使糖化

In comparison to the vector containing 4-exa gene without spacer peptide, the expression level of vectors containing glycosylation site sequence and enterokinase recognition site sequence as spacer was increased by 4 and 9 times, respectively.

在EXA的N端增加间隔肽可促进蛋白分泌，与不含间隔肽序列的4拷贝EXA串联表达载体相比，含有糖基化位点和肠激酶识别位点间隔肽的插入，使蛋白表达水平分别提高了4倍和9倍。

Studies also indicated that genistein exsists not only in soybeans but also in many kinds of plants such as vegetables and fruits.As a potential anticancer agent, the actional mechanisms of genistein mainly includes as follows:First, genistein can depress the activity of protein tyrosine kinase and transduction pathways for the phosphorylation of receptors and mitosis signal. So genistein can lead to cells' proliferation depressed. Second, genistein has minimal effects of phytoestrogens. It can be combined with estrogen receptor and improve the synthesis of cellular sex hormone binding glulobin, and improve the activity of UDP-glucuronyl transferase. Through these pathways, it can inhibit the cell activity of breast cancer and prostate cancer.

作为一个很有潜力的抗肿瘤物质，三羟异黄酮的作用机制主要包括：①抑制蛋白酪氨酸激酶活性，可阻抑PTK引起的受体磷酸化和有丝分裂的信号传递，导致癌细胞增殖受抑；②弱雌激素作用：可通过与体内雌激素受体结合，并可增加细胞内性激素结合球蛋白的合成，增加UDP-葡糖醛酸转移酶的活性等途径抑制乳腺癌和前列腺癌细胞活性；③拓扑酶Ⅰ和Ⅱ抑制剂，抑制细胞活性；④上调细胞周期性负性调节因子P21WAF1/CIP1的表达，使之负性调节因子作用增强；⑤可阻止胰岛素样生长因子-Ⅰ、肝细胞生长因子和神经生长因子的作用而抑制肿瘤生长；⑥其他：抗氧化作用、抑制热休克蛋白、诱导细胞凋亡、抑制新生血管生成和抑制多种耐药相关蛋白等。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

The experimental results revealed that papain, calcium chloride, compound phosphate, and glucide keeping humidity reagents intensify the tenderness of pork jerky, but the addition should be proper. The best proportion was papain0.4‰, compound phosphate 0.4%, calcium chloride 0.1%, and sorbitol 1.5%.

实验结果表明：木瓜蛋白酶、复合多聚磷酸盐、氯化钙和糖类保湿剂都可增加肉脯的嫩度，但用量应适当，添加量过大，反而使肉脯的感官品质变差；肉脯嫩化的最佳配比为木瓜蛋白酶0.4‰、复合多聚磷酸盐0.4%、氯化钙0.1%、山梨糖醇1.5%。

Glucocorticoid: For early skin swelling and serositis, myositis, interstitial lung inflammation, and so effective, and for vascular damage and fibrosis invalid, side effects of high-dose applications, especially for the existing kidney damage Patients easily add vascular damage, and even cause acute renal failure, it is necessary to be careful when using the dosage and the treatment should be individualized, based on clinical performance.

糖皮质激素：对于早期皮肤的肿胀及浆膜炎、肌炎、肺间质炎症等有效，而对于血管损害和组织纤维化无效，大剂量应用时副作用大，尤其是对于已有肾脏损害的患者易加重血管损害，甚至引起急性肾功能衰竭，故使用时须慎重，使用剂量及疗程应个体化，依据临床表现而定。

In this new strategy, selective glycosylation of indolecarbazole can be achieved using unprotected, unactivated glucose.

采用了新的糖苷化手段，可以使吲哚并咔唑直接与未保护和活化的葡萄糖作用选择性的生成β糖苷键，省略了糖的多步保护、脱保护过程，大大简化了操作。

Moreover, MeJA induced defense genes, such as pathogenesis-related proteins (PR2a), phenylalanine ammonia-lyase, BX9, and TPS. MeJA also increased DIMBOA and Vannillic acid content of leaves and roots significantly but leaf p－Hydroxybenzoic acid concentration was decreased significantly and Protocatechuric acid was produced in roots only.

1MeJA叶片处理能诱导叶片和根系中丙二烯氧化物环化酶基因、糖基转移酶（BX9）基因、苯丙氨酸转氨酶基因的表达，使叶片和根系中丁布含量升高，同时，6μL/L MeJA处理使第二叶中原儿茶酸含量显著降低，诱导根中产生原儿茶酸，使第二叶中香草酸含量和根中未知物质峰面积显著增大。

Feeding for 24h by third instar larvae of the maize stalk borer induced farnesyl pyrophosphate synthase gene expressing, but had no effect on expression of AOC, BX9, PAL and PR2a genes and DIMBOA content.

3玉米螟3龄幼虫处理24h能诱导玉米根中法呢烯基焦磷酸合成酶基因表达，但不能诱导丙二烯氧化物环化酶、β－1，3葡聚糖酶基因（PR2a）、糖基转移酶（BX9）、苯丙氨酸转氨酶基因表达，同时对第二叶和根中丁布含量无显著影响，使第二叶对羟基苯甲酸及第三叶丁布、对羟基苯甲酸、香草酸含量显著降低。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。