使迁移

Epidermal cells near the wound dedifferentiate and proliferate forming blastema and/or migrate to the wound plane forming a pre-epidermis consisting of several layers of stem cells covering the wound plane, which then re-differentiates further forming fully developed epidermis. Cells in the parietal peritoneum are also induced by injury to dedifferentiate into stem cells which then proliferate and migrate along the parietal peritoneum to the wound place forming a pre-peritoneum which re-differentiates into fully developed parietal peritoneum.

伤口愈合包括伤口闭合、顶端表皮层及体腔上皮的新生：创伤后的前4 d，残腕顶端的肌肉组织向伤口处迁移并重排使伤口闭合；创伤附近的表皮层细胞发生脱分化并增殖后迁移到创伤面形成由数层干细胞组成的前表皮层，之后进一步分化形成表皮层；而体腔上皮细胞在创伤诱导下也脱分化并增殖，然后沿体腔上皮迁移到创伤处形成"前体腔上皮"，经再分化形成新的体腔上皮。

In order to complete the investigation, impersonality feasible experiment solutions and devices are designed independently to systemically investigate on activation and transfer characteristic of soil heavy metal under acid rain effect. Primary results are shown as followings:1. PH measuring results of soil solution and eluviation solution show that the ability of experimental plot the acid buffer capacity of soil is stronger under the experiment condition,there is no obvious acidification in soil which after acid rain eluviation.The result has substantial differences with that gotten by most of investigationers in similar work before.

这一结果与以往类似研究中多数研究者得到的结论存在本质区别，原因可能在于此次试验的试验条件与以往研究的试验条件不同，其中最主要一点是在本次试验中使用的土壤样品的重量比以往试验均显著增大，而且使用的是土壤结构和组成等均未经破坏的原位土，从而使入渗酸雨与承受酸雨沉降的土壤之间的比例关系更接近自然酸雨沉降过程，以此为基础得到的试验结果应该更加客观、可靠。2、酸雨对盐基离子活化迁移产生的影响勿庸置疑，但是，在盐基离子活化迁移的过程中，并没有出现预想中的重金属元素活化迁移现象，表明土壤中的重金属元素并不如理论分析的那样对酸雨沉降反应敏感。

Hydrothermal treatment and pH value swing could shift the micropore distribution of alumina to macroporous direction, and decreased surface areas; the surfactants addition could shift the micropore distribution of alumina to microporous direction, and increase surface areas.

其中pH值交替变动和水热处理使氧化铝微孔分布向大孔方向迁移，比表面降低；加入表面活性剂使氧化铝微孔分布向小孔方向迁移，比表面增加，制备出了低比表面≤150平方公尺g^(-1和高比表面≥250平方公尺g^(-1的大孔容氧化铝载体。

The study of vertical channel with high field effect mobility by Ni induced lateral crystallization, which can form larger grains on channel regions and reduce grain boundary defects. We detect the method of Ni induced lateral crystallization through high field effective mobility, but that accumulates too much Ni on floating N+ region and causes defects and metal contamination. As Ni is used to form silicde and then proceed to low temperature annealing, we can observe the floating region that has no Ni acumination regions or contaminations. This aids us in obtaining good electronic performance of this device, not only the remote field effective mobility but also the vast improvement of sub-threshold swing and on/off currents.

接著，是延续垂直通道的研究，希望能有效的提升电子迁移率，因此以镍金属诱导横向结晶的方式使通道形成较大的结晶，减少矽晶粒之间的缺陷，而在初步的研究，发现以镍金属直接诱导横向结晶的方式，经由低温长时间回火之后，由於镍金属扩散的关系，完成诱导结晶之后，有过多的镍金属聚集在顶部参杂的中间区域，而造成过多的缺陷累积，虽然电子迁移率有效的提升，但整体的电性并不如预期，因此提出先形成镍金属矽化物后，将未反应的镍金属或金属氧化物去除之后，再进行镍金属矽化物诱导横向结晶处理，经由结果观察得知在顶部掺杂区域部分没有明显的镍金属累积或污染，并且得到很好的整体电性，除了电子迁移率有效提升之外，次临界特性以及导通电流都能有效改善。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

Epidermal cells near the wound dedifferentiate and proliferate forming blastema and/or migrate to the wound plane forming a pre-epidermis consisting of several layers of stem cells covering the wound plane, which then re-differentiates further forming fully developed epidermis. Cells in the parietal peritoneum are also induced by injury to dedifferentiate into stem cells which then proliferate and migrate along the parietal peritoneum to the wound place forming a pre-peritoneum which re-differentiates into fully developed parietal peritoneum.

