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The seizure sensitive rats were made by subcutaneous injection of a convulsant dose (10mg/kg) of KA. Normal saline , PL017 or β-FNA were infused constantly into ventral hippocampus of awakened and freely moving animals for 7 days by mini-osmotic pumps. 7 days later, all rats were given subcutaneously with subconvulsant dose of KA.

皮下注射惊厥剂量(10mg/kg)的KA制备颞叶癫癎模型的癫癎发作敏感大鼠,采用微渗透泵技术分别将生理盐水、PL017和β-FNA连续7天恒速注入清醒自由活动的上述动物的腹侧海马内。7天后皮下注射阈下剂量(5mg/kg)KA检测癫癎发作敏感性形成情况。

At approximately 24 hours post-fertilization, fez expression is mostly confined to the telencephalon and the anterior-ventral region of the diencephalon.

受精后24小时的斑马鱼胚胎中,Fez的表达范围主要分布在胚胎的端脑和间脑的腹侧前端。

Objective. To describe 2 cases with spontaneous deep unilateral abdominal pain as the first symptom of thoracic disc herniation at a low thoracic vertebral level, further manifested by unilateral partial paresis of the obliquus abdominis muscle.

目的:描述2例由于下位胸椎间盘突出症以自发深部的腹壁疼痛为首发症状的患者,进一步的检查发现有单侧的腹外斜肌瘫痪。

Using RT-PCR analysis, RGS7 was expressed from fertilization to 96 hours post-fertilization of Zebrafish. Expression of RGS7 was found in brain、aorta ventralis、intermediate cell mass and somite by whole-mount in situ hybridization.

因此,利用RT-PCR以及全胚体原位杂合技术,结果发现在受精后0~96小时的斑马鱼胚胎中皆可侦测到RGS7的表现,其表现的位置是在斑马鱼胚胎的脑部、腹侧主动脉、中期细胞团及体节等组织部位。

But the latent period for reaching the platform above the water in Morris Water Maze of the Mn-exposed group was not different from that of control group. There was a significant increase in the areas,glial fibrillary acid proteinimmunoreactivity and the average proportional densities of GFAP-positive elements in nucleus caudate and accumbens in the high Mn-exposed group, and a significant reduction in the tyrosine hydroxylase immunoreactivity and average proportional densities of TH-positive elements in substantia nigra,ventral tegmentum area of midbrain and nucleus caudate in the high Mn-exposed group.

2高剂量染锰组尾状核和伏隔核的面积、胶质细胞纤维酸蛋白免疫反应强度及反应阳性产物的平均相对密度均较对照组显著升高;(3)高剂量组仔鼠黑质、中脑腹侧被盖区和尾状核的酷氨酸氢氧化酶免疫反应强度及其反应阳性产物的平均相对密度均比对照组明显下降。

There was a significant increase in the areas,glial fibrillary acid proteinimmunoreactivity and the average proportional densities of GFAP-positive elements in nucleus caudate and accumbens in the high Mn-exposed group, and a significant reduction in the tyrosine hydroxylase immunoreactivity and average proportional densities of TH-positive elements in substantia nigra,ventral tegmentum area of midbrain and nucleus caudate in the high Mn-exposed group.

2高剂量染锰组尾状核和伏隔核的面积、胶质细胞纤维酸蛋白免疫反应强度及反应阳性产物的平均相对密度均较对照组显著升高;(3)高剂量组仔鼠黑质、中脑腹侧被盖区和尾状核的酷氨酸氢氧化酶免疫反应强度及其反应阳性产物的平均相对密度均比对照组明显下降。

ABSTRACT Aim and Methods: Using fast cyclic voltammetry and carbon fibre mi croelectrode, we monitored dopamine release from caudate putamen ,nucleus accumbens and central amygdaloid nucleus of normal rats in v ivo, in order to find out the suitable parameters to evoke DA release of certa in area. DA release was evoked by electrical stimulation of medial forebrain bundl e or ventral tegmental area.

目的和方法:采用快速周期伏安法在体研究电刺激内侧前脑束或腹侧背盖区诱发的纹状体、伏核或中央杏仁核多巴胺释放的特点,探索电刺激诱发不同核团DA释放的适宜刺激参数。

Methods: To use ECM Gel as a tissue culture substrate, 3-dimention co-culture of the choroids plexus or the Neuro-2A cells with the ventral part of the embryonic spinal motor column (Embryonic 12 days, E12) were made in the substrate.

采用ECM Gel作为组织培养的基质底物,将脉络丛组织或者Neuro-2A细胞株和胚胎12天的腹侧脊髓运动神经柱进行三维共培养,观察脉络丛组织或者Neuro-2A细胞株对胚胎脊髓运动神经元轴突生长的影响。

At 24 h, Ang1 was expressed mainly in the lens, optic cup, mesencephalon, ventral part of hindbrain, and myotomes in the somites, while zfTie2 was mainly expressed in intersomitic vessels including dorsal aorta and axial veins, and sprouting vessels from the dorsal aorta in the trunk. VEGF was expressed mainly in the lens, optic cup, mesencephalon and myotomes in the somites, while zfFlk1 was mainly expressed in intersomitic vessels including dorsal aorta and axial veins, and sprouting vessels from the dorsal aorta in the trunk.

结果从受精卵胚胎发育第12小时可见血管内皮细胞生长因子和血管发生素1及其相应受体flk1和Tie2的表达;在受精卵胚胎发育的第16小时,血管内皮细胞生长因子和血管发生素1主要在头部和尾部胚芽表达;第24小时在眼球、视窝、中脑、后脑的腹前侧和肌节中表达,Tie2和Flk1主要在肌间背部动脉血管、躯干新生血管芽和轴向静脉中表达。

Standard EMG was recorded at bilateral quadriceps femoris muscles, triangular muscles, sternocleidomastoid muscles and rectus abdominis muscles. Parameters studies included spontaneous activity ; duration, amplitude of polyphasic wave of motor unit action potential; pattern of recruitment.

EMG取双侧股四头肌、三角肌、胸锁乳突肌和腹直肌,检测项目包括静止时的自发电位,小力收缩运动单位动作电位的时限、波幅,大力收缩时的募集相。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

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