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Bone marrow-derived mesenchymal stem cells are capable of chondrogenesis, making them a possible source of cells for injectable cartilage tissue engineering. There exist different ideas on the ability of mesenchymal stem cells's chondrogenesis in monolayer culture. Because of this, the effect of adult rabbit's bone marrow-derived mesenchymal stem cells chondrogenesis in monolayer culture was studied. The mesenchymal stem cells was isolated from adult rabbit's bone marrow and monolayer cultured. TGF-β1, Vit-C and Dexamethasone were used. Immunohistochemistry analyses and histological staining of H-E, Methylaniline blue and Alcian blue were performed to identify the expression of collagen type Ⅱ and cartilage associated matrix. The results showed that the induced cells expressed and produced collagen type Ⅱ and cartilage associated matrix. This suggests that the differentiation of adult rabbit's marrow-derived mesenchymal stem cells into chondrocyte in monolayer culture is feasible and may be induced by TGF-β1, Vit-C and Dexamethasone.

骨髓基质干细胞的软骨分化潜能使其可能成为可注射组织工程化软骨研究的种子细胞,为探讨体外培养的骨髓基质干细胞在平面诱导条件下软骨分化的可行性,我们进行下列实验:获取并体外平面培养成体兔骨髓基质干细胞,应用TGF-β〓、Vit-C和地塞米松对其软骨分化诱导,诱导后的骨髓基质干细胞行细胞爬片组织学和Ⅱ型胶原免疫组化,结果证实,诱导后骨髓基质干细胞可分泌Ⅱ型胶原,组织学染色可见类似于软骨细胞,由此证明体外培养的骨髓基质干细胞在平面诱导条件下可以软骨分化,其软骨诱导因子为TGF-β〓、Vit-C和地塞米松。

Similarly, the cantilevered canopies of metal clad steel structure and fritted pattern glass merge the wave forms into a series of repeating flowing elements.

悬臂式天蓬采用金属复合钢结构,结合带烧结图案的玻璃,使整栋建筑的波浪形态融汇成一组流动不息的动感元素。

This smaller image allows these binoculars to have very wide fields without the need for large prisms and eyepieces.

这种小的成像本身就会使镜子有更大的视场,从而不必为此设计更大的棱镜和目镜组。

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Do you know, i need you to come back

你知道吗,我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书,王祥生,李德昌。安徽省首次发现嗜群血蜱。

Chapter Three: Type classification of DE structure in Sino-Tibetan languages.

第三章汉藏语&的&字结构的类型划分。