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The mRNA of pituitary glands from seven important economic Pleuronectiformes fishes was extracted,the cDNA sequences of GH gene were then isolated from Cleisthenes herzensteini,Limanda yokohamae,Pleuronichthys Cornutus,Cynoglossus robustus,Platichthys bicoloratus, Zebrias zebra and Paralichthys dentatus with RT-PCR method by using designed specific primers based on the reported GH cDNA sequences of Pleuronectiformes. The results of sequencing showed that lengths of the above cDNAs are as follows: 479 bp,564 bp,519 bp,440 bp,564 bp,440 bp and 522 bp.The GH cDNA sequences encode mature polypeptide of about 140 to 170 amino acid.The full-length growth hormone cDNAs of Paralichthys lethostigma and Scophthalmus maximus were cloned by Switching Mechanism At 5\' end of the RNA Transcript RACE technology.
从7种重要鲽形目经济鱼类——高眼鲽、黄盖鲽、木叶鲽、宽体舌鳎、石鲽和条鳎、夏鲆的脑垂体中提取mRNA,并采用RT-PCR方法,根据已克隆得到的鲆鲽鱼类生长激素基因的保守性序列设计特异性引物,克隆了含开放阅读框的生长激素(Growth Hormone,GH)cDNA序列,测序结果显示,高眼鲽、黄盖鲽、木叶鲽、宽体舌鳎、石鲽、条鳎和夏鲆的GH cDNA长度依次为479 bp、564 bp、519 bp、440 bp、564 bp、440 bp和522 bp,编码140-170个氨基酸的成熟GH多肽片段;从漠斑牙鲆和大菱鲆脑垂体中提取mRNA,利用SMART-RACE技术建立脑垂体cDNA文库,并从该文库中克隆出大菱鲆和漠斑牙鲆生长激素基因cDNA全长序列。
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The results show that the mineral admixtures can reduce C3A quantity in the cement material, and the pozzolanic reaction can consume lots of hydration product-Ca2, Which makes specimens close-grained, has obvious prevention function towards the sulfate corrosion. The anti-sulfate corrosion effects of various mineral admixtures from finer to poorer in turn are as follows: 30% fly ash, 30% fly ash + 30% slag, 60% slag, 30% steel slag + 30% slag, 30% steel slag, 30% slag, the blank type.
研究结果表明:加入矿物掺合料降低了试件的胶凝材料中的C3A含量,其火山灰反应消耗了大量的水化产物Ca2,且提高了试件的密实度,对硫酸盐侵蚀具有明显的预防缓解作用;各矿物掺合料的抗硫酸盐侵蚀效果由优到劣依次为:30%粉煤灰,30%粉煤灰+30%矿粉,60%矿粉,30%钢渣+30%矿粉,30%钢渣>30%矿粉,空白样。
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Feedback loop----A closed path that connects an action to effect on the surrounding conditions which, in turn, can influence further action.
反馈循环——在一个循环条件下连接行为和结果的封闭回路,依次的,能影响更多行为。
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Finally, the competition advantage status of Jingchuan Hotel, the first themed hotel qualified by CNTA, is analyzed, according to the competition advantage formation model of themed hotel. And then, three strategic suggestions are put forward. The order of more emergent suggestion is building up the Jingchuan Hotel's spirit referring to the theme, cultivating the talents in the theme, enhancing the hardware facilities related to the theme.
最后,依据本研究中形成的主题饭店竞争优势形成模型,对成都京川宾馆——中国第一家在国家授牌和证书中,明确写有"文化主题"字样的主题饭店——的竞争优势现状进行了分析,并针对京川宾馆发展的轻重缓急,提出了三个战略性的建议,由急到缓依次是:锻造饭店的主题精神、培育饭店的主题人才、夯实饭店的主题硬件。
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The agents of legal change, Fictions, in turn to bear on the Equity, and Legislation, are brought primeval institutions, and at every point of the progress, a greater number of personal rights and a larger amount of property are removed from the domestic forum to the cognisance of the public tribunals.
改变法律的媒介即拟制、衡平和立法,依次在原始制度中发生作用,而在每一个发展过程中必有大量的个人权利和大量的财产从家庭审判庭中移转到公共法庭的管辖权之内。
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Results Among the familiar diseases in females,colpitis,cervicitis,hyperplasia of mam-mary glands,adnexitis and uterine leimyoma were the first five diseases with most cases.
结果 当前妇女常见病、多发病排前5位的依次为阴道炎、宫颈炎、乳腺增生、附件炎、子宫肌瘤。
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When the command interpreter (the "shell") runs a program, three files are open, with file descriptors 0, 1, and 2, called the standard input, the standard output, and the standard error.
当命令解释程序运行一个程序的时候,它将打开3个文件,对应的文件描述符分别为0、1、2,依次表示标准输入、标准输出和标准错误。
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Results the overall injury prevalence was 4.58 ‰,and in details,the prevalence gender ratio(boys vs. girls) was 3.48. further analysis showed that the injury prevalence among adolescents was associated with the school types. the campus was the main place of injury occurrence (82.8%). incaution was the leading cause for adolescent injuries accounting for 85.5%. the leading four types of injuries were collision (56.9%),falls (28.8%),knife-cutting or sharp weapon hurt (4.7%) and traffic accidents (1.8%). the most common types of injury were fraction (41.6%) and trauma (36.4%).
结果 学生伤害的平均年发生率为4.58‰,男、女生伤害发生率比值为3.48;伤害发生率与校别有较密切的关系;学校是学生伤害发生的最主要场所,占82.8%;不慎是造成学生伤害发生的最主要原因,占伤害发生原因的85.5%;排在前4位的伤害类型依次是碰撞(56.9%)、跌落(28.8%)、切割穿刺(4.7%)、交通事故(1.8%);伤害性质主要以骨折和外伤为主,分别占伤害数的41.6%和36.4%。
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The result expressed:(1)the approbation degree of the sports values factor which influenced the university student in order is: personal sport ability, interests in the sport, family environment, p.e teacher ,compeer community, campus culture, society and sports teaching (2)the analysis of diverse returning indicated: interests in the sport, compeer community, campus culture, society and sports teaching, those are the main factor that influences sports values and attain the remarkable level, but the factors of personal sport ability, family environment, p.e teacher didn't attain the notable level.
结果表明:(1)大学生体育价值观影响因素的认同程度依次为:个人运动能力、体育兴趣、家庭环境、体育教师、同辈群体、校园文化、社会和体育教学;(2)多元回归分析表明:体育兴趣、同辈群体、校园文化、社会和体育教学5个因素是影响体育价值观的主要因素且达到显著水平,而个人运动能力、家庭环境和体育教师因素没有达到显著水平。
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This article takes the philology angle of view, ordinally sawing about the issues as compilatory origin, complier, explanatory notes, stuff chosen, classic items quote, try to analysis the compilation function and scholarly tenet of the Emperor Kang Xi's Scriptures roundly.
本文从文献学的角度入手,以问题为序,依次就御纂诸经的修纂动机、纂修人、凡例、取材问题、学术宗旨等问题,加以比较全面的分析和考察。
- 推荐网络例句
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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.
每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。
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Maybe they'll disappear into a pothole.
也许他们将在壶穴里消失
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But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.
但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。