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使浸润

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By using electroporation transfects,the TGF〓expression vector were introduced into HL-60 cells.Thecells were allowed to grow for 1 day before beingsubjected to selection for the ability to grow in mediumcontaining 600ug/ml geneticin.Stable cell clones resistantto G418 sulfate were obtained after 2-3 WK growth withdrug-containing medium.Southern blot hybridization wascarried out.RNA slot have higher expression of TGF〓mRNA in HL-60 transfected.All results followed suggestedthat TGF〓 have suppressed the maliganant phenotype of HL-60 cells.

利用电击法将〓表达载体导入HL-60细胞中,经G418筛选培养20天后再行Southern杂交检测外源〓整合情况,利用RNA狭线杂交法证明了在转录水平上表达有所提高,进一步进行了流式细胞仪测定DNA含量,使细胞在〓期产生生长阻滞作用,细胞生长速度,在软琼脂中成集落数、在组织中的浸润性及在裸鼠中的成瘤性均发生变化,我们的实验结果表明TGF〓对HL-60细胞具有抑制恶性表型的作用。

The histopathological changes were observed at the end of the experiment,the results showed that the significant increases in the thickness of mucosa、RAS and parietal cell number were present in TL(0.4 or 0.8 g/kg,ig)groups after prolonged effect of 0.015% ammonia water on gastric mucosa in rats,the mucosal epithelial cell and parietal cell lesions were significantly decreased in the TL group when compared with the model group.

结果表明,灌胃TL0.4及0.8 g/kg,可使粘膜及壁细胞的损伤、胃体部及胃窦部的炎细胞浸润症状、粘膜及PAS阳性层厚度、壁细胞计数等各指标较模型组有显著改善。

Results (1) Clinical characteristics included abdominal pain,diarrhea,abdominal distension,nausea,vomiting,low heat and weight,etc.Clinical characteristic symptom relieved with rectification of ketosis and ideally controlling of blood sugar;(2)The counts of acidophil leukocyte in blood and marrow dropped with the relief of symptom ;(3) Endoscopic features concluded mucosal erosion and hydrops,involved the whole stomach,lack of specificity,gastric antrum and ileocecum were more invaded,lots of acidophil leukocytes were seen in biopsy;(4)Hormone,insulin were the first choice to treat eosinophilic gastroenteritis,which could relieve symptom rapidly and reduce acidophil leukocyte to normal;(5)If the diseases recur again and again,the patients should adopt more treatment time,use insulin long time or use immuno-suppressive agent.

结果 (1)患者的临床表现样,包括腹痛、腹泻、腹胀、恶心、呕吐、低热及体重下降等糖尿病酮症的表现;临床症状的缓解随酮症的纠正和血糖控制理想而缓解;(2)外周血和骨髓中嗜酸细胞计随着症状的缓解而下降;(3)内镜下表现多黏膜糜烂和水肿,累及全胃,缺乏特异性,以胃窦和回盲部最常受累,活检可见量嗜酸细胞浸润;(4)激素、胰岛素应用为治疗的线药物,可迅速缓解症状,并使嗜酸细胞恢复正常;(5)病情反复复发的患者可考虑延长激素的治疗时间、长期应用胰岛素或免疫抑制剂。

Results (1) Clinical characteristics included abdominal pain,diarrhea,abdominal distension,nausea,vomiting,low heat and weight,etc.Clinical characteristic symptom relieved with rectification of ketosis and ideally controlling of blood sugar;(2)The counts of acidophil leukocyte in blood and marrow dropped with the relief of symptom ;(3) Endoscopic features concluded mucosal erosion and hydrops,involved the whole stomach,lack of specificity,gastric antrum and ileocecum were more invaded,lots of acidophil leukocytes were seen in biopsy;(4)Hormone,insulin were the first choice to treat eosinophilic gastroenteritis,which could relieve symptom rapidly and reduce acidophil leukocyte to normal;(5)If the diseases recur again and again,the patients should adopt more treatment time,use insulin long time or use immuno-suppressive agent.

结果 (1)患者的临床表现多样,包括腹痛、腹泻、腹胀、恶心、呕吐、低热及体重下降等糖尿病酮症的表现;临床症状的缓解随酮症的纠正和血糖控制理想而缓解;(2)外周血和骨髓中嗜酸细胞计数随着症状的缓解而下降;(3)内镜下表现多为黏膜糜烂和水肿,累及全胃,缺乏特异性,以胃窦和回盲部最常受累,活检可见大量嗜酸细胞浸润;(4)激素、胰岛素应用为治疗的一线药物,可迅速缓解症状,并使嗜酸细胞恢复正常;(5)病情反复复发的患者可考虑延长激素的治疗时间、长期应用胰岛素或免疫抑制剂。

Clinical characteristic symptom relieved with rectification of ketosis and ideally controlling of blood sugar;(2)The counts of acidophil leukocyte in blood and marrow dropped with the relief of symptom ;(3) Endoscopic features concluded mucosal erosion and hydrops,involved the whole stomach,lack of specificity,gastric antrum and ileocecum were more invaded,lots of acidophil leukocytes were seen in biopsy;(4)Hormone,insulin were the first choice to treat eosinophilic gastroenteritis,which could relieve symptom rapidly and reduce acidophil leukocyte to normal;(5)If the diseases recur again and again,the patients should adopt more treatment time,use insulin long time or use immuno-suppressive agent.

结果 (1)患者的临床表现多样,包括腹痛、腹泻、腹胀、恶心、呕吐、低热及体重下降等糖尿病酮症的表现;临床症状的缓解随酮症的纠正和血糖控制理想而缓解;(2)外周血和骨髓中嗜酸细胞计数随着症状的缓解而下降;(3)内镜下表现多为黏膜糜烂和水肿,累及全胃,缺乏特异性,以胃窦和回盲部最常受累,活检可见大量嗜酸细胞浸润;(4)激素、胰岛素应用为治疗的一线药物,可迅速缓解症状,并使嗜酸细胞恢复正常;(5)病情反复复发的患者可考虑延长激素的治疗时间、长期应用胰岛素或免疫抑制剂。

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