使结合

The method involves exposing the cells to a compound having the formula I in which: w is a nucleic acid x is a non-amino acid or non-peptide nucleic acid binding group y is a spacer having a chain length equivalent to 1-30 carbon-carbon single covalent bonds or is absent R4 is H or halogen or CH2O-R3; and R1, R2 and R3 are the same or different and are either hydrogen, methyl, ethyl, alkyl, alkenyl, hydroxylated alkyl, hydroxylated alkenyl groups or ether containing alkyl, alkenyl, hydroxylated alkyl or hydroxylated alkenyl groups optionally being an acyl group having a carbon chain length equivalent to 3-24 carbon atoms saturated or unsaturated, with the proviso that at least one of R1, R2 or R3 includes a group having a carbon chain of 3-24 carbon atoms saturated or unsaturated, or to a compound having the formula II in which: w is a nucleic acid x is a non-amino acid or non-peptide nucleic acid binding group y is a space having a chain length equivalent to 1-30 carbon-carbon single covalent bonds or is absent, R5 is alkyl, alkenyl, hydroxylated alkyl, hydroxylated alkenyl group or ether containing alkyl, alkenyl, hydroxylated alkyl or hydroxylated alkenyl group optionally being an acyl group having a carbon chain length equivalent to 3-24 carbon atoms saturated or unsaturated, with the proviso that R5 includes a group having a carbon chain of 3-24 carbon atoms saturated or unsaturated.

该方法包括使细胞暴露于具有式的化合物，在式中：w是核酸，x是非氨基酸或者非肽核酸结合基团，y是具有等于1―30个碳―碳单共价键的链长的间隔基或者不存在，R4是H或者卤素或者CH2O－R3；R1、R2和R3是相同的或者不同的并且是氢、甲基、乙基、烷基、链烯基、羟基化烷基、羟基化链烯基或者包含烷基、链烯基、羟基化烷基或羟基化链烯基的醚，任选地是来源于具有等于3―24个碳原子的碳链长的饱和或不饱和脂肪酸的酰基，其条件是R1、R2或者R3的至少一个包括具有3―24个碳原子的饱和或不饱和碳链的基团，或者使细胞暴露于具有式的化合物，在式中：w是核酸，x是非氨基酸或者非肽核酸结合基团，y是具有等于1―30个碳―碳单共价键的链长的间隔基或者不存在，R5是烷基、链烯基、羟基化烷基、羟基化链烯基或者包含烷基、链烯基、羟基化烷基或羟基化链烯基的醚，任选地是具有等于3―24个碳原子的饱和或不饱和碳链的链长的酰基，其条件是R5包括具有3―24个碳原子的饱和或不饱和碳链的基团。

The writer combines his/her own teaching practice and tries from the angle of agrestic geography teaching and put forward some throw the opinion that the brick leads jade namely a, combine physically, pay attention to the mutual contact of knowledge and permeate mutually to make the student understand country feeling and state of the nation; Two, analyze the typical model agrestic case to stir up the student's patriotic enthusiasm; Three, open the teaching activity outside the lesson that the exhibition enriches to make the student is in controling knowledge foundation and slice body to check and feel the development and variety of home town; Four, the teacher wants peacetime to much "burn joss stick" and pays attention to the backlog of knowledge, for the convenience of teaching in at random the Nian come and be used.

摘 要：开展国情教育，是中学教育界面临的一个新课题。笔者结合自己的教学实践，试图从乡土地理教学的角度，提出几点抛砖引玉的意见即一，结合实际，注重知识的互相联系与相互渗透，以使学生了解乡情和国情；二，剖析典型乡土事例，以激发学生的爱国热情；三，开展丰富的课外教学活动，以使学生在掌握知识基础上，切身体验和感受家乡的发展与变化；四，教师要平时多"烧香"，注重知识的积累，以利教学中信手拈来，派上用场。

HA increased level of NO by activating NOS after combining with H〓 and H〓 receptors; and on the one hand 5-HT caused brain microvessel endothelial cells constracting strongly, made the tight joint opening, increased the permeability of BBB, and on the other hand 5-HT increased the quantity of pinocytosis vesicle in endothelial cells and enhance the permeability of BBB after comining with 5-HT〓 receptor.

