-L在DC抑制中也起一定作用；DC对人肝癌裸鼠皮下移植瘤的抑制率为97％；在肝癌DC疫苗的作用中，至少联合3种以上机制，即通过DC的直接作用、肝癌特异性CTL和细胞因子途径直接或间接地杀伤和抑制肝癌细胞。

The esterifying effects of monascus, daqu and rhizopus strain on single acid (acetic acid, caproic acid, lactic acid, butyric acid) and mixed acid (acetic acid,caproic acid,lactic acid, and butyric acid mixed together according to the ratio of 1∶1∶1∶1) were studied.

对红曲霉、大曲以及根霉菌对单一酸（乙酸、己酸、乳酸、丁酸）和混合酸（四大酸按1∶1∶1∶1混合）的酯化效果进行了研究，结果表明，红曲霉能促进己酸、丁酸及混合酸与乙醇的酯化作用，生成的酯类物质均为己酸乙酯，而且酯化能力极强；大曲能促进己酸、丁酸及混合酸与乙醇的酯化作用，生成的酯类物质均为己酸乙酯，并且能促进乙酸与乙醇的酯化生成乙酸乙酯；根霉能促进己酸和混合酸与乙醇的酯化作用，生成少量的己酸乙酯和乳酸乙酯。

PartⅡThe mechanism elucidation about effects ofα-(2,3)/(2,6)sialic acid on the Cx43gap-junction functions.(1) Westernblotting experiment showed that the decrease of sialic acid didn\'t changethe Cx43 expression and its phospholation level.(2) Westernblotting experiment showed sialidase didn,t change the ZO-1 expression,IP and confocal experiment showed sialidase improved the interaction of Cx43 andZO-1.(3) Westernblotting experiment showed sialidase didn\'t change N-cadherin expression,IP and confocal experiment showed sialidase promoted the complex formation ofCx43 and N-Cadherin.(4)Sialidase could increase the ERK1/2 phospholation level,and enchanedintercellular homotypic adhesion,Immunofluorometric assay showed sialidase couldpromote the N-cadherin cluster on the membrance.

第二部分：α—(2，3)／(2，6)唾液酸对肿瘤细胞CX43间隙连接功能影响的机制研究1、Westernblotting结果表明降低细胞膜表面唾液酸并不改变Cx43的表达及其磷酸化水平：但Cx43连接斑形成增多；2、Westernblotting结果表明唾液酸酶作用后细胞ZO—1表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与ZO-1的结合；3、Westernblotting结果表明唾液酸酶作用后细胞N-cadherin表达没有改变，IP及免疫荧光共定位结果表明唾液酸酶作用后促进了Cx43与N-Cadherin复合物的形成；4、唾液酸酶作用后细胞内ERK1／2磷酸化水平明显增加，细胞间同质粘附增加，以及免疫荧光表明唾液酸降低后可促进N-cadherin的膜成簇。

The dynamic changes of the the neuron apoptosis and correlative genes in hippocampus of ACET-groups and twice-ACET-groups suggested that ACET can slower the apoptosis by inhibiting p~(53)and prolong the ascending period of p~(53) in 4 days after the seizure,this effect would weaken after 4 days.ACET may inhibit the descending of Bax,prolong its descending period and then promote its expression,in order to weaken the inhibition of the apoptosis during 4 to 10 days after seizure and then promote the apoptosis during 10 to 14 days after.This effect of ACET can be enhanced after treating again within 7 to 14 days later.

埋线组和二次埋线组大鼠海马神经元凋亡及相关基因表达的动态变化结果提示：埋线治疗在癫痫发作后的早期(4d内)可通过抑制促凋亡基因p~(53)蛋白表达、延缓其达峰值的时程，从而减缓神经元凋亡的发生，此作用在4d后减弱；埋线治疗抑制大鼠海马神经元凋亡的作用在癫痫发作后的中期(4d～10d)减弱，甚至在远期(10d～14d)出现促进凋亡的作用，可能是通过抑制Bax蛋白表达的下降、延长其降至低峰值的时程，促进Bax蛋白表达的回升的结果；在中远期(7d～14d)内予二次埋线治疗对该效应有续效叠加的作用。

The results demonstrated that laurocapram disrupts the highly ordered lipid structure of stratum comeum and makes the structurs of SC loose,which causes the outermost cell of SC easy to exfoliate and decreases the barrier function of skin for the transdermal absorption of drug;Meawhile, laurocapram penetrating into the intracellular matrix of SC cells fluidizes the filaggrin matrix protein and swells the dermal SC, which could increase the free volume of drug and water content in the SC.

探讨氧酮促进洛美沙星透皮吸收的作用机理。结果表明，氮酮通过以下途径促进药物透皮吸收：（1）作用于表皮角质层细胞间脂质。使角质层变得疏松。细胞间距增大。外角质层细胞易于脱落。降低了皮肤对药物的屏障作用；（2）氮酮进入角质细胞内。与细胞内基质相作用。引起基底角质层肿胀。

Which can be used as the body of vitamin B1 coenzyme of many enzymes in the normal human body has an important role in glucose metabolism, it can inhibit the activity of cholinesterase, and choline esterase is an important neurotransmitter, and thus nerve conduction impact; vitamin B2 in the organization by participating in a variety of yellow enzymes constitute a coenzyme, its role in bio-oxidation process in the delivery of hydrogen role in regulating the body's metabolism process; vitamin B6 can also be a variety of enzymes as coenzyme participate in all kinds of amino acid metabolism, promote the absorption of amino acids and protein synthesis, cell growth is necessary for the material, which also promote fat metabolism, involved in neurotransmitter synthesis, hemoglobin synthesis and the impact on the normal reproductive functions such as human body an important metabolic process, Volkswagen Medicine Net: What liver protection effect of vitamin B family?

可作为维生素乙许多酶的辅酶，在正常人体组织中有一个用糖代谢的重要作用，能抑制胆碱酯酶活性，和胆碱脂酶是一个重要的神经递质，神经传导，从而影响；由黄色参与各种酶在组织中维生素B2构成辅酶，其作用在生物氧化氢的作用，调节人体的代谢过程中产程；维生素B6也可以是作为辅酶参与氨基酸代谢的各种酶的品种，促进氨基酸的吸收和蛋白质合成，细胞生长是必要的材料，这也促进脂肪代谢的神经递质合成，血红蛋白合成和参与，如影响人体生殖功能的正常新陈代谢过程中一个重要的，（大众医药网：什么样的维生素B家庭肝脏的保护作用？

We have come to a conclusion that, the development of China\'s APPI has visible active influences on promoting the increase of fanners\' per capita income and net income, promoting the growth of industrial output value in rural areas, and promoting the growth of GDP in rural areas.

我们得出：我国农产品加工业的发展对于促进农民人均总收入和纯收入的提高发挥了明显的作用，对于促进农村工业产值的增长发挥了明显的作用，对于促进农村GDP的增长发挥了明显的作用。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。