依次的

Methods The total RNA was extracted from the brain of human embryo. The segment of open read frame of DOC-1 was amplified by RT-PCR and was further cloned into the vector, pMD18-T, and the prokaryotic expression vector, pGEX-4T-1, by turns, to produce the new construct pGEX-4T-1-DOC-1. After restriction enzymes digestion analysis and sequenced, pGEX-4T-1-DOC-1 was transformed into E.coli BL21（DE3）. GST-p12 recombinant protein was expressed under IPTG induction and purified by affinity column.

从人胎脑组织中提取总RNA，经逆转录-聚合酶链式反应扩增DOC-1的CDS序列，再通过基因重组技术将该基因片段依次克隆到pMD18-T和pGEX-4T-1载体中，构建融合表达载体pGEX-4T-1-DOC-1，经酶切、测序鉴定后，用该重组质粒转化E.coliBL21，用IPTG诱导表达，Glutathione Sepharose 4B柱亲和层析纯化重组蛋白。

Before the rank 8 country is ordinal it is the United States, England, China, France, Canada, Germany, Russia, Australia.

排名前8位的国家依次是美国、英国、中国、法国、加拿大、德国、俄罗斯、澳大利亚。

The net is bought trade amount platoon is advanced 3 area is ordinal for Shanghai, Guangdong and Zhejiang.

网购交易总额排在前三名的地区依次为上海、广东和浙江。

This paper points out that the ore-forming pattern of this mineral deposit is ore-bearing basic-superbasic magma melting-crystalline different and ore magma impulse injection in turn. It indicates there maybe has a ore shoot in the bottom of III rock body(interrupted below 650m), and advises to geophysical prospecting in the mining shaft.

并指出本矿床为含矿基性-超基性岩浆熔离-结晶分异和矿浆依次脉冲式贯入的铜镍硫化物矿床成矿模式，指明Ⅲ号岩体下部(650m以下中断)可能存在一富矿体，并建议进一步做井中物探证实。

The statistical results of ultra-high pressure metamorphic rocks' logging susceptibility indicated that the susceptibility became low gradually from serpentinite to quartz eclogite,rutile-bearing eclogite,orthogneiss,retrograded eclogite,phengite eclogite,paragneiss,chlorite amphiboli...

测井磁化率统计结果表明CCSD主孔100~2 000 m超高压变质岩的磁化率从高至低依次为：蛇纹岩、石英榴辉岩、金红石榴辉岩、正片麻岩、退变质榴辉岩、多硅白云母榴辉岩、副片麻岩、绿泥石角闪岩、角闪岩。

The result of orthogonal experiment showed that the sequence of the factors affecting extraction ratio of the LPC was flocculation method, solid-liquid ratio and the amount of adding salt. The optimal combination condition was solid-liquid ratio 1:7, salt concentration 0.3% and ethanol precipitation method.

对固液比、盐浓度、凝絮方法3因素3水平正交试验结果表明，影响聚合草叶蛋白提取率的因素依次为凝絮方法＞固液比＞加盐量，最佳条件组合为固液比1：7、盐浓度0.3%、乙醇法沉淀。

They in turn revised its orthography and added a number of written vowels.

他们又依次地修改了一些拼写，加上了许多手写的元音。

The results showed that Osmoregulation of water stress are high than normal water at the same time.

渗透调节物质含量在12月和1月最高。运用水势、可溶性糖含量和K+含量评价三个树种对干旱和低温适应强弱的顺序依次为侧柏，女贞和小蜡。

Combined bed is refers to the water in turn pass the system of the cation exchanger of hydrogen modeland the anion exchanger of oxyhydrogen model,by the combined bed can basically remove various mineral salt of the water .

複床是指水依次通过装有氢型氧交换器和装有氢氧型阴交换器的系统，通过复床可将水中各种矿物盐基本除去。

Of the research group commited a crime under the direct control of psychiatric symptoms, the commonest psychiatric symptoms were in order delusion of persecution, auditory hallucination, delusion of reference and paralogia.

研究组89%在精神症状直接支配下作案，最常见的精神症状依次为被害妄想、幻听、关系妄想、逻辑障碍。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。