使杂交

To produce by the mating of individuals of different breeds, varieties, or species; hybridize .

使杂交通过不同品种、变种或种类的个体交配而产生；使杂交

The key points of DGA is to start evolution with initial population evenly distributed in searching space to avoid the leakiness of subspace, to accelerate the searching process by directed crossover and mutation, to improve holistic survival and adaptive ability by means of competition and parallel evolution in genetic system. However, the crossover and mutation act on different lays in space made the searching uniform.

其基本思路是以空间上均匀分布的初始点展开演化以避免子空间的遗漏，以有方向性的杂交和变异加速和调节搜索，同时杂交和变异作用在不同层面上使搜索在整体上均匀化，通过遗传系统里的并行竞争提高整个群体的生存适应力，从而达到整体优化的目的。

The breeding scale of hybrid snakehead (Channa argus♂×Channa maculate♀)waslarger and larger in south of China. Three important factors witch lead to low survivalrate was studied, including prey density, stocking density and cannibalism.

杂交鳢是乌鳢父本和斑鳢母本，通过杂交获得的F_1代，在苗种培育时期易发生残食现象，常使存活率大大降低，严重制约了杂交鳢大规模养殖和推广。

RNAs were obtained from noninfected and Pr4- and Pr4△35-infected macrophages at 3, 8, and 24 hpi and hybridized to a radiolabeled swine IFN-α cDNA probe.

RNAs 是获得从非受传染的和 Pr4-而且 Pr4 △ 35-受传染的 macrophages 在 3 点， 8，和 24 hpi 而且对 radiolabeled 猪 IFN 使杂交-α cDNA 探针。

The synthesized cDNA is a continuous band less than 6. 0kb shown on x-film through α〓PdCTP which was inserted in the first strain of cDNA. The titer of the Ha-cDNA library was about 1. 5×10〓cfu/ml which could represent all protein-encoding sequences from Hz-AM1 cells; Namely, the cDNA library has representativity. After the successful construction of the Ha-cDNA library, the open-reading frame of vp39 gene of Heliothis armigera was then fused in frame with the Ga14-DNA-binding domain in the pGBT9 plasmid and the fusion protein Ga14-VP39 was expressed in yeast to be used as bait protein.

在成功构建棉铃虫细胞Hz-AM1cDNA文库的基础上，本文接着将棉铃虫核型多角体病毒衣壳蛋白VP39（HaNPV-VP39）基因克隆到酵母双杂交系统(yeasttwo-hybrid system)中的质粒pGBT9上。pGBT9上含有酵母菌转录激活因子GAL4 DNA结合域的编码区（GAL4-DBD），使克隆到质粒pGBT9上的HaNPV-vp39基因与GAL4-DBD基因融合，在酵母菌中表达DBD-vp39融合蛋白，该融合蛋白即为酵母双杂交系统中的诱饵蛋白。

Relationship between Frequency change and target DNA concentration showed a simple saturation curve.

采用膜表面自组装技术，能使DNA探针与膜牢固结合，且分布均匀；探针与靶序列浓度增加均使压电传感器上杂交量增加，但探针浓度过大时反而抑制杂交，靶序列浓度与频率变化之间呈饱和曲线关系。

Chicken and quail intergeneric hybridization is a classic distant hybridization in fowl. In fowl science, chicken and quail belong to Aves, Galliformes, Phasiamidae, Gallus and Coturnix, and this hybridization combination has become the example of researching the incompatibility of different germ cell and sterile of distant hybridization.

鸡与鹌鹑杂交是属间杂交，其杂交种胚胎早期出现大量的死亡，并且杂交种出壳以前雌性胚胎全部死亡，这使鸡与鹌鹑杂交成为研究异种生殖细胞不亲和性和杂交种不育的典型。

In an attempt to promote the efficiency in citrus breeding and estimate the pos- sibility of embryo culture applied to citrus production,we carried out the culture technique in the hybrid embryos(Citrus sinensis Osbeek×Poncirus trifoliata Raf.).

为了提高柑桔杂交育种效率和探讨胚培养在柑桔生产上应用的可能性，我们研究了锦橙×枳(Poncirus trifoliata Raf。)的杂交种子胚培养技术。证明：怀特培养基+IAA(1ppm)+CH(600ppm)和 MT 培养基+GA(1ppm)能使第五个小胚萌发成苗；在 MT 培养基+2，4-D(0.7ppm)+BA(0.23ppm)上，有些胚先形成愈伤组织，然后从原有的胚中生根出苗。

V.使交叉,横过,勾划,错过,反对,杂交 be cross get cross be cross with get cross with bear one's cross come home by the weeping cross return by the weeping cross double cross drive the cross make one's cross on a contract No cross , no crown.

使人毛骨悚然先学爬后学走；按部就班；循序渐进。偷偷离开悄悄混进悄悄地过去偷偷溜出爬上；偷偷逼近爬上；偷偷逼近 215-cross n。

By using electroporation transfects,the TGF〓expression vector were introduced into HL-60 cells.Thecells were allowed to grow for 1 day before beingsubjected to selection for the ability to grow in mediumcontaining 600ug/ml geneticin.Stable cell clones resistantto G418 sulfate were obtained after 2-3 WK growth withdrug-containing medium.Southern blot hybridization wascarried out.RNA slot have higher expression of TGF〓mRNA in HL-60 transfected.All results followed suggestedthat TGF〓 have suppressed the maliganant phenotype of HL-60 cells.

利用电击法将〓表达载体导入HL-60细胞中，经G418筛选培养20天后再行Southern杂交检测外源〓整合情况，利用RNA狭线杂交法证明了在转录水平上表达有所提高，进一步进行了流式细胞仪测定DNA含量，使细胞在〓期产生生长阻滞作用，细胞生长速度，在软琼脂中成集落数、在组织中的浸润性及在裸鼠中的成瘤性均发生变化，我们的实验结果表明TGF〓对HL-60细胞具有抑制恶性表型的作用。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。