使无菌

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

We also discovered that selenium can decrease hematolysis and effects function of vacuole toxin of HP. morphological conversion from spiralto coccoid forms occurredwhen HP was caltured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E. coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E. coli, but can not influence activity and gene equence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

结果：体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；硒能增强重组VacA对胃癌细胞的抑制作用，使细胞分裂指数和集落形成率降低、S期细胞数量减少，G1期细胞总数增加；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

To test the antibacterial effect of MEBO against anaerobic spore-bearing bacillus, anaerobic non-spore-bearing bacillus (Bacteroides fragilis, propionibacterium acnes) and fungi, etc.

本实验观察了MEBO使厌氧芽胞杆菌——破伤风杆菌、厌氧无芽胞杆菌——脆弱类杆菌和痤疮丙酸杆菌、真菌——白色念珠菌等在形态结构、菌落特点和致病性等方面发生变异。

Both the bacterial culture and aseptic filtrate showed activities to inhibit some pathogenic fungi including "Alternaria alternata", and "Fusarium graminearum" on activity plates assay. Antagonistic crude protein extract was obtained by 70% ammonium sulphate precipitation, and it was thermostable, unsensible to proteinase K and pepsin, and capable to induce the hypha lysis and cytoplasm condensation of "F. graminearum".

经平皿测试表明，多粘芽孢杆菌P-FS08菌株及其无滤液对烟草赤星病菌、小麦赤霉病菌等多种植物病原真菌均有抑制作用。P-FS08培养液通过70%硫酸铵沉淀得到具有拮抗活性的蛋白粗提液，其具有良好的热稳定性；对蛋白酶K和胃蛋白酶均不敏感；够使小麦赤霉病菌菌丝断裂，原生质浓缩。

With the aid of an assistant, the foot is inserted into a pillowcase, and the edges of the case are held together with a towel clip. To make a compact mass of these rather voluminous drapes, a sterile bandage is wrapped snugly from the toes proximally to the proximal edge of the towel and pillowcase. Alternatively, a sterile stockinette rolled from the toes to the groin can be used. The area over the knee is cut away as described in the section on draping the edges of the incision. The stockinette is then covered with a sterile bandage to just below the knee.

在助手帮助下将足插入枕套，枕套边缘由巾钳固定在一起，为使其紧凑，可用消毒绷带从足趾缠绕至治疗巾和枕套的近端边缘，也可改用消毒的弹力织物从足趾套至腹股沟，膝关节表面的织物应于切除，如同切口周围铺单中介绍的方法，这种弹力织物在膝关节以下的部分用无菌绷带缠绕固定，其余铺单方法与足部铺单相同。

With the aid of an assistant, the foot is inserted into a pillowcase, and the edges of the case are held together with a towel clip. To make a compact mass of these rather voluminous drapes, a sterile bandage is wrapped snugly from the toes proximally to the proximal edge of the towel and pillowcase. Alternatively, a sterile stockinette rolled from the toes to the groin can be used. The area over the knee is cut away as described in the section on draping the edges of the incision. The stockinette is then covered with a sterile bandage to just below the knee. The remainder of the drapes are placed as described for the foot.

在助手帮助下将足插入枕套，枕套边缘由巾钳固定在一起，为使其紧凑，可用消毒绷带从足趾缠绕至治疗巾和枕套的近端边缘，也可改用消毒的弹力织物从足趾套至腹股沟，膝关节表面的织物应于切除，如同切口周围铺单中介绍的方法，这种弹力织物在膝关节以下的部分用无菌绷带缠绕固定，其余铺单方法与足部铺单相同。

Some immunologists believe that children now growing up in hyperclean, sterile environments are failing to develop immune systems properly because of inadequate exposure to bacteria.

一些免疫学家认为现在的孩子生长的环境太清洁，因为不足以暴露在细菌环境中，这种无菌环境会使免疫系统不能充分的发展。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失