HA与H〓和H〓受体结合，激活NO合成酶，使NO含量增多而诱发BBB通透性增加；5-HT与5-HT〓受体结合，一方面引起脑微血管内皮细胞强烈收缩，使内皮细胞间的紧密连接开放，BBB通透性增高，另一方面可引起内皮细胞转运小泡增加，BBB通透性增高。

This is caused by the intercalation of MB to DNA bases which restricts the rotational motion of MB.

溴化乙锭的存在使MB-DNA体系的荧光强度增强，这是由于溴化乙锭与MB竞争结合DNA而使MB与DNA的结合反应向左移动，溶液中游离MB浓度增加了的缘故。

It will expose the design problem of maintainability of civil airplane to the early stage of design. This paper discusses the composing of the virtual maintenance environment, builds non-immersion virtual maintenance environment of undercarriage, and establishes a part library management system. The characteristic of maintainability models and maintainability analysis based on virtual technique is researched to give the maintainability frame model of civil airplane virtual maintainability analysis. All levels of maintenance time model and maintenance man-hour model are established. Analog-Huffman code is proposed to optimize space partition method in traditional collision detection arithmetic."Maintenance therblig"is brought into the broken down of maintenance tasks, and the new maintenance tasks broken down method is proposed. The demands of human measure, position, and equipment layout in human engineering are discussed, and the verification methods of human measure, position and equipment layout in virtual maintenance are given. Basic principle and verification procedure of maintainability virtual verification is developed primarily, and the reachablity, the visibility, and posture-comfortability are verified.

本文论述了虚拟维修环境的组成，构建了某型飞机主起落架非沉浸式虚拟维修环境，建立了维修对象的零件库管理系统；研究了基于虚拟技术的维修性模型特点及维修性分析流程，给出了基于虚拟技术的民机维修性分析框架模型，并结合传统维修性模型，建立了各级维修时间模型和维修工时模型；提出类HUFFMAN编码，结合人因工程的要求，优化了传统碰撞检测算法中的空间分割法，使之适用于柔体；在维修性分解中引入"维修动素"，给出了与CATIA、DELMIA等应用软件结合紧密的维修任务分解方法；论述了人因工程中对人体尺寸、姿势、设备布局的要求，给出了虚拟维修中人体尺寸、姿势分析、民机内设备布局的验证方法；初步制订了维修性虚拟验证的基本原则和验证程序，并结合具体实例对可达性、可视性、干涉性以及人体姿态舒适性进行了验证，较清晰、真实地反映了维修人员的维修状态。

The RhoA oncogene , have been revealed in recent years, are closely related to various malignant tumours. RhoA, belonging to Rho family, is one member of the ras superfamily, whose encoding production, namely RhoA protein, pertaining to micromolecular mass GTP conjugated protein family, in GTP conjunctive active form and GDP conjunctive unreactive form. It exist in the latter form in normal cells and the switch of the two forms enables them to educe functions, paralleling"molecular switch", the diminutive G protein in activated state being able to activate the wink dromo-iter to work functions, which is in the resemblance of process during which the Ras genic mutation induces its continous activations.

RhoA是近年来新发现的与多种恶性肿瘤密切相关的癌基因，RhoA属于Rho亚家族，是ras基因超家族的一员，其编码产物RhoA蛋白属于小分子量GTP结合蛋白族，分为GTP结合的活性型和GDP结合的非活性型两类，在正常细胞内以GDP结合的非活性型存在，两种活性形式之间的转换使他们能够发挥一种类似"分子开关"的作用，活性态的小G蛋白能激活下游的信号传导通路，行使其功能。

L Drapable dimension in cotton/silk blends棉与真丝混纺悬垂织物l Subtle tonalities in stripes条纹织物的优雅色调l Knit ottoman with a compact appearance紧凑外观的棱纹织物 Home家纺系列 An Unrehearsed balance combines greys with a whitened aqua and strident fuchsia.

灵感乍现：灰色基调结合浅绿色和紫红色天赋横溢：蓝色基调结合深红色和香草蓝暴露之美：阴柔的海洋色结合热情的黄绿色规则之外：棕色，黄褐色和金色；深紫色则使整个色系更为丰满理解境界：橙红色、凫蓝色结合桃红和陶

NodD binds to and bends target promoters through anchoring two tandem and individual specific DNA sites. NodD functions as a tetramer, which has a V-shaped main body. Tetrameric NodD is to change its own conformation rather than its oligomeric forms in response to small signal molecules. The specific interaction between each NodD DNA-binding domain and each specific DNA site does not alter itself in spite of naringenin induction, and the induced conformational change is transferred from protein to DNA. Only the DNA conformation incited by induced NodD is competent for RNA polymerase to form the transcriptional open complex. It cannot be excluded that NodD may have protein-to-protein contacts with RNA polymerase, and that the NodD conformational change may also directly contribute to the transcriptional open complex formation. However, the NodD conformational change itself cannot serve as the determinant of the transcriptional molecular switch.

