使外转

Besides the conventional rotator cuff tear, acromion plasty, labrum repair, and surgeries for ennis elbow, osteoarthritis, ankylosis of elbow and teoid osteoma, new techniques have been developed including the arthroscopic repair of large tear of rotator cuff and labrum, tendon transposition, arthroscopic removal of benign tumor around the shoulder joint.

每年除完成常规肩袖修补、肩峰成形、盂唇修补等手术，以及网球肘、骨关节病、肘关节僵直、骨样骨瘤等等的手术治疗外，在关节镜下肩袖、盂唇巨大撕裂修补、肌腱转位、肩关节周围良性肿瘤镜下切除等手术领域内也有了长足发展，使患者在国内可以享受国际先进水平的治疗。

CML is cytogenetically marked by the philadelphia chromosome, which originates from a reciprocal translocation between chromosome 9 and 22 and is molecularly marked a chimeric bcr-abl gene, resulting from juxta-positive of the abl proto-oncogene on chromosome 9 with the bcr gene, which is normally located on chromosome 22. The chimeric bcr-abl gene expression an 8. 5kb hybrid mRNA transcript giving rise to a 210-KD fusion protein (P210〓) with increased tyrosine kinase activity. P210〓 plays a key role in the pathogenesis of CML. The continuous cell line K562 was established from the pleural effusion of a 53-year-old female with CML in terminal blast crisis, and was a human erythroleukemia line, contained Ph chromosome.

绝大多数慢粒患者白血病细胞中具有Ph染色体，是由9号染色体长臂3区4带和22号染色体长臂1区1带相互易位形成，即t（9；22），使位于9q〓的c-abl原癌基因在第二外显子的5'端断裂并易位到22 q〓的M-bcr基因第2或第3外显子的3'端，形成异常的bcr-abl嵌合基因，该基因转导出异常的mRNA，编码并翻译出P210蛋白，该蛋白具有很强的酪氨酸激酶活性，使粒细胞发生恶性增殖。K562细胞属于慢粒急变、红白血病细胞株，具有Ph染色体。

Results are as followed:1 Exposure of HELF cells to BP caused c-Jun activation,and increased the activity of MAPK,PI-3K,p53 and cyclin D1 pathway.2 BP-induced c-Jun activation was inhibited by dominant negative mutants of extracellular signal-regulated protein kinase or c-Jun NH_2-terminal kinase,but not by p38,impling that JNK and ERK pathways medicate c-Jun activation induced by BP.3 Overexpression of dominant-negative mutants PI-3K and Akt potently blocked phosphorylations of c-Jun and ERK,but not JNK in response to BP,suggesting that PI-3K/Akt pathway positively regulates BP-induced c-Jun activation through ERK.4 Inhibition of p53 by its chemical or molecular inhibitor markedly increased the phosphorylation levels of c-Jun,Akt and ERK upon BP stimulation,indicating that p53 negatively medicates BP-induced c-Jun activation through PI-3K/Akt/ERK pathway.5 The cell lines expressed TAM67 exhibits no significant affecting normal cell growth properties.6 TAM67 was able to significantly block G_1-S transition and subsequent cell proliferation,suggesting that c-Jun is essential for cell cycle alternations elicited by BP.7 Overexpression of TAM67 impaired BP-induced cyclin D1 activation,decreasing expression of E2F1 and pRb,indicating that c-Jun participates in the modulation of BP-induced activation of cyclin D1/pRb/E2F1 pathway.8 Stably expression of TAM67 led to the increases in the expression levels of p53 and p21,elevating phosphorylation level of p53,clearly indicating that c-Jun regulates p53/p21 pathway activation induced by BRCollectively,PI3K/Akt/ERK pathway mediated BP-induced c-Jun activation through p53-dependent mechanism.

