使纯化

Take the 10~12ml single-valve capacity as the example, with flings turns the head to separate evenly, 36.000rpm 60 the hour, turns the head with the angle type to separate 45, OOOrpm 36 the hour, the former including adds the deceleration to use in common 130,000,000 revolution of actuation department lives, the latter must use 100,000,000 revolution of actuation department lives, this was 100 ~ 20,000,000,000 transfers to at that time the supercentrifuge total life looked, without doubt each time tests the expense to be excessively high, in addition the CsCl amount used are many, price expensive and so on factors, causes this kind of separation purification work to become the very expensive experiment.

以10~12ml单管容量为例，用甩平转头分离，36.000rpm 60小时，用角式转头分离45，OOOrpm 36小时，前者包括加减速在内共用去1.3亿转驱动部寿命，后者也要用去1亿转驱动部寿命，这对当时超速离心机总寿命为100~200亿转来看，无疑每次实验费用过高，加上CsCl用量多、价格贵等因素，使这类分离纯化工作成为非常昂贵的实验。

Essential oils from the dried rhizomes of zingiber officinale were extracted by supercritical-CO2 fluid(SFE-CO2 ),the flavonoids of essential oils were further extracted by solvent.

利用超临界CO2流体从干姜中萃取出姜精油，经过有机溶剂对其萃取使生姜黄酮进一步分离，采用大孔树脂为层析柱填充料对生姜黄酮进行纯化，得到纯度为72.3%生姜黄酮。

Then gel filtration, positive ion resin absorption and isoelectric deposition were applied for polysialic acid purification process. The purity of polysialic acid was above 90 % with neutralisation deposition method and the recovery yield of polysiac acid was 55.8%.

探讨了凝胶过滤法、阳离子树脂吸附法、中和沉淀法进一步纯化聚唾液酸的工艺，其中中和沉淀法可使聚唾液酸纯度达到91.5%，回收率为55.8%。

The protein purified with Ni-NTA column was denatured by 8 mol/L urea and dialyzed for refolding.

Ni-NTA柱纯化后的特异性蛋白经8 mol/L脲素变性，梯度透析使蛋白质复性。

And just as prayer was ennobled by the influence of the Cabala, so did most ritual actions cast aside their formalism, to become spiritualized and purified.

正如祷告，是高贵，所影响的cabala势，所以多数礼仪行动抛开形式主义，使他们成为spiritualized和纯化。

ResultsWith polyamide resin to absorb and purify, eluted with 40%,60%,70%,80% ethanol, good results were obtained.

结果通过聚酰胺树脂富集与纯化，并采用40%，60%，70%，80%乙醇梯度洗脱，可以使芦荟蒽醌衍生物得到较好的分离。

There is still no effective method on effectively curing this disease. In this paper, egg-yolk antibody against WSSV was prepared and its effectiveness on preventing and curing this disease was tested. In order to get adequate WSSV antigen, the differential-speed centrifugation and the sucrose gradient ultracentrifugation were used to isolate and purify WSSV from the foregut and gill tissues of shrimps affected with WSSV.

为了获得足量的WSSV抗原，本文以感染WSSV的对虾前肠和鳃等组织为材料，分别利用差速离心法和蔗糖密度梯度离心法进行了WSSV的分离纯化，结果表明：利用差速离心法虽然能够使所分离的WSSV的完好率达70﹪左右，但其中杂质较多，病毒粒子的收获量较少；利用蔗糖密度梯度离心法提取WSSV，虽然其完好率稍低于差速离心法，但也可以达到60%以上，并且，所得到的WSSV的量要大大高于差速离心法，适合于WSSV的大量分离。

It took several years to develop an industrial purification process that removed contaminants such as PCBs (polychlorinated bi-phenyls), pesticides, cholesterol, polyunsaturated fatty acids, and vitamins A and D from the shark liver oil, while at the same time concentrating the alkylglycerols without destroying them.

花了好几年，人们才推出了纯化工艺，以除去鲨鱼肝油中的PCBs，农药，胆固醇，多不饱和脂肪酸，维他命A和维他命D等杂质，同时使alkylglycerols得到浓缩却不受破坏。

The purified human recombined TNFαwas solidated, and antibodies against TNFαdisplayed on phage was enriched on it by five rounds of biopanning, including attach-elute-proliferation, from the pooled human antibody combinatorial libraries .

将纯化的人重组TNFα固相化，用混合的噬菌体人源抗体库(HIV、HAV 和HBV)进行五轮&吸附-洗脱-扩增&筛选，使能够与TNFα特异性结合的噬菌体抗体得到富集。

It is implemented by adding power adjustment ark on electrical heater which makes power is adjustable,and connecting electrical heater and steam heater in series which can save power and meet production requirements.

介绍对铅冶炼生产中制氧装置纯化系统进行的技术改造，电加热器增设调功柜，使其功率可调，采用电加热器与蒸汽加热器串联的方式，既节省电能，又可满足生产要求。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失