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A pair of primers was designed according to the coat protein gene of Arabis mosaic viru and used to amplify a 990 bp partial gene of the cp by reverse transcription-polymerase chain reaction, the 990 bp partial gene was cloned into the pEGX-9p-1, a prokaryotic expression vector fused with GST. The recombinant vector was transformed into E. coli BL21. The fusion protein was successfully expressed in E. coli BL21 induced with the IPTG at 37℃.

依据报道的ArMV外壳蛋白基因(coat Protein, Cp)序列设计引物,通过RT-PCR 扩增得到长约990bp的ArMV cp基因亲水基团部分片段,将目的片断克隆到原核表达载体pEGX-6p-1中,构建ArMV cp基因与GST蛋白融合表达载体pEGX-cp,重组载体化大肠杆菌BL21,经IPITG诱导后,融合蛋白GST-cp得到了特异表达。

These studies could provide the basic data and research achievement on analysis the difference of plant transpiration and water consumption between different tree species. It contribute to instructional significance to regulate and control the moisture relations between every factor, and solve contradiction between moisture providence and demanding. At the same time, It will be provided the theoretical evidence for plants choosing and arranging ,and properly using water resources in arboriculture and maintenance.

这些研究为分析造成不同树种之间蒸腾耗水的差异提供了基础资料,对于调控各因素之间的水分关系,解决水分供需关系矛盾有重要的指导意义,同时也为植物选择与配植,栽培与养护中合理地利用水资源提供了理论依据

The curve provides the basis for the testing of stress intensity factor with crack tip arc the strength analysis of component with arc crack

该曲线为带圆弧裂纹的应力强度因子测试和带圆弧裂纹构件强度计算提供了依据

Based on the analysis of three-dimensional power conductor for DC arc furnace, the electric arc deflection model was set up and the control system of the arc direction was configured. According to the bus bar distribution at the bottom electrodes cooled by water, the arc direction control principle and its configuration were described, The simulation results show that the control system can restrain the electric arc deflection and control the arc direction.

在分析直流电弧炉三维导体布置的基础上,建立了直流电弧的偏工以此作为电弧位置控制的依据,针对水冷钢棒式底电极型的直流电弧炉,阐述了其电弧位置控制的原理和系统组成,并进行了仿真研究,结果表明控制系统可以有效地纠正电弧的偏向总是并可控设定点自由控制电弧以位置。

All these results lay a foundation for the improvement of arc welding technology and arc waveform control.

分析结果可为改进焊接工艺和波形控制方法提供依据

Caculation of principle error of involute profile processed by Archimedean hobbing cutter ;2. The equation of single hobbing cutter 's locomotion locus and the mathematical model of its instantaneous cutting area are established.

建立了单把滚刀旋切运动的轨迹方程和瞬时切削面积的数学模型,在此基础上对旋切面积的数学模型进行分析及简化,为旋切技术的实际应用提供了有力的依据

A formula was deuced, which shows the relation between the outlet angle of center line of channel and wrap angle, variant coefficient, if variant Archimedes spiral is assumed the curve of center line of plate plane. It is the basis for controlling outlet angle of channel.

推导了采用变异的阿基米德螺线作为平面流道中线时,中线出口角与包角和变异系数之间的关系式,为设计中控制流道出口角提供了依据

On the basis of the analysis of the scores, the author makes conclusions about the characteristics of Schubert"s last three piano sonatas as followings: 1 The device of universal development in the movements composed in sonata form and rondo-sonata form is the most distinctive feature in the last three sonatas, which is also decisive in the trend of the whole movement and makes the developments relatively "still". 2 In the device of themes, the method of irregularity is preferred. However, By using extension, abbreviation, repetition and further repetition of periods, Schubert brings those themes to ingenious balance in both architectonics and aesthetics. 3 Chain method is applied in the connection of themes, in which the previous material is taken as basis and then modified together with the variations of new materials. By this way, the theme is continuously developed. 4 The principle of the dominant-subordinate is followed in Schubert"s three-key exposition, and the process of each key is extended.

本文在详尽分析乐谱的基础上,对最后三首奏鸣曲的主要创作特点进行了以下总结: 1、奏鸣曲式和奏鸣回旋曲式乐章中展开的普遍化是最后三首奏鸣曲最为显著的特点,它决定了整个乐章的走向,并使展开部具有相对"静止"的特点; 2、舒伯特的主题写作中更喜欢运用非方整性写法,并通过自身材料的延长、缩减和重复或者更高一级的段落重复使其达到了结构上和审美上的巧妙平衡; 3、舒伯特喜欢用链式的主题贯穿方式,音乐以之前的材料为依据,经过变化后再加入新的材料,用这种方式不间断地发展; 4、舒伯特的三调呈示部遵循从主到属的总体原则,并扩展了调性历程。

This paper views consideration in perspective of the characteristics and practice of archival value.

依据档案价值的特性及档案价值实现的规律,对档案价值与档案价值的实现进行了思考。

By using solidworks software,The models of AOD converter shell and molten steel for obtaining the areal coordinates are created to make refined calculation about the tilting moment s of the new or old converters.

运用solidworks软件建立AOD炉及钢液的三维实体模型,以获取其重心坐标,并对新炉、旧炉倾动力矩进行了精确的计算,依据倾动力矩变化曲线提出了防止炉体自动倾翻的耳轴中心安全高度的合理位置。

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推荐网络例句

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车,和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特,那个是爱朗。

Articulate the aims, objectives and key aspects of a strategic business plan.

明确的宗旨,目标和重点战略业务计划。