结果显示：1BP刺激细胞可促进c-Jun活化，并伴随着MAPK、PI-3K、p53和cyclinD1通路各组成成分的活性增强。2利用MAPK通路的显性失活突变体分别阻断细胞外信号调节激酶和c-Jun氨基末端激酶活性，均可明显抑制BP诱导的c-Jun活化，但阻断p38活性对BP引起的c-Jun活化无明显影响，提示JNK和ERK通路参与调控BP诱导的c-Jun活化。3过表达PI-3K和Akt的显性失活突变体也可显著抑制BP诱导的c-Jun活化，并降低磷酸化ERK的表达水平，但对磷酸化JNK的表达水平无明显影响，说明PI-3K/Akt通路通过ERK正性调控了BP诱导的c-Jun活化。4p53的化学/分子抑制剂能使BP作用的细胞内c-Jun活性明显增加，并同时诱导Akt和ERK的磷酸化水平的升高，表明p53可通过PI-3K/Akt/ERK通路对BP诱导的c-Jun活化进行负性调控。5随后观察转染细胞的生长情况，发现TAM67对细胞正常生长和形态无明显影响。6稳定表达TAM67可有效抑制BP诱导的S期细胞数的增加，提示c-Jun在BP致细胞周期改变的过程中发挥了重要作用。7TAM67过表达能够抑制BP诱导的cyclin D1活化，降低磷酸化Rb以及E2F1蛋白表达水平，表明c-Jun参与调控BP诱导的cyclin D1/Rb/E2F1通路的活化。8过表达TAM67可使BP刺激的细胞中p53、p21总蛋白以及p53磷酸化的表达水平明显升高，可见c-Jun也参与调控BP诱导的p53/p21通路活化。

CaN Aβ gene silencing can reduce myocardial hypertrophy in cultured cells, si1280 (21bp) of CaN Aβ gene is the most effective target site for siRNA. The method of intrapericardial injection of plasmid, microbubbles and erzymes can improve transfection efficiency of non-viral plasmid with satisfying targeted transfection. But the scope of transfected myocytes is still limited. CaN Aβ shRNA expressing plasmid transfection in vivo by pericardial injection results in decreased CaN Aβ protein expression of small part of myocytes, and CaN Aβ mRNA only shows decreased trend. The dosage of non-viral vector and the parameters of ultrasound energy should be optimized in further study.

结论RNAi技术抑制CaN Aβ基因表达可有效减轻Ald诱导的心肌细胞肥大程度；CaN Aβ基因中si1280(21bp)片段为实现RNAi的更有效片段；微泡+酶类心包腔内注射超声导入的方法可有效改善非病毒载体在体心肌的转染效率，同时具有一定的靶向性，但总的转染范围仍然有限；采用这一方法进一步进行&一肾一夹&心肌肥大模型大鼠在体心肌细胞的CaNAβ的RNAi干预，发现心肌肥大大鼠心外膜下局部心肌细胞CaN Aβ蛋白水平降低，CaN AβmRNA水平虽有下降趋势，但无统计学差异，提示质粒的用量及超声导入的参数有待进一步研究使其优化。

Part II The experiment study on anti-tumour effect of dendritic cells derived from peripheral blood monocyte of patients with oral cancer after pulsed by tumour antigen in different ways. Collected 50ml peripheral blood from each patients and volunteers to induced DC in vitro. We parted the test group and control group into three subgroups respectively, untreated group A, freezed and thawn group B, RNA transfected group C. To group A, after induced DCs for five days , we added 200U/mlTNF-α in each hole and cultured on. To group B, we added protein antigen (DC/ tumor cells = 1 : 1 ,each hole) for 4h at 5d, and then added 200U/mlTNF-α in each hole and cultured on. To group C, transfected immature DC with tumor cell RNA(DC:RNA=1×105/ml: 5ug)at 5d, operated completely with the specification of invitrogen DMRIE-C reagents. After transfection we replaced transfection agent with complete medium, and then added 200U/mlTNF-α in each hole and cultured on.

