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Successor Erdeni France King (note: the temple Chair) followed by JI Xue Baluosangwei color, Chawaluosang Gyaltsen, white Li Danda, E Baluo Cinque beads, mother hot song rolling, salad stack Zhu Jia Luosang down white.
继任额尔德尼法王者依次是吉学巴洛桑维色、察瓦洛桑坚赞、白利丹达、厄巴洛桑克珠、娘热曲扎、色拉堆竹加洛桑降白。
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Sample 2 had low toxicity to Daphnia magma and Brachydaniorerio, and its Tua values were 4.39 and 1.97, respectively.
水样2对大型蚤和斑马鱼都属于低毒等级,其Tua依次分别为4.39和1.97。
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The RT-PCR product was inserted into pTG19-T vector and transformed into E. coli successfully. By blastn, the sequence results of Kunming mus musculus were in complete accordance with the conservative sequence of Genbank NR_003278 (791bp-1153bp). By Blastn in NCBI, the sequence with little difference among animals was confirmed to be conservative. After Blastn, fourteen complete CDS coding for different animals were chosen. According to VECTOR NIT 9.0 software, the similarities between Kunming mus musculus and bos taurus, homo sapiens, erinaceus europaeus, cricetulus griseus, sus scrofa, dasypus novemcinctus, rattus norvegicus, rabbit, equus caballus, macaca fascicularis, didelphis virginiana, monodelphis domestica and vombatus ursinus was 67%, 100%, 100%, 36%, 100%, 100%, 67%, 100%, 100%, 92%, 99%, 99% and 99%. In the phylogenetic tress constructed with the forteen 18S rRNA by Treeview, the Kunming mus musculus clustered with cricetulus griseus, sus scrofa and rabbit, which was nearer to cricetulus griseus and was most far away from macaca fascicularis.(3) After sencodary structure analyses of 18S rRNA of mus musculus, an oligonucleotide fragment for RNAi was designed and synthesized, which was transformed into plasmid, and restriction enzyme analyses and sequencing results should the expression plasmid pGPH1/ GFP/Neo-mouse-sh 18S rRNA were constructed for RNAi successfully.
结果①通过RT-PCR检测显示18S rRNA基因在小鼠卵巢组织和单个GV期、MⅠ期卵母细胞中均有表达,且在未成熟卵母细胞中,MⅠ期的表达明显强于GV期的表达;②RT-PCR产物克隆测序结果显示:昆明小鼠18S rRNA基因保守区序列与基因库序列[NR_003278保守区部分(791bp~1153bp)]完全一致;Blastn比对结果发现:在不同物种中差异较小,选出14种生物18S rRNA全序列经VECTOR NIT 9.0软件分析,提示昆明小鼠18SrRNA与牛、人类、刺猬、中国仓鼠、猪、犰狳、褐鼠、兔子、马、食蟹猴、负鼠、短尾猊、袋熊的18S rRNA的相似率依次为67%,100%,100%,36%,100%,100%,67%,100%,100%,92%,99%,99%,99%;Clustal 1.81和Treeview构建出的分子进化树表明:在上述14种生物中昆明小鼠与中国仓鼠进化关系最近,与兔子、猪聚成一簇,与食蟹猴进化关系最远;③根据18S rRNA二级结构设计并合成RNA干扰寡核苷酸片段,重组质粒经过限制性内切酶及测序表明成功构建了pGPH1/GFP/Neo-mouse-sh 18S rRNA干扰表达质粒。
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The as-grown sapphire boule (φ110×80mm3) turns out colorless and transparent after being decolourized and decarbonized in air and hydrogen successively at high temperature.
将TGT法生长的Al2O3晶体(φ110×80mm3)依次经过高温氧化气氛、高温还原气氛脱碳、去色退火实验,即&两步法&退火实验,晶体变成无色、透明。
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Person the action in achieving goal of public security higher education is ordinal and degressive.
