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使纯化

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The results showed that the purified recombinant protein under denaturing conditions was refolded in Ni-NTA column by using refolding buffer.

实验结果表明,在变性条件下纯化的样品在柱上不经洗脱,直接用复性液洗涤6h,可以使EKLC在得到提纯的同时实现复性,得到了电泳纯度为96%的具有生物活性的EKLC,最高活性为712U。

While purists may consider the second way as a cheat, not really a cross country road trip, each individual needs to ask themselves whether they want to prove they have the endurance make the entire trip in one shot, or to see the country from the seat of a bike.

虽然纯化,可考虑采用第二种方式的欺骗,而不是真正的越野之旅,每个人必须问问自己,是否他们想证明他们的耐力使整个行程的一杆,或者看到了该国的所在地的自行车。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。