第二部分口腔癌患者外周血来源的树突状细胞体外负载抗原后抑瘤作用的实验研究无菌采集20例口腔癌患者和20例健康志愿者外周血50ml，分离单个核细胞体外诱导培养DC，并将实验组和对照组细胞各分为三组，A组为未处理组，B组为冻融组，C组为RNA转染组。A组DC在培养5d后，每孔加入TNF-α200U/ml；B组DC培养第5d后，每孔中加入癌细胞蛋白抗原，使DC/肿瘤细胞=1：1，继续培养4h后，每孔加入TNF-α200U/ml；C组将培养至第5d的未成熟DC用肿瘤细胞RNA进行转染(DC：RNA=1×105/ml： 5ug，按照invitrogen DMRIE-C试剂说明书进行操作)，转染结束后用完全培基取代转染剂，每孔加入TNF-α200U/ml。

In all,10 patients were cured after mean follow-up 15.3±12.8 months.The causes of RFCA failure were demonstrated in surgical operation.In 3 patients without structural heart disease,ablation catheters were not located in distinct target sites during ablation of 2 left posteroseptal pathways and 1 right posterior wall pathway.In 2 cases of Ebstein?s anomaly,ablations were failed by serious tricuspid regurgitation and AP at epicardium.In one case with rhumatic valvular disease,ablation catheter did not achieve mitral annulus because of pathologic changes.Other 4 cases with congenital heart disease were associated with anotomical anomaly and serious hemodynamic disturbance.

s畸形合并房间隔缺损，1例为重度三尖瓣返流使消融电极不能稳定贴靠靶点，另1例为AP靠近心外膜面而致RFCA失败；1例风湿性心脏病联合瓣膜病因瓣膜及瓣下结构病理改变致RFCA失败；1例重度二尖瓣返流RFCA失败与病人不能耐受RFCA与AP定位于24区消融导管难以抵达有关；先天性心脏病大房间隔缺损、重度主动脉关闭不全及矫正性大动脉转位右旋心伴三尖瓣返流各1例，RFCA失败与解剖畸形、心内大分流等血液动力学障碍有关。

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2买方如并非亲自签立卖方转让契的承让人，则须披露足够资料，使卖方能在转让契详列所有以任何方式购买或售卖本物业或其中任何权益的确认人、代名人、转购人或其他居间者全部细节（包括身份证号码及详细地址）、十足价款或其他代价，包括佣金、权益保留金、代理费或据任何一方所知，除根据本协议为转让契应向卖方支付的代价外，任何其他已就居间交易向任何人士缴付或付出的金额。倘买方及任何其他居间者未能提供所需资料令卖方满意，则即使第5条有相反规定，卖方仍可拒绝根据本协议向买方以外的任何承让人完成转让，而无须向买方或任何居间者作出赔偿或承担任何法律责任。

The first direct measurement with micrometer piston skirt and the outside diameter Do micrometer stop screw to the locking plate position, and then dial indicator card installed and well well inserted under the initial installation of the amount of cards, directly into the cylinder corresponding cylinder kit, adjust the volume of the cylinder rod out in table inserted length, so that small hands dial indicator cell has about 1 volume of pre-press and lock locking rod inserted mother, at the same time, large dial indicator dial to adjust zero, the last cylinder to adjust the volume of good form under test card inserted rods and do at Anvil micrometer and measured the rod between the big hands dial indicator at this time than the "zero" high value is the piston and cylinder liner with the space.

3先直接用千分尺测量活塞裙部的外径并将干分尺止动螺钉扳转到锁紧位置，再将卡装好百分表和初步安装好插杆的量缸表直接卡入对应气缸套内，调整量缸表插杆的伸出长度，使百分表的小表针有1格左右的预压量并锁紧插杆锁母，同时将百分表的大表盘调整到零，最后将调整好的量缸表的测杆和插杆卡在干分尺的砧座和测杆之间，此时百分表大表针比&零&高的数值即为活塞和气缸套的配合间隙。

Regarding four phase-stepping method, the difference of new instrument is that the original instrument requires four quarter-wave plates and needs to set up eight different angular magnitudes, whereas the new one requires only one quarter wave plate and changes the angle of analyzers.

在三场架设中，有别於一般需四道光路和输出时的四分之一波板，并且只要调整检偏器之角度便可得到三张的包裹相位图；而四场架设里，也只需多一片输出时的四分之一波板，使其中两道光路的结果为圆偏光，此外，仍然只转动检偏器之角度即可，相较传统的四场，除了元件的简化外，角度设定也较简单容易；六场平偏则改善了一般平偏或圆偏只能单一取得主应力角或因四分之ㄧ波板所造成的影响。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。