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To compensate the descent of pH for the heat-up, exalting the Tris-HCl buffer's pH can avoid the abscission of the purine and make the long sequence PCR go on smoothly. The GC-rich template's secondary structure is complex and stable. The organic reagent methylamine contributes to uncoil the secondary structure.The pfu DNA polymerase has more fidelity and thermostability than Taq DNA polymerase.

高GC含量使模板二级结构复杂而稳定,一般热启动难于解开,而变性温度过高、时间过长又不利于DNA聚合酶的活性作用发挥,于是借助能缓释模板二级结构的有机试剂甲胺扩增高GC含量模板。pfu DNA聚合酶比Taq酶有更高的保真复制性能和热稳定性,更有利于高GC含量模板的扩增。

Based on the application of the sensor monitoring technology, the sensor supplied by the BALLUFF company of Germany has been used to control and adjust the slice of the headbox of the paper machine, The gap between the upperlip and underlip of the slice is able to be adjusted accurately and stably, therefore stable paper quality is guaranteed.

摘 要:应用传感测控技术对堰板进行调节控制,使堰板的上唇板和下唇板间的开口度及调整位置准确和稳定。

Release membrane tension controller used to make high-speed release membrane stability, uniform intensity.

放膜采用张力控制器控制,能使高速放膜稳定,力度均匀。

The simulation results show that this method can make PWM rectifier run with unit power factor, the output DC voltage stabilize on the desired value with the fast dynamic response.

仿真结果表明,该方法能使所设计的PWM整流器运行于单位功率因数,输出的直流电压基本稳定在期望值且具有快速的动态响应,满足了设计要求。

Superior geographical environment, strong technical force, unique production process technology, modern equipment and the advanced and practical stable and reliable product quality, so I became unshaped refractory, light burning stereotypes Products, one of professional manufacturers.

优越的地理环境、雄厚的技术力量、特有的生产工艺技术、现代化的先进实用装备和稳定可靠的产品质量,使我公司成为不定型耐火材料、轻烧定型产品的专业生产厂家之一。

It results in unstability of combustion and even structure destruction because of system resonance.

它引起燃烧的不稳定,使燃烧效率下降,甚至导致系统共振,结构破坏。

It makes it a very unstable thing.

它使一个非常不稳定的事情。

Removal of the tumour might interfere with balance, leading to 'vertigo' an unpleasant feeling of unsteadiness.

该肿瘤的切除会阻碍人的平衡,导致眩晕(使人产生不稳定的一种不愉快的感觉)。

On one hand, each language develops harmoniously in this ecosystem. On the other hand, each language competes with each other. The changes of one or more languages will put the language cultural ecosystem in an unsteady state, and then causes the vicissitudes of language culture.

在这个系统中,各民族语言一方面相互协同发展;另一方面各民族语言也存在相互竞争,任何一个或几个民族语言的变化都会使语言文化生态系统处于不稳定状态,继而引起语言文化的变迁。

China has been the first biggest foreign exchange reserved country in the world. With increase of foreign exchange reserve, for the stable of RMB valuta and relieve the international upvalue stress from foreign exchange market for foreign supply is more than need, central bank have to purchase foreign currency for forming huge money supply amount and increase inflation stress.

随着外汇储备的增长,为了维持人民币币值的稳定,减轻外汇市场上由于外汇供给大于需求而产生的人民币升值压力,央行不得不购进外汇,投放基础货币,从而使外汇占款大幅增加,形成巨大的货币供应量,加大通货膨胀的压力。2004年物价的大幅上涨,和外汇储备的快速增长密不可分。

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And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47:5 法老对约瑟说,你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法,同时,采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。

Hey Big Raven, The individual lies dont matter anymore - its ALL a tissue of lies in support of...

嘿大乌鸦,个别谎言的事不要再-其所有的组织的谎言,在支持。