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Their relative molecular weights were 4785 and 4386 respectively. And their absorbance peaks were 210.5 nm and 225.4 nm respectively. The bovine and caprine placental peptides could improve immunosuppressive T lymphocyte transformation rate. The immunosuppressive lymphocyte transformation rates in groups with the bovine placental peptide of 1:1×10^3 and 1:1×10^4 (P.05)and with the caprine placental peptides of 1:1×10^2, 1:1×10^3 and 1:1×10^4 (P.01 )increased more remarkably than that in immunosuppression group.

结果 从牛和羊胎盘中成功提取出了胎盘肽,其相对分子质量分别为4785和4386;其最大吸收峰分别为210.5和225.4nm;牛和羊胎盘肽均可使顺铂抑制的兔外周血T淋巴细胞的转化率显著提高,其中牛胎盘肽以1:l×10^3、1:l×10^4 2组吸收度明显高于抑制组(P.05);羊胎盘肽以1:1×10^2、1:1×10^3和1:1×10^3 3组效果最为明显(P.01)。

MethodsThe distilled bovine and caprine placental peptide s were prepared by ultrafiltering and their relative molecular weights were mensurated by improved Tricine-SDS-PAGE eletrophoresis with urea.

结果从牛和羊胎盘中成功提取出了胎盘肽,其相对分子质量分别为4 785和4 386;其最大吸收峰分别为210.5和225.4 nm;牛和羊胎盘肽均可使顺铂抑制的兔外周血T淋巴细胞的转化率显著提高,其中牛胎盘肽以1∶1×103、1∶1×1042组吸收度明显高于抑制组(P.05);羊胎盘肽以1∶1×102、1∶1×103和1∶1×1043组效果最为明显(P.01)。

The growth of Ficus carica test-tube seedlings on MS media was sharply inhibited by NaCl with lethal in 300 mmolL^(-1) NaCl and, in leaf, dramatical increase in sodium content and K(superscript +)/Na(superscript +) ratio, in addition to proline and soluble sugar accumulation in 0~200 mmolL^(-1) NaCl.

将多次继代培养的无花果试管苗接种在含NaCl不同浓度的MS培养基上,300mmolL^(-1)NaCl使试管苗致死。在0~200mmolL^(-1)NaCl的培养基上,试管苗生长显著受到抑制,叶片中Na含量增加,K/Na上升。

Cosmos bipinnatus and Trachymene coerulea exhibited stronger endurance to Pb, however, Pb contamination significantly inhibited Dianthus caryophyllus development, which exhibited the weakest enduring ability.

在Pb浓度为1000 mg kg^(-1)时,铅污染可以刺激波斯菊和翠珠花生长,使其根部和地上部的生物量增加,这两种花卉对铅污染的抗性很强,但铅污染会显著抑制香石竹的生长,香石竹对铅污染的抗性很差。

Accordingly,the casalty accidents of industrial production base of coal,steel and iron of Tonghua City have greatly decreased and the serious accidents have fundamentally been stopped.

通过 6年来的辛勤努力,使通化这个煤炭、钢铁工业生产基地的伤亡事故得到了大幅度下降,基本遏制住了重特大事故,安全生产取得了显著成效

The perimeter of a pile with abnormal section is greater than the perimeter of a pile with circinal section or quadrate section,which makes the friction greater.

在截面面积相等的情况下,异形截面桩的侧面周长比方形、圆形及环形截面桩的侧面周长明显要大,从而使桩侧摩阻力显著增大。

The metal tailing applied test in desiccated circumgyrate kiln of 2500t/d as mineralizer and substitute of clay, indicates metal tailing can increase cement output by 10.07%, save coal by 8.29%, decrease dissipative electricity by 4.88%, the economic, social and environmental benefit is very obvious.

尾矿代粘土和矿化剂在2500t/d新型干法回转窑生产线上的应用结果显示,尾矿配料能使水泥产量增加10.07%,节约煤耗8.29%,降低电耗4.88%,经济效益、社会效益和环境效益显著。

In the pH range of 6~12, the results of the flotation tests indicate that the performance of the new combined cationic collector (CS3:CS1=3) and (CS3:CS2=2) is similar, and they are effective collectors for reverse flotation of the iron ores. The combined cationic collectors exhibited the same collectability as dodecylamine, but better selectivity than dodecylamine. By using the combined collector, the dosage of the collector decreased from 1 10-4mol/L to 1 x 10-5mol/L .

单矿物可浮性试验结果表明,在pH=6~12的范围内,新型组合药剂CS_3:CS_1=3和CS_3:CS_2=2具有相近的药剂性能,在其捕收能力与十二胺相当的前提下,不但选择性较十二胺好,而且还能显著提高药剂的协同效应,使药量从单用时的1×10~(-4)4mol/L降至1×10~(-5)mol/L,是铁矿石反浮选的高效捕收剂。

They are: conceptual meaning (logical, cognitive, or denotative content), connotative meaning (what is communicated by virtue of what language refers to), social meaning (what is communicated of the social circumstances of language use), affective meaning (what is communicated of the feeling and attitudes of the speaker/writer), reflected meaning (what is communicated through association with another sense of the same expression), collocative meaning (what is communicated through association with words which tend to occur in the environment of another word), thematic meaning(what is communicated by the way in which the message is organized in terms of order and emphasis).

在商务英语创意中常用的联想方式有接近联想、相似联想、对比联想和关系联想四种。合理地运用联想这一心理现象进行商务英语创意可以引起受众的关怀,影响受众的情绪与行为,使商务英语的效果更直接、更显著。从关联与认知语境的视角出发探讨联想在商务英语创意中的运用是有必要的。关联理论认为,话语的关联程度依赖于语境效果和处理努力,语境效果与关联成正比,处理能力与关联成反比。作者把处理努力理解为认知语言环境所消耗的脑力,关联性越强,话语就越直接,认知所耗的脑力越小,给受话者带来的认知负荷就越小;关联性越弱,话语就越隐含,消耗的脑力越大,受话人的认知负荷越大。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实:两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用,并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著,最佳效应浓度为400nM;2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响:①CREG蛋白对VSMC迁移的影响:刮伤实验发现,加入最佳效应浓度的wtCREG和mCREG蛋白24h后,OB2组迁移能力下降,HITASY组无明显变化;细胞外基质金属蛋白酶-2,9(Matrix metallo-proteinase 2,9,MMP2 ,9)明胶酶电泳检测和Western blot检测结果证实,两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2,9减少,而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases,TIMPs)增加;②CREG蛋白对VSMC分化的影响:加入400nM的wtCREG和mCREG蛋白12h后,OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加,LM-1、FN表达减少;③流式细胞仪分析细胞周期和BrDU染色分析证实,加入400nM的wtCREG和mCREG蛋白后,OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572,HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034;3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用:①免疫共沉淀和免疫荧光双染色分析结果显示,CREG蛋白与M6P/IGF2R存在直接结合;②应用抗体阻断实验:将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中,CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱,而且与加入anti- M6P/IGF2R浓度正相关。

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What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢?

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我,一个心中布满虚荣的女孩子真实的想法。

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