使过量

Inaddition,we extract total RNA from cultural U251 cell of humanglioma,make coding gene extron amplification of TfR through ReverseTranscription-Polymerase Chain Reaction,construct expressionplasmid of pCDNA3.1-TfR after digestion of enzyme andconjunction,do transfection to U251 cell by liposome afteridentification,cause excess expression,detect the efficiency of transientexpression of TfR protein by pEGFPN1-TfR, sieve positive cell ofpCDNA3.1-TfR through G418, choose positive cell clone, detect theexpression of TfR in anteroposterior period through Western blot,FCM,cell immunochemistry and mRNA through RT-PCR, identify the effect of transfection.

另外，培养人脑胶质瘤U251细胞，进行细胞总RNA抽提，运用RT-PCR方法进行TfR基因的完整编码区外显子的扩增，经过酶切、连接后构建pCDNA3.1-TfR的表达质粒，鉴定正确后按脂质体转染方法转染人胶质瘤U251细胞，使其过量表达，通过pEGFPN1-TfR检测其瞬时转染的效率；用G418对稳定表达pCDNA3.1-TfR的细胞进行筛选，挑选阳性细胞克隆，运用Western blot、流式细胞检测技术和细胞免疫化学以及mRNA半定量的方法检测TfR在转染前后的表达水平，鉴定转染的效果。

With the increase of population and development of modern industry and agriculture in the last several decades, the aging process of lakes increased by human unwarrantable activities, causing supernutrition in the waterbody and greatly changed the water quality.

近十几年来，随着人口的增长，现代工业和农业的发展，湖泊的老化过程受到人类活动的影响而大大加速。过量营养物质的富集，使水体产生了一系列的变化。

To understand whether the GST can protect plant from the damage of ultra-violet radiation,a UV inducible GST gene was isolated from an Arabidopsis cDNA library.The plant expression vector containing the GST cDNA was constructed and transferred into Arabidopsis thaliana plants by the Agrobacterium-mediated vacuum method.Molecular genetic analyses showed that the overexpression of the UV inducible GST could increase the tolerance of the transgenic plants to UV radiation

为了阐明GST在紫外辐射下是否对植物有保护作用，以紫外强烈诱导表达的GST cDNA为探针，筛选拟南芥cDNA文库，获得了这种GST的全长cDNA；利用此cDNA构建植物表达载体，并通过农杆菌介导法转化拟南芥，使其在拟南芥中得到大量表达；通过对转基因植株的紫外辐射耐性分析，证实了该GST的过量表达可明显增强拟南芥对紫外辐射损伤作用的耐受性。

An acid/base indicator is a weak organic acid or a weak organic base whose undissociated from differs in color form its conjugate base or its conjugate acid form.

酸碱指示剂一般是弱的有机酸或有机碱，其中酸式及其共轭碱式具有不同的颜色，当滴定至等当点时，过量的酸或碱使指示剂得到或失去质子，由碱式变为酸式，引起颜色的变化。

In excess it will make the wine smell and taste vinegary.

如果过量，就会使酒尝起来有股酸味。

Witch hazel can be helpful to control excessive perspiration and also used in a final hair rinse helps give the hair a shine and lustre.

可以控制过量的排汗。也可以用于最后的头发漂洗，使发质闪亮有光泽。

On the basis of regeneration system of cotyledonary nodes, using Agrobacterium tumefaciens, the Mn-SOD gene was introduced into cucumber plants to express excessively, to improve ability of cleaning ROS and stress tolerance on environmental stress. The new Mn-SOD transgenic materials could be used for study of resistance physiology.

本研究在优化黄瓜再生体系的基础上，通过根癌农杆菌介导法，将Mn-SOD基因转入黄瓜植株，使SOD在植株体内过量表达，提高其在环境胁迫下清除活性氧的能力，从而提高黄瓜植株的抗逆性，并为抗性生理的研究提供有价值的材料。

And to make the product be easy of purification, the excess of amine was reduced to 50% from 150%. Then the diiodoalkane reacted with dialkylmethylamine into diquaternary ammonium iodide, and obtained three diquaternary compounds whose spacer groups were -(CH2)6-, N,N"-dimethyl- N,N,N",N"-tetraoctyl-1,6-hexane diammonium diiodide, N,N"-dimethyl-N,N,N",N"-tetradecyl-1,6-hexane diammonium diiodide, N,N"-dimethyl-N,N,N",N-tetradodecyl -1,6-hexane diammonium diiodide.

对于更长间隔链的双子型季铵盐，一方面为了加快亲核取代反应速率，先将1，6-二氯己烷、3-氧杂-1，5-二氯戊烷转化成1，6-二碘己烷、3-氧杂-1，5-二碘戊烷，另一方面为了降低产物提纯的难度，将叔胺的过量由150%降到50%，使二碘代烷与双长烷基甲基叔胺在相同的温度下、反应相同的时间，经过相同的后处理，得到3个间隔链为-(CH2)6-的双子型季铵碘化物，(C8H17)2N+(CH3)(CH2)6 (CH3)N+(C8H17)2·2I-、(C10H21)2N+(CH3)(CH2)6(CH3)N+(C10H21)2·2I-和(C12H25)2N+(CH3)(CH2)6(CH3)N+(C12H25)2·2I-。

ASR1 is a gene from early auxin-responsive GH3 gene family, and the protein encoded by this gene is overexpressed in the mutant, which results in the following morphological phenotypes including dwarf, epinastic rosette leaves, and restrained lateral root formation, etc. Meanwhile, ASR1 may modify the plant defense signaling molecule salicylic acid in vivo, and therefore asr1 mutant is more susceptible to the infection from the pathogen Pseudomonas syringae.

ASR1属于生长素相关的GH3基因家族，它编码的蛋白质在突变体内过量表达，并造成植株矮化，莲座叶片向下卷曲，侧根发生受到抑制等形态表型的改变，同时，ASR1可能还参与了植物体对抗病信号分子水杨酸的调控或修饰过程，并使asr1突变体对病原菌丁香假单胞杆菌的侵染更敏感。

When the body is hungry, Gambogic fruit extract of the major components of HCA can be converted into glycogen energy to meet the physical requirements to improve the excessive eating habits, so that the body completely lazy stubborn fat burning, but also to prevent the accumulation of fat, no fat rebound The trouble, to achieve a comprehensive and healthy slimming effect.

当身体感到饥饿时，藤黄果提取物的主要成分HCA能将肝糖转化成能量，应付身体所需，改善过量饮食习惯，彻底使体内懒惰顽固脂肪燃烧，更可预防脂肪积聚，没有脂肪反弹之烦恼，达至全面而健康的纤体效果。

The absorption and distribution of chromium were studied in ryeusing nutrient culture technique and pot experiment.

采用不同浓度K2CrO4(0，0.4，0.8和1.2 mmol/L)的Hoagland营养液处理黑麦幼苗，测定铬在黑麦体内的亚细胞分布、铬化学形态及不同部位的积累。

By analyzing theory foundation of mathematical morphology in the digital image processing, researching morphology arithmetic of the binary Image, discussing two basic forms for the least structure element: dilation and erosion.

通过分析数学形态学在图像中的理论基础，研究二值图像的形态分析算法，探讨最小结构元素的两种基本形态：膨胀和腐蚀；分析了数学形态学复杂算法的基本原理，把数学形态学的部分并行处理理念引入到家实际应用中。