伤口愈合包括伤口闭合、顶端表皮层及体腔上皮的新生：创伤后的前4 d，残腕顶端的肌肉组织向伤口处迁移并重排使伤口闭合；创伤附近的表皮层细胞发生脱分化并增殖后迁移到创伤面形成由数层干细胞组成的前表皮层，之后进一步分化形成表皮层；而体腔上皮细胞在创伤诱导下也脱分化并增殖，然后沿体腔上皮迁移到创伤处形成&前体腔上皮&，经再分化形成新的体腔上皮。

The ionic conductivity of polymer electrolytes equaled 3.56×10-4 S·cm-1 when the mass fraction of silicotungstate lithium was 8.5% in polymer film. The lithium ions transference number, which was measured by the method of combination of AC impedance with DC polarization, decreased with increase of the mass fraction of Li4SiW12O40 in the porous polymer film.

为了提高聚合物电解质的Li+离子迁移数，人们设法增大阴离子的体积[6~8]使其难以迁移而达到提高Li+离子迁移数的目的，很少应用含有多个Li+离子的锂盐做聚合物电解质的Li+离子载体。

Following chemical activation, blastocysts rate of the treated oocytes was similar to untreated oocytes.8 Following fertilization, however, few oocytes inhibited with CHX developed into morulae/blastocysts, due to a high incidence of polyspermy.9 Cortical granule migration occurred during inhibition, but CHX inhibition impaired CG migration, significantly no oocytes inhibited by CHX completed CG migration after maturation.10 CHX inhibition had no effects onα-microtubles and microfilaments of goat oocytes.

抑制24h转为正常培养24h，不影响卵母细胞的成熟和孤雌激活能力，并且CHX抑制后再成熟的卵母细胞经孤雌激活发育到囊胚的比例与对照组卵母细胞相似。8、体外受精后，CHX抑制后再成熟卵母细胞的多精受精现象显著增加，发育到桑椹胚/囊胚的比例显著低于对照组。9、CHX抑制过程中皮质颗粒仍能发生迁移，但是CHX抑制会对皮质颗粒的迁移造成不可恢复的损伤，使再成熟的卵母细胞内皮质颗粒不能完全迁移。10、CHX抑制对山羊卵母细胞α-微管和微丝都没有影响，无论是抑制后处于生发泡期的卵母细胞，还是抑制后再成熟的卵母细胞，微管和微丝的分布都与对照卵母细胞相似。

Can reverse JA-induced migration inhibition JA-treated cells also showed significantly higher adhesion to laminin In conclusion these results indicate a novel anti-tumor role of JA via reducing reorganization of cytoskeleton increasing integrin 3 expression and adhesion ability to inhibit tumor cells migration Furthermore immunofluorescence staining of phalloidin/actin indicated that JA-treated cells exhibited cytoskeleton rearrangement and significant decreases in the number and length of filopodia Activity of Rho-family small GTPases was detected by a GST-pull down assay and showed that GTP-loaded Cdc42 and Rac were decreased in JA-treated cells In the future we hope these findings will be helpful to further understand the mechanisms underlying tumor progression in oral cancer and subsequently improve the cancer prevention and treatment

以抗体阻断integrin ？3可以有效使OC2恢复被JA所抑制的迁移能力。细胞贴附实验指出JA能提高OC-2在laminin环境下的贴附能力。当我们进一步以免疫萤光染色观察细胞，发现在JA处理下的OC-2，其外形、细胞骨架和细胞边缘的filopodia的密度和长度明显减少，利用GST-pull down assay侦测到Cdc42-GTP和Rac-GTP的表现量降低。推论爵床素A可能透过刺激口腔癌细胞大量表现integrin ？3，使细胞贴附能力增加或是透过改变细胞骨架来降低细胞迁移能力。希望本研究对於JA所做的抗癌及抗转移效应评估能提供口腔癌治疗在研发上一些帮助。

An improved ant colony algorithm on the basis of ant colony system to solve the traveling agent problem is presented.Ants secrete multi-pheromones to reflect the change of host's service ability and network load dynamically with the results that agent's migration is more agile.

在蚁群算法的基础上，提出分泌多种信息素的改进蚁群算法来求解旅行Agent问题，动态反应了节点服务能力和网络负载的变化，使迁移更具有灵活性。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。