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METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity. The culture medium was renewed after 48 hours, and non-adhesive cells were removed.

无菌条件下截取流产胎儿双侧四肢骨,PBS冲洗髓腔,离心去脂肪及上清液,L-DMEM重悬细胞,沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中,离心后吸取中间界面白膜层,加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基,接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液,去除未贴壁细胞,以后每2 d换液一次。

The puerperant and her relatives all signed informed consents. METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity.

无菌条件下截取流产胎儿双侧四肢骨,PBS冲洗髓腔,离心去脂肪及上清液,L-DMEM重悬细胞,沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中,离心后吸取中间界面白膜层,加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基,接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液,去除未贴壁细胞,以后每2 d换液一次。

Methods The gastral wedge of a relatively small width was rotated to the middle of the posterior wall of gastral wedge,the gastroduodenal anastomosis is approximated in Billroth Ⅰ method.175 consecutive patients were treated in this method.Results Less postoperative complications were recorded.

175例胃大部切除术后患者采用胃十二指肠小吻合口,小弯侧瓣向后旋转,使残胃小弯侧瓣口成为后壁之中点,残胃与十二指肠对端吻合的方法。

A structured grid partitionfor full scale coal fired tangential utility boiler has been given in this paper. It hasa small amount of grids and can decrease false diffusion effectively as it is inagreement with fluid flow direction. Accurate simulating results can be gainedwith the grid partition. Inverse flows are captured near the wall at four corners incombustion simulating through the above grid and temperatures simulating agreewell with them measured.

对于大容量四角切圆锅炉的数值模拟,本文给出了一个可以有效减小伪扩散、流体流动方向与网格线方向一致、数目较少、计算精度较高的结构网格,该网格的数值计算结果清晰得捕捉到了四个角向火侧和背火侧靠近壁面处的小回流,而且计算温度与炉膛温度实测值较好的吻合。

Vehicle headlamp provided with a metal halide lamp comprising a discharge vessel surrounded with clearance by an outer envelope and having a ceramic wall which encloses a discharge space containing xenon and an ionizable filling, wherein in said discharge space two electrodes are arranged whose tips have a mutual interspacing EA so as to define a discharge path between them, wherein the discharge vessel has an internal diameter Di at least over the distance EA, and wherein Di is smaller than or equal to 2 mm and the relation EA/Di is smaller than 6, with the special feature that said vehicle headlamp has not more than one band-shaped light-absorbing coating laterally of the discharge path.

一种配置金属卤化物灯的车辆前灯包括:放电容器,其被外壳有间隙地包围,其具有陶瓷壁,所述陶瓷壁封闭包含氙和可电离填充物的放电空间,其中两个电极布置在所述放电空间内,所述电极末端具有相互间隔EA,因而在它们之间定义放电路径,其中放电容器至少沿着距离EA具有内径Di,其中Di小于或等于2mm,以及关系式EA/Di小于6,其特征在于,所述车辆前灯放电路径的侧向方向具有至多一条带状光吸收涂层。

METHODS: Rat bilateral olfactory bulbs and olfactory mucosa at 1/3 nasal septum were obtained, sliced, digested in trypsin, and made into monoplast suspension. At 1×109/L, cells were incubated in uncoated 25 cm2 culture flask. At 18-20 hours, cell suspension was moved into another uncoated 25 cm2 culture flask (the first differential adhesion). At 24 hours, cell suspension was moved into a poly-D-lysine-coated 25 cm2 culture flask or poly-D-lysine-coated 6-well culture plate (the second differential adhesion). At 48 hours, parenchyma cells were removed after a half of medium was changed.

完整取大鼠双侧嗅球及剪取鼻中隔后1/3嗅黏膜,剪碎后胰酶消化,制成单细胞悬液,按1×109 L-1密度接种于未包被的25 cm2玻璃培养瓶中,18~20 h后将细胞悬液转移至另一未包被的25 cm 2玻璃培养瓶中(第1次差速贴壁),24 h后再将细胞悬液转移至经多聚右旋赖氨酸包被的25 cm2塑料培养瓶或经多聚右旋赖氨酸包被的6孔培养板中(第2次差速贴壁),接种培养48 h后半量换液以去除杂细胞。

Both the two sides superior thyroid arteries were mainly arose from antero-endhymenine wall of genesis arteries (58.45%), inferior thyroid arteries of the thyrocervical trunk from endhymenine wall, and the ima thyroid arteries of genesis arteries all from anterior wall.

甲状腺上动脉源自起源动脉前内壁最多(58.45%);起源于甲状颈干的双侧甲状腺下动脉均源自其内侧壁;甲状腺最下动脉均源自起源动脉前壁。

Electromyography of the anterior abdominal wall, right chest wall and the anterior scaleni, intraabdominal pressure, intrathoracic pressure, respiratory rate, pelvic floor kinematics, and downward leg pressure of the nonlifted leg were compared between a left and right ASLR.

对比了双侧主动直腿抬高时的前腹壁和右胸壁肌电图、腹内压、胸内压、呼吸频率、骨盆底运动和对侧下肢下压力。

The results show that in the formation process of tumble during the intake stroke,two vortexes are generated in the cylinder.

增加流经排气门侧的进气气流或抑制直接沿进气门侧缸壁流入气缸的气流,都能提高滚流强度。

The posterior wall does not proliferate and remains as the poorly defined pars intermedia.

以后其前壁发育成垂体的远侧部,后壁将不会增殖而形成垂体的中间部。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?