三者在实现公安高等教育目标中的功能依次递减。
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The manner of death was deduced comprehensively based on all above findings. Results: There were 85 cases determined as drug abuse death in Shaanxi area from Oct. 1995 to Oct. 2001, including 63 males and 22 females. 70% of the subjects detected in postmortem biological samples were heroin /morphine. Hyperaemia, edema, hemorrhage and arteriolar sclerosis of multi-organs; cells denature and necrosis; foreign body in pulmonary, as well as needle puncture tracks on skin and dermapostasis, were common pathological observations. Incidences of tuberculosis(21%),cirrhosis(12%) and hepatitis(9%) were higher than those found in general autopsy population. No AIDS case was found.
结果··:陕西地区1995年-2001年有85例经法医鉴定为药物滥用死亡,其中男63例,女22例;尸体血液、脏器、毛发内检出的滥用药物70 %是海洛因/吗啡;多药混合滥用特点明显;最常见的病理变化包括:多脏器淤血、水肿、出血、小动脉硬化,细胞变性、坏死,肺内异物及皮肤注射针眼、脓肿等;常见合并的传染性疾病依次为:结核病(21 %)、肝硬化(12 %)和病毒性肝炎(9 %)等,AIDS未检出。
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The preparation method comprises the following steps in sequence:(1) metric acetonitrile is added into a dual-bottleneck distilling flask that is provided with a refluxing and condensing tube, and simultaneously, long-chain dialkyl methyl tertiary amine and relevant saturated dihalide are added thereof;(2) nitrogen is pumped for 10 to 20 minutes for deoxidizing;(3) a magnetic mixing reaction is carried through for 10 to 15 hours under the condition of 78 to 83 DEG C.
其制备方法依次包括以下步骤:(1)在装有回流冷凝管的二颈蒸流烧瓶中,加入计量的乙腈,同时按一定比例加入二长链烷基甲基叔胺与相应的二卤代烷;(2)通氮气10-20分钟,排除氧气;(3)在78-83℃条件下磁力搅拌反应10~15h。
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This experiment adopted organic solvent extraction step by step,following deproteinization,dialyzation,alcohol-precipitation and layer-separation of dextran,to reveal the unknown effective ingredients in pumpkin,which have influence to blood sugar in normal mice or model mice of diabetes mellitus.
为探寻南瓜中对正常及糖尿病模型小鼠血糖有影响的未知有效成分,本实验依次采用有机溶剂分步萃取、除蛋白、透析、乙醇沉淀及葡聚糖柱层析等分离手段,以小白鼠血糖值作为筛选活性成分的指标,在分离的每一阶段对分离所得各个组分进行活性定量评估,并追踪活性最强的部分,最后分离纯化出降血糖活性最强的组分G———南瓜多糖。
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Results When cellulase and pectinase compound,amlyse and diastatic enzyme were added in order in the method of stepwise biocatalysis,98% diosgenin from plants was extracted.IR spectrum of the product was in accordance with that of reference substance and the purity of it is more than 95% according to its HPLC chromatogram.
结果利用阶梯生物催化法,当纤维素酶和果胶酶复合酶制剂、淀粉酶以及糖化酶按顺序依次加入时,能够将植物中98%的薯蓣皂苷元提取出来,其产品的红外谱图与对照品谱图相吻合,经HPLC谱测定薯蓣皂苷元质量分数95%以上。
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Results: Recoveries over 90%,the relative standard deviation of sample (n=6) was 1.8% for dichloroacetic acid and 1.9% for trichloroacetic acid and the detection limit was 0.83 μg/L and 0.4 μg/L respectively.
结果:本法的加标回收率在90%以上,6份平行样品测定的相对标准偏差二氯乙酸为1.8%,三氯乙酸为1.9%,检出限依次为0.83μg/L和0.4μg/L。
- 推荐网络例句
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I didn't watch TV last night, because it .
昨晚我没有看电视,因为电视机坏了。
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Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.
今年以来,在北京的很多小区里,电梯液晶电视被撤了下来。
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I'm running my simile to an extreme.
我比喻得过头了。