通过研究，我们提出了初步的NodD操纵子激活模型：第一，四聚体是NodD蛋白的功能单位，它通过铆钉两个串联的相对独立的DNA靶位点结合被诱导的启动子；第二，小分子配基的结合是改变NodD四聚体的构象而不是引发不同形式的寡聚体，在我们的模型中，NodD四聚体缩小其V形主体的弯折角，进而缩短其DNA结合功能域的间距；第三，小分子信号的诱导并没有改变NodD的DNA结合域和其DNA靶位点的相互作用，NodD的构象改变由蛋白质经其双铆钉位点传递给DNA；第四，只有诱导状态的NodD激发的DNA构象才能有效地使RNA聚合酶形成转录开放复合物；第五，不排除NodD与RNA聚合酶可能有直接的相互接触位点，不排除NodD构象的改变可能直接有利于RNA聚合酶形成转录开放复合物，但是我们认为NodD构象改变本身不是充当转录激活开关的决定因素。

However, fully consideration of special structure and physiological function of CRP is critical to investigate whether CRP provide protect effect or pathogenic effect in the progression of atherosclerosis. CRP is a member of pentrxins family, which consists of five identical, noncovalently associated 23-kDa protomers arranged symmetrically around a central pore with a 102 A external diameter. Each protomer has a recognition face with a phosphocholine binding site consisting of two coordinated calcium ions adjacent to a hydrophobic pocket. The opposite face of the pentamer is the effector face, where complement Clq binds and Fc receptors are presumed to bind. A cleft extends from the center of the protomer to the central pore of the pentamer. Both faces ofpentamer form a special bi-phase structure: one face is the ligand recognition phase, which can recognize apoptosis cells and enzyme modified low density lipoprotein in which PCh is exposed.

CRP是一种五聚体蛋白，外径为102 A，由5个相同的单体以非共价键结合，形成双面的环形结构，每个单体的配体识别位点或受体结合位点分别位于五聚体两个平面上，分别组成配体识别相和效应器相，从而构成特殊的二相性结构：一面为配体识别相，含有磷酸胆碱(phosphorycholine，PCh)结合位点，能够使CRP识别在病理条件下暴露出PCh的凋亡细胞、酶修饰低密度脂蛋白(enzyme modified low density lipoprotein，E-LDL)等。E-LDL是天然低密度脂蛋白(native Low density lipoprotein，N-LDL)在多种蛋白酶，如胰蛋白酶、神经氨酸酶、胆固醇酯酶等作用下的代谢产物，其主要特点是结构发生改变(N-LDL大小均匀，平均直径250±30 A，E-LDL大小差异极大，直径为100-2000 A)，暴露磷酸胆碱位点，能够被CRP识别；另一面为效应器相，能够与巨噬细胞、单核细胞表面受体FcγRⅡa结合，介导巨噬细胞、单核细胞识别及吞噬凋亡、坏死细胞作用。

The RhoA oncogene , have been revealed in recent years, are closely related to various malignant tumours. RhoA, belonging to Rho family, is one member of the ras superfamily, whose encoding production, namely RhoA protein, pertaining to micromolecular mass GTP conjugated protein family, in GTP conjunctive active form and GDP conjunctive unreactive form. It exist in the latter form in normal cells and the switch of the two forms enables them to educe functions, paralleling"molecular switch", the diminutive G protein in activated state being able to activate the wink dromo-iter to work functions, which is in the resemblance of process during which the Ras genic mutation induces its continous activations.

RhoA是近年来新发现的与多种恶性肿瘤密切相关的癌基因，RhoA属于Rho亚家族，是ras基因超家族的一员，其编码产物RhoA蛋白属于小分子量GTP结合蛋白族，分为GTP结合的活性型和GDP结合的非活性型两类，在正常细胞内以GDP结合的非活性型存在，两种活性形式之间的转换使他们能够发挥一种类似&分子开关&的作用，活性态的小G蛋白能激活下游的信号传导通路，行使